Comparative genomics and in silico evaluation of genes related to probiotic potential of Bifidobacterium breve 1101a

• Autor(a) principal: Juan Luis Valdez Baez
• Data de Publicação: 2021
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: http://hdl.handle.net/1843/38128
• topic: Pangenomic analyses; Comparative genomics; Probiotic; Bifidobacterium breve; Biologia computacional; Genômica; Probióticos; Bifidobacterium

Probiotics are microorganisms with the ability to influence the composition of the intestinal microbiota and promote human health. The role of microorganisms in the human microbiota and on health has increased in importance in the last years and more approaches are used to elucidate their properties. Genomics and the bioinformatic analysis of available bacterial data have been used in the main genera of probiotics Lactobacillus e Bifidobacterium. Bifidobacterium breve is considered a safe species, dominant in newborns and it is used in probiotic products. B. breve to treat necrotizing enterocolitis (NEC), gastrointestinal disorders, celiac disease, pediatric obesity, and allergies in infants with positive results. In this context, the present study aims to perform the in silico characterization of the genome of Bifidobacterium breve 1101A strain through a comparative genomic analysis and the identification of genes related to probiotic features. For this purpose, it was employed additional 45 available complete genomes of Bifidobacterium breve to (i) analyze the taxonomic and phylogenomic aspects of this genus, (ii) identify mobile elements in the 1101A genome such as prophages, plasmids, IS, (iii) genomic islands (GEI), antibiotic resistance genes and (iv) analyze the pangenome. Between the results, the strain 1101A was identified as Bifidobacterium breve and the phylogenetically closest strain was B. breve NRBB26. An incomplete prophage was predicted in B. breve 1101A genome, without plasmids. Moreover, seven genomic islands (GEI) were identified: two Resistance Islands (RI) and five Genomic Islands (GI). Resistance genes present in the genome were rpoB, iles and ermX. The pangenome size was calculated in 5943 genes and the core genome in 1174 genes and it was considered an open pangenome according to previous studies. There were 63 unique genes related to the metabolism of carbohydrates, such as galactosidase and DNA binding. Also, some genes related to adherence, resistance to stress, repair and protection of DNA and proteins, production of vitamins was identified. These results reveal the probiotic potential of this bacterial strain and direct further studies in vitro and in vivo to confirm its properties.