Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/41917 https://orcid.org/0000-0001-8927-6190 https://orcid.org/0000-0002-5978-2735 https://orcid.org/0000-0002-7405-3928 https://orcid.org/0000-0002-4935-6070 https://orcid.org/0000-0001-9974-711X https://orcid.org/0000-0001-5271-1048 https://orcid.org/0000-0002-4198-5753 https://orcid.org/0000-0002-6232-2174 |
Resumo: | The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos. |
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Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubesNanotubesPelucid zoneTransfectionDNANanotubosDNAThe pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal de Minas GeraisBrasilICX - DEPARTAMENTO DE FÍSICAUFMG2022-05-24T12:18:01Z2022-05-24T12:18:01Z2016-09-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf10.1038/srep335882045-2322http://hdl.handle.net/1843/41917https://orcid.org/0000-0001-8927-6190https://orcid.org/0000-0002-5978-2735https://orcid.org/0000-0002-7405-3928https://orcid.org/0000-0002-4935-6070https://orcid.org/0000-0001-9974-711Xhttps://orcid.org/0000-0001-5271-1048https://orcid.org/0000-0002-4198-5753https://orcid.org/0000-0002-6232-2174engScientific ReportsMichele Munk PereiraAdo Jorio de VasconcelosHumberto de Mello BrandãoLuiz Orlando LadeiraBruno Campos de CarvalhoLuiz Sérgio de Almeida CamargoNádia Rezende Barbosa RaposoRaquel Varella SerapiãoCarolina Capobiango Romano QuintãoSaulo Rodrigues SilvaJaqueline dos Santos Soaresinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2022-05-24T12:18:02Zoai:repositorio.ufmg.br:1843/41917Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2022-05-24T12:18:02Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes |
title |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes |
spellingShingle |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes Michele Munk Pereira Nanotubes Pelucid zone Transfection DNA Nanotubos DNA |
title_short |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes |
title_full |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes |
title_fullStr |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes |
title_full_unstemmed |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes |
title_sort |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes |
author |
Michele Munk Pereira |
author_facet |
Michele Munk Pereira Ado Jorio de Vasconcelos Humberto de Mello Brandão Luiz Orlando Ladeira Bruno Campos de Carvalho Luiz Sérgio de Almeida Camargo Nádia Rezende Barbosa Raposo Raquel Varella Serapião Carolina Capobiango Romano Quintão Saulo Rodrigues Silva Jaqueline dos Santos Soares |
author_role |
author |
author2 |
Ado Jorio de Vasconcelos Humberto de Mello Brandão Luiz Orlando Ladeira Bruno Campos de Carvalho Luiz Sérgio de Almeida Camargo Nádia Rezende Barbosa Raposo Raquel Varella Serapião Carolina Capobiango Romano Quintão Saulo Rodrigues Silva Jaqueline dos Santos Soares |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Michele Munk Pereira Ado Jorio de Vasconcelos Humberto de Mello Brandão Luiz Orlando Ladeira Bruno Campos de Carvalho Luiz Sérgio de Almeida Camargo Nádia Rezende Barbosa Raposo Raquel Varella Serapião Carolina Capobiango Romano Quintão Saulo Rodrigues Silva Jaqueline dos Santos Soares |
dc.subject.por.fl_str_mv |
Nanotubes Pelucid zone Transfection DNA Nanotubos DNA |
topic |
Nanotubes Pelucid zone Transfection DNA Nanotubos DNA |
description |
The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-19 2022-05-24T12:18:01Z 2022-05-24T12:18:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
10.1038/srep33588 2045-2322 http://hdl.handle.net/1843/41917 https://orcid.org/0000-0001-8927-6190 https://orcid.org/0000-0002-5978-2735 https://orcid.org/0000-0002-7405-3928 https://orcid.org/0000-0002-4935-6070 https://orcid.org/0000-0001-9974-711X https://orcid.org/0000-0001-5271-1048 https://orcid.org/0000-0002-4198-5753 https://orcid.org/0000-0002-6232-2174 |
identifier_str_mv |
10.1038/srep33588 2045-2322 |
url |
http://hdl.handle.net/1843/41917 https://orcid.org/0000-0001-8927-6190 https://orcid.org/0000-0002-5978-2735 https://orcid.org/0000-0002-7405-3928 https://orcid.org/0000-0002-4935-6070 https://orcid.org/0000-0001-9974-711X https://orcid.org/0000-0001-5271-1048 https://orcid.org/0000-0002-4198-5753 https://orcid.org/0000-0002-6232-2174 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais Brasil ICX - DEPARTAMENTO DE FÍSICA UFMG |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais Brasil ICX - DEPARTAMENTO DE FÍSICA UFMG |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Repositório Institucional da UFMG |
collection |
Repositório Institucional da UFMG |
repository.name.fl_str_mv |
Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
repositorio@ufmg.br |
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1816829909353365504 |