Chemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.5897/JMPR2017.6343 http://hdl.handle.net/1843/59345 https://orcid.org/0000-0002-8831-1609 |
Resumo: | The content and chemical composition of the essential oil may vary in certain species according to the climatic period. The aim of this study was to evaluate the influence of seasonality on the chemical composition of the essential oil from Hyptis dilatata flowers, to perform biological activities such as antimicrobial, inhibition of acetylcholinesterase enzyme and to evaluate the toxicity of the essential oil using for the test as indicator Artemia salina. H. dilatata flowers were collected in rainy and dry periods and extracted by hydro distillation using extractor of Clevenger condenser double Spell model. Analysis of essential oil resulted in 22 chemical components. The major constituents for dry and rainy periods were α-pinene (26.2 and 10.9%), 3-Carene (12.2 and 3.7%), fenchone (17% and 14.8%) and β-cariophyllene (16.36 and 30.9%), respectively. The essential oil inhibited the acetylcholinesterase enzyme in 93.4% (rainy period) and 92.4% (dry period). Between the dry and rainy periods, the best LC50 in microbial activity in vitro was obtained in the rainy period tested in Staphylococcus aureus bacterium (LC50 49.8 mg ml-1). The cytotoxic activity of A. salina in H. dilatata essential oil proved LC50 results below of 100 μg ml-1. Therefore, the chemical characterization and testing of biological activities of essential oils showed promising results in the search for new active substances and development of bioproducts of vegetable origin. |
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2023-10-09T23:20:42Z2023-10-09T23:20:42Z20171114275283https://doi.org/10.5897/JMPR2017.63431996-0875http://hdl.handle.net/1843/59345https://orcid.org/0000-0002-8831-1609The content and chemical composition of the essential oil may vary in certain species according to the climatic period. The aim of this study was to evaluate the influence of seasonality on the chemical composition of the essential oil from Hyptis dilatata flowers, to perform biological activities such as antimicrobial, inhibition of acetylcholinesterase enzyme and to evaluate the toxicity of the essential oil using for the test as indicator Artemia salina. H. dilatata flowers were collected in rainy and dry periods and extracted by hydro distillation using extractor of Clevenger condenser double Spell model. Analysis of essential oil resulted in 22 chemical components. The major constituents for dry and rainy periods were α-pinene (26.2 and 10.9%), 3-Carene (12.2 and 3.7%), fenchone (17% and 14.8%) and β-cariophyllene (16.36 and 30.9%), respectively. The essential oil inhibited the acetylcholinesterase enzyme in 93.4% (rainy period) and 92.4% (dry period). Between the dry and rainy periods, the best LC50 in microbial activity in vitro was obtained in the rainy period tested in Staphylococcus aureus bacterium (LC50 49.8 mg ml-1). The cytotoxic activity of A. salina in H. dilatata essential oil proved LC50 results below of 100 μg ml-1. Therefore, the chemical characterization and testing of biological activities of essential oils showed promising results in the search for new active substances and development of bioproducts of vegetable origin.engUniversidade Federal de Minas GeraisUFMGBrasilICX - DEPARTAMENTO DE QUÍMICAJournal of Medicinal Plants ResearchQuímicaEssências e óleos essenciaisHyptis dilatataα-pineneFenchoneβ-cariofilene3-careneChemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://academicjournals.org/journal/JMPR/article-abstract/A4C830663757Sirley Pereira AlmeidaPedro Tadei WanderliDiana Maruja Sangama MozombiteAntonio Alves de Melo FilhoAndreina G. de Andrade LaranjeiraRegildo M. G. MartinsJacqueline Aparecida TakahashiVany P. FerrazRibeiro Duarte Otonielapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/59345/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALChemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers.pdfChemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers.pdfapplication/pdf443409https://repositorio.ufmg.br/bitstream/1843/59345/2/Chemical%20composition%2c%20antiacetylcholinesterase%20%20inhibition%20and%20toxicity%20activities%20of%20essential%20oil%20from%20%20Hyptis%20dilatata%20%28Lamiaceae%29%20flowers.pdfcd7f408c4891cd7d8dd9cb9b810bf8f9MD521843/593452023-10-09 20:20:42.982oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-10-09T23:20:42Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Chemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers |
title |
Chemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers |
spellingShingle |
Chemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers Sirley Pereira Almeida Hyptis dilatata α-pinene Fenchone β-cariofilene 3-carene Química Essências e óleos essenciais |
title_short |
Chemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers |
title_full |
Chemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers |
title_fullStr |
Chemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers |
title_full_unstemmed |
Chemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers |
title_sort |
Chemical composition, antiacetylcholinesterase inhibition and toxicity activities of essential oil from Hyptis dilatata (Lamiaceae) flowers |
author |
Sirley Pereira Almeida |
author_facet |
Sirley Pereira Almeida Pedro Tadei Wanderli Diana Maruja Sangama Mozombite Antonio Alves de Melo Filho Andreina G. de Andrade Laranjeira Regildo M. G. Martins Jacqueline Aparecida Takahashi Vany P. Ferraz Ribeiro Duarte Otoniel |
author_role |
author |
author2 |
Pedro Tadei Wanderli Diana Maruja Sangama Mozombite Antonio Alves de Melo Filho Andreina G. de Andrade Laranjeira Regildo M. G. Martins Jacqueline Aparecida Takahashi Vany P. Ferraz Ribeiro Duarte Otoniel |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sirley Pereira Almeida Pedro Tadei Wanderli Diana Maruja Sangama Mozombite Antonio Alves de Melo Filho Andreina G. de Andrade Laranjeira Regildo M. G. Martins Jacqueline Aparecida Takahashi Vany P. Ferraz Ribeiro Duarte Otoniel |
dc.subject.por.fl_str_mv |
Hyptis dilatata α-pinene Fenchone β-cariofilene 3-carene |
topic |
Hyptis dilatata α-pinene Fenchone β-cariofilene 3-carene Química Essências e óleos essenciais |
dc.subject.other.pt_BR.fl_str_mv |
Química Essências e óleos essenciais |
description |
The content and chemical composition of the essential oil may vary in certain species according to the climatic period. The aim of this study was to evaluate the influence of seasonality on the chemical composition of the essential oil from Hyptis dilatata flowers, to perform biological activities such as antimicrobial, inhibition of acetylcholinesterase enzyme and to evaluate the toxicity of the essential oil using for the test as indicator Artemia salina. H. dilatata flowers were collected in rainy and dry periods and extracted by hydro distillation using extractor of Clevenger condenser double Spell model. Analysis of essential oil resulted in 22 chemical components. The major constituents for dry and rainy periods were α-pinene (26.2 and 10.9%), 3-Carene (12.2 and 3.7%), fenchone (17% and 14.8%) and β-cariophyllene (16.36 and 30.9%), respectively. The essential oil inhibited the acetylcholinesterase enzyme in 93.4% (rainy period) and 92.4% (dry period). Between the dry and rainy periods, the best LC50 in microbial activity in vitro was obtained in the rainy period tested in Staphylococcus aureus bacterium (LC50 49.8 mg ml-1). The cytotoxic activity of A. salina in H. dilatata essential oil proved LC50 results below of 100 μg ml-1. Therefore, the chemical characterization and testing of biological activities of essential oils showed promising results in the search for new active substances and development of bioproducts of vegetable origin. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2023-10-09T23:20:42Z |
dc.date.available.fl_str_mv |
2023-10-09T23:20:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/59345 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.5897/JMPR2017.6343 |
dc.identifier.issn.pt_BR.fl_str_mv |
1996-0875 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-8831-1609 |
url |
https://doi.org/10.5897/JMPR2017.6343 http://hdl.handle.net/1843/59345 https://orcid.org/0000-0002-8831-1609 |
identifier_str_mv |
1996-0875 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Journal of Medicinal Plants Research |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICX - DEPARTAMENTO DE QUÍMICA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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Universidade Federal de Minas Gerais (UFMG) |
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UFMG |
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UFMG |
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