Bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/39484 |
Resumo: | Several products of industrial interest are produced by Bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. This study aimed to investigate the potential of two bacterial isolates (P5 and C3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. Phylogenetic analyses showed close relation of isolates P5 and C3 with Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively. Notably, Bacillus sp. P5 showed antimicrobial activity against pathogens such as Listeria monocytogenes and Bacillus cereus, in addition to antifungal activity. The presence of genes encoding pre-subtilosin (sboA), malonyl CoA transacylase (ituD), and the putative transcriptional terminator of surfactin (sfp) were detected in Bacillus sp. P5, suggesting the production of the bacteriocin subtilosin A and the lipopeptides iturin A and surfactin by this strain. For Bacillus sp. C3 the presence of sboA and spas (subtilin) genes was observed by the first time in members of B. cereus cluster. Bacillus sp. P5 showed emulsifying capability on mineral oil, soybean biodiesel and toluene, while Bacillus sp. C3 showed emulsifying capability only on mineral oil. The reduction of the surface tension in culture medium was also observed for strain P5, confirming the production of surface-active compounds by this bacterium. Monoprotonated molecular species and adducts of sodium and potassium ions of surfactin, iturin, and fengycin were detected in the P5 culture medium. Comparative MS/MS spectra of the peak m/z 1030 (C14 surfactin A or C15 surfactin B [M+Na]+) and peak m/z 1079 (C15 iturin [M+Na]+) showed the same fragmentation profile of standards, confirming the molecular identification. In conclusion, Bacillus sp. P5 showed the best potential for the production of antifungal, antibacterial, and biosurfactant substances. |
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2022-02-18T21:37:41Z2022-02-18T21:37:41Z2017-01-3186111410.3389/fmicb.2017.000611664302Xhttp://hdl.handle.net/1843/39484Several products of industrial interest are produced by Bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. This study aimed to investigate the potential of two bacterial isolates (P5 and C3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. Phylogenetic analyses showed close relation of isolates P5 and C3 with Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively. Notably, Bacillus sp. P5 showed antimicrobial activity against pathogens such as Listeria monocytogenes and Bacillus cereus, in addition to antifungal activity. The presence of genes encoding pre-subtilosin (sboA), malonyl CoA transacylase (ituD), and the putative transcriptional terminator of surfactin (sfp) were detected in Bacillus sp. P5, suggesting the production of the bacteriocin subtilosin A and the lipopeptides iturin A and surfactin by this strain. For Bacillus sp. C3 the presence of sboA and spas (subtilin) genes was observed by the first time in members of B. cereus cluster. Bacillus sp. P5 showed emulsifying capability on mineral oil, soybean biodiesel and toluene, while Bacillus sp. C3 showed emulsifying capability only on mineral oil. The reduction of the surface tension in culture medium was also observed for strain P5, confirming the production of surface-active compounds by this bacterium. Monoprotonated molecular species and adducts of sodium and potassium ions of surfactin, iturin, and fengycin were detected in the P5 culture medium. Comparative MS/MS spectra of the peak m/z 1030 (C14 surfactin A or C15 surfactin B [M+Na]+) and peak m/z 1079 (C15 iturin [M+Na]+) showed the same fragmentation profile of standards, confirming the molecular identification. In conclusion, Bacillus sp. P5 showed the best potential for the production of antifungal, antibacterial, and biosurfactant substances.Vários produtos de interesse industrial são produzidos pela Bacillus, incluindo enzimas, antibióticos, aminoácidos, inseticidas, biossurfactantes e bacteriocinas. Este estudo teve como objetivo investigar o potencial de dois isolados bacterianos (P5 e C3) de puba, produto da fermentação regional da mandioca, para produzir múltiplas substâncias com propriedades antimicrobianas e tensoativas. As análises filogenéticas mostraram estreita relação dos isolados P5 e C3 com Bacillus amyloliquefaciens e Bacillus thuringiensis, respectivamente. Notavelmente, Bacillus sp. P5 apresentou atividade antimicrobiana contra patógenos como Listeria monocytogenes e Bacillus cereus, além de atividade antifúngica. A presença de genes que codificam pré-subtilosina (sboA), malonil CoA transacilase (ituD) e o suposto terminador transcricional de surfactina (sfp) foram detectados em Bacillus sp. P5, sugerindo a produção da bacteriocina subtilosina A e dos lipopeptídeos iturina A e surfactina por esta cepa. Para Bacillus sp. C3 a presença dos genes sboA e spas (subtilina) foi observada pela primeira vez em membros do cluster B. cereus. Bacilo sp. P5 apresentou capacidade emulsificante em óleo mineral, biodiesel de soja e tolueno, enquanto Bacillus sp. C3 apresentou capacidade emulsificante apenas em óleo mineral. A redução da tensão superficial em meio de cultura também foi observada para a cepa P5, confirmando a produção de compostos tensoativos por esta bactéria. Espécies moleculares monoprotonadas e adutos de íons sódio e potássio de surfactina, iturina e fengicina foram detectados no meio de cultura P5. Espectros MS/MS comparativos do pico m/z 1030 (C14 surfactina A ou C15 surfactina B [M+Na]+) e pico m/z 1079 (C15 iturina [M+Na]+) mostraram o mesmo perfil de fragmentação dos padrões , confirmando a identificação molecular. Em conclusão, Bacillus sp. O P5 apresentou o melhor potencial para a produção de substâncias antifúngicas, antibacterianas e biossurfactantes.engUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE MICROBIOLOGIAFrontiers in microbiologyBioquímicaMicrobiologiaAntimicrobial peptidesBiosurfactantFermented foodBacillus sppCassavaMALDI-TOF mass spectrometryBacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptidesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articledoi:10.3389/fmicb.2017.00061Karla J. PerezAdriano BrandelliRegina M. D. NardiJaime Dos Santos VianaFernanda C. LopesJamile Q. PereiraDaniel M. Dos SantosJamil Silvano de OliveiraRenata V. VelhoSilvia M. CrispimJacques R. 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dc.title.pt_BR.fl_str_mv |
Bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides |
title |
Bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides |
spellingShingle |
Bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides Karla J. Perez Antimicrobial peptides Biosurfactant Fermented food Bacillus spp Cassava MALDI-TOF mass spectrometry Bioquímica Microbiologia |
title_short |
Bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides |
title_full |
Bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides |
title_fullStr |
Bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides |
title_full_unstemmed |
Bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides |
title_sort |
Bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides |
author |
Karla J. Perez |
author_facet |
Karla J. Perez Adriano Brandelli Regina M. D. Nardi Jaime Dos Santos Viana Fernanda C. Lopes Jamile Q. Pereira Daniel M. Dos Santos Jamil Silvano de Oliveira Renata V. Velho Silvia M. Crispim Jacques R. Nicoli |
author_role |
author |
author2 |
Adriano Brandelli Regina M. D. Nardi Jaime Dos Santos Viana Fernanda C. Lopes Jamile Q. Pereira Daniel M. Dos Santos Jamil Silvano de Oliveira Renata V. Velho Silvia M. Crispim Jacques R. Nicoli |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Karla J. Perez Adriano Brandelli Regina M. D. Nardi Jaime Dos Santos Viana Fernanda C. Lopes Jamile Q. Pereira Daniel M. Dos Santos Jamil Silvano de Oliveira Renata V. Velho Silvia M. Crispim Jacques R. Nicoli |
dc.subject.por.fl_str_mv |
Antimicrobial peptides Biosurfactant Fermented food Bacillus spp Cassava MALDI-TOF mass spectrometry |
topic |
Antimicrobial peptides Biosurfactant Fermented food Bacillus spp Cassava MALDI-TOF mass spectrometry Bioquímica Microbiologia |
dc.subject.other.pt_BR.fl_str_mv |
Bioquímica Microbiologia |
description |
Several products of industrial interest are produced by Bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. This study aimed to investigate the potential of two bacterial isolates (P5 and C3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. Phylogenetic analyses showed close relation of isolates P5 and C3 with Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively. Notably, Bacillus sp. P5 showed antimicrobial activity against pathogens such as Listeria monocytogenes and Bacillus cereus, in addition to antifungal activity. The presence of genes encoding pre-subtilosin (sboA), malonyl CoA transacylase (ituD), and the putative transcriptional terminator of surfactin (sfp) were detected in Bacillus sp. P5, suggesting the production of the bacteriocin subtilosin A and the lipopeptides iturin A and surfactin by this strain. For Bacillus sp. C3 the presence of sboA and spas (subtilin) genes was observed by the first time in members of B. cereus cluster. Bacillus sp. P5 showed emulsifying capability on mineral oil, soybean biodiesel and toluene, while Bacillus sp. C3 showed emulsifying capability only on mineral oil. The reduction of the surface tension in culture medium was also observed for strain P5, confirming the production of surface-active compounds by this bacterium. Monoprotonated molecular species and adducts of sodium and potassium ions of surfactin, iturin, and fengycin were detected in the P5 culture medium. Comparative MS/MS spectra of the peak m/z 1030 (C14 surfactin A or C15 surfactin B [M+Na]+) and peak m/z 1079 (C15 iturin [M+Na]+) showed the same fragmentation profile of standards, confirming the molecular identification. In conclusion, Bacillus sp. P5 showed the best potential for the production of antifungal, antibacterial, and biosurfactant substances. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-01-31 |
dc.date.accessioned.fl_str_mv |
2022-02-18T21:37:41Z |
dc.date.available.fl_str_mv |
2022-02-18T21:37:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/39484 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.3389/fmicb.2017.00061 |
dc.identifier.issn.pt_BR.fl_str_mv |
1664302X |
identifier_str_mv |
10.3389/fmicb.2017.00061 1664302X |
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http://hdl.handle.net/1843/39484 |
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eng |
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eng |
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Frontiers in microbiology |
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openAccess |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - DEPARTAMENTO DE MICROBIOLOGIA |
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Universidade Federal de Minas Gerais |
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UFMG |
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