Inactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment (aPDT)

Detalhes bibliográficos
Autor(a) principal: Leonardo do Prado-Silva
Data de Publicação: 2021
Outros Autores: Verônica Ortiz Alvarenga, Gilberto Úbida Leite Braga, Anderson de Souza Sant'Ana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1016/j.lwt.2021.111037
http://hdl.handle.net/1843/51500
Resumo: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
id UFMG_11ae27305819703e747b183f867e226b
oai_identifier_str oai:repositorio.ufmg.br:1843/51500
network_acronym_str UFMG
network_name_str Repositório Institucional da UFMG
repository_id_str
spelling 2023-04-03T19:43:29Z2023-04-03T19:43:29Z2021-0514217https://doi.org/10.1016/j.lwt.2021.1110370023-6438http://hdl.handle.net/1843/51500CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorAntimicrobial photodynamic treatment (aPDT) is a promising alternative to conventional thermal inactivation methods. This study aimed to evaluate Bacillus cereus vegetative cells' inactivation and spores by aPDT with new methylene blue (NMB) as photosensitizer (PS) using red light. The efficacy of aPDT was determined initially by minimal inhibitory concentration (MIC) of NMB at different concentrations and fluences. Cluster analysis from the results of MIC grouped B. cereus strains according to their aPDT susceptibility. Then, four strains (B63, B3, 436, and ATCC 14579) were selected for a survival study of aPDT with NMB in three concentrations (5, 50, and 100 μM). The viability of B. cereus vegetative cells and spores was reduced in all tested fluences. The strain ATCC 14579 (vegetative cells and spores) was the most susceptible to aPDT at relatively low fluence (25 J/cm2). The Weibull model presented a good fit for the inactivation data estimating the kinetic parameters δ (first decimal reduction) and p (shape parameter). This study contributes to the knowledge of the behavior of different strains of B. cereus regarding an emerging method of microbial inactivation. The variability of inactivation among strains will allow the development of more reliable processes.engUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ALIMENTOSLWTTratamento fotodinâmico antimicrobianoCélulas vegetativasEsporos de Bacillus cereusPhenothiazine dyeSpoilagePathogenNonthermal treatmentEmerging technologyInactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment (aPDT)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S0023643821001900Leonardo do Prado-SilvaVerônica Ortiz AlvarengaGilberto Úbida Leite BragaAnderson de Souza Sant'Anaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/51500/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALInactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment aPDT.pdfInactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment aPDT.pdfapplication/pdf2143651https://repositorio.ufmg.br/bitstream/1843/51500/2/Inactivation%20kinetics%20of%20Bacillus%20cereus%20vegetative%20cells%20and%20spores%20from%20different%20sources%20by%20antimicrobial%20photodynamic%20treatment%20aPDT.pdfa112dcb3af384cc71ac2352900fd9c9dMD521843/515002023-04-03 16:47:57.464oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-04-03T19:47:57Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Inactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment (aPDT)
title Inactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment (aPDT)
spellingShingle Inactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment (aPDT)
Leonardo do Prado-Silva
Phenothiazine dye
Spoilage
Pathogen
Nonthermal treatment
Emerging technology
Tratamento fotodinâmico antimicrobiano
Células vegetativas
Esporos de Bacillus cereus
title_short Inactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment (aPDT)
title_full Inactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment (aPDT)
title_fullStr Inactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment (aPDT)
title_full_unstemmed Inactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment (aPDT)
title_sort Inactivation kinetics of Bacillus cereus vegetative cells and spores from different sources by antimicrobial photodynamic treatment (aPDT)
author Leonardo do Prado-Silva
author_facet Leonardo do Prado-Silva
Verônica Ortiz Alvarenga
Gilberto Úbida Leite Braga
Anderson de Souza Sant'Ana
author_role author
author2 Verônica Ortiz Alvarenga
Gilberto Úbida Leite Braga
Anderson de Souza Sant'Ana
author2_role author
author
author
dc.contributor.author.fl_str_mv Leonardo do Prado-Silva
Verônica Ortiz Alvarenga
Gilberto Úbida Leite Braga
Anderson de Souza Sant'Ana
dc.subject.por.fl_str_mv Phenothiazine dye
Spoilage
Pathogen
Nonthermal treatment
Emerging technology
topic Phenothiazine dye
Spoilage
Pathogen
Nonthermal treatment
Emerging technology
Tratamento fotodinâmico antimicrobiano
Células vegetativas
Esporos de Bacillus cereus
dc.subject.other.pt_BR.fl_str_mv Tratamento fotodinâmico antimicrobiano
Células vegetativas
Esporos de Bacillus cereus
description CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
publishDate 2021
dc.date.issued.fl_str_mv 2021-05
dc.date.accessioned.fl_str_mv 2023-04-03T19:43:29Z
dc.date.available.fl_str_mv 2023-04-03T19:43:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/51500
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1016/j.lwt.2021.111037
dc.identifier.issn.pt_BR.fl_str_mv 0023-6438
url https://doi.org/10.1016/j.lwt.2021.111037
http://hdl.handle.net/1843/51500
identifier_str_mv 0023-6438
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv LWT
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv FAR - DEPARTAMENTO DE ALIMENTOS
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
bitstream.url.fl_str_mv https://repositorio.ufmg.br/bitstream/1843/51500/1/License.txt
https://repositorio.ufmg.br/bitstream/1843/51500/2/Inactivation%20kinetics%20of%20Bacillus%20cereus%20vegetative%20cells%20and%20spores%20from%20different%20sources%20by%20antimicrobial%20photodynamic%20treatment%20aPDT.pdf
bitstream.checksum.fl_str_mv fa505098d172de0bc8864fc1287ffe22
a112dcb3af384cc71ac2352900fd9c9d
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv
_version_ 1803589413218811904

Registros relacionados