Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes

Detalhes bibliográficos
Autor(a) principal: Michele Munk Pereira
Data de Publicação: 2016
Outros Autores: Ado Jorio de Vasconcelos, Humberto de Mello Brandão, Luiz Orlando Ladeira, Bruno Campos de Carvalho, Luiz Sérgio de Almeida Camargo, Nádia Rezende Barbosa Raposo, Raquel Varella Serapião, Carolina Capobiango Romano Quintão, Saulo Rodrigues Silva, Jaqueline dos Santos Soares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: http://hdl.handle.net/1843/41917
https://orcid.org/0000-0001-8927-6190
https://orcid.org/0000-0002-5978-2735
https://orcid.org/0000-0002-7405-3928
https://orcid.org/0000-0002-4935-6070
https://orcid.org/0000-0001-9974-711X
https://orcid.org/0000-0001-5271-1048
https://orcid.org/0000-0002-4198-5753
https://orcid.org/0000-0002-6232-2174
Resumo: The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.
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spelling Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubesNanotubesPelucid zoneTransfectionDNANanotubosDNAThe pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal de Minas GeraisBrasilICX - DEPARTAMENTO DE FÍSICAUFMG2022-05-24T12:18:01Z2022-05-24T12:18:01Z2016-09-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf10.1038/srep335882045-2322http://hdl.handle.net/1843/41917https://orcid.org/0000-0001-8927-6190https://orcid.org/0000-0002-5978-2735https://orcid.org/0000-0002-7405-3928https://orcid.org/0000-0002-4935-6070https://orcid.org/0000-0001-9974-711Xhttps://orcid.org/0000-0001-5271-1048https://orcid.org/0000-0002-4198-5753https://orcid.org/0000-0002-6232-2174engScientific ReportsMichele Munk PereiraAdo Jorio de VasconcelosHumberto de Mello BrandãoLuiz Orlando LadeiraBruno Campos de CarvalhoLuiz Sérgio de Almeida CamargoNádia Rezende Barbosa RaposoRaquel Varella SerapiãoCarolina Capobiango Romano QuintãoSaulo Rodrigues SilvaJaqueline dos Santos Soaresinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2022-05-24T12:18:02Zoai:repositorio.ufmg.br:1843/41917Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2022-05-24T12:18:02Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
title Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
spellingShingle Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
Michele Munk Pereira
Nanotubes
Pelucid zone
Transfection
DNA
Nanotubos
DNA
title_short Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
title_full Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
title_fullStr Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
title_full_unstemmed Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
title_sort Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
author Michele Munk Pereira
author_facet Michele Munk Pereira
Ado Jorio de Vasconcelos
Humberto de Mello Brandão
Luiz Orlando Ladeira
Bruno Campos de Carvalho
Luiz Sérgio de Almeida Camargo
Nádia Rezende Barbosa Raposo
Raquel Varella Serapião
Carolina Capobiango Romano Quintão
Saulo Rodrigues Silva
Jaqueline dos Santos Soares
author_role author
author2 Ado Jorio de Vasconcelos
Humberto de Mello Brandão
Luiz Orlando Ladeira
Bruno Campos de Carvalho
Luiz Sérgio de Almeida Camargo
Nádia Rezende Barbosa Raposo
Raquel Varella Serapião
Carolina Capobiango Romano Quintão
Saulo Rodrigues Silva
Jaqueline dos Santos Soares
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Michele Munk Pereira
Ado Jorio de Vasconcelos
Humberto de Mello Brandão
Luiz Orlando Ladeira
Bruno Campos de Carvalho
Luiz Sérgio de Almeida Camargo
Nádia Rezende Barbosa Raposo
Raquel Varella Serapião
Carolina Capobiango Romano Quintão
Saulo Rodrigues Silva
Jaqueline dos Santos Soares
dc.subject.por.fl_str_mv Nanotubes
Pelucid zone
Transfection
DNA
Nanotubos
DNA
topic Nanotubes
Pelucid zone
Transfection
DNA
Nanotubos
DNA
description The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-19
2022-05-24T12:18:01Z
2022-05-24T12:18:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 10.1038/srep33588
2045-2322
http://hdl.handle.net/1843/41917
https://orcid.org/0000-0001-8927-6190
https://orcid.org/0000-0002-5978-2735
https://orcid.org/0000-0002-7405-3928
https://orcid.org/0000-0002-4935-6070
https://orcid.org/0000-0001-9974-711X
https://orcid.org/0000-0001-5271-1048
https://orcid.org/0000-0002-4198-5753
https://orcid.org/0000-0002-6232-2174
identifier_str_mv 10.1038/srep33588
2045-2322
url http://hdl.handle.net/1843/41917
https://orcid.org/0000-0001-8927-6190
https://orcid.org/0000-0002-5978-2735
https://orcid.org/0000-0002-7405-3928
https://orcid.org/0000-0002-4935-6070
https://orcid.org/0000-0001-9974-711X
https://orcid.org/0000-0001-5271-1048
https://orcid.org/0000-0002-4198-5753
https://orcid.org/0000-0002-6232-2174
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Scientific Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
Brasil
ICX - DEPARTAMENTO DE FÍSICA
UFMG
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
Brasil
ICX - DEPARTAMENTO DE FÍSICA
UFMG
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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