The Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependent
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/57579 https://orcid.org/0000-0003-3089-655X |
Resumo: | There is a large increase in the number of elderly people in modern societies. This demographic phenomenon has been paralleled by an epidemic of chronic diseases and inflammatory process usually associated with advanced age. In this work we seek to fill a gap that still exists in the process of immunoregulation “old guy’ looking to narrow the knowledge regarding their correlation with the immune system and the nervous system. The Nitric Oxide (NO) and Interleukins productions were studied in healthy subjects in age ranging from 20 to 80 years divided in three age groups: (20-39), (40-59) and (60-80) years. NO production was quantified in a Griess reaction. The results were expressed through the nitrite concentration calculated by linear regression using a standard curve obtained from a solution of sodium nitrite and 1mM RPMI. Interleukins 8, 10 and 4 (IL-8, IL-10 and IL-4) productions were measured by sandwich ELISA. Ours results demonstrated that Norepinephrine (NoAD) can no longer modulate the inflammatory cytokine profile and that there is a significant decay of the contribution of Caffeic Acid (CA) as an antioxidant and / or anti-inflammatory age-related. Thus, our results showed a lack of NoAD and CA modulation in human granulocyte in ageing dependent process. |
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2023-08-07T20:41:53Z2023-08-07T20:41:53Z2018-01-2321172002-7850http://hdl.handle.net/1843/57579https://orcid.org/0000-0003-3089-655XThere is a large increase in the number of elderly people in modern societies. This demographic phenomenon has been paralleled by an epidemic of chronic diseases and inflammatory process usually associated with advanced age. In this work we seek to fill a gap that still exists in the process of immunoregulation “old guy’ looking to narrow the knowledge regarding their correlation with the immune system and the nervous system. The Nitric Oxide (NO) and Interleukins productions were studied in healthy subjects in age ranging from 20 to 80 years divided in three age groups: (20-39), (40-59) and (60-80) years. NO production was quantified in a Griess reaction. The results were expressed through the nitrite concentration calculated by linear regression using a standard curve obtained from a solution of sodium nitrite and 1mM RPMI. Interleukins 8, 10 and 4 (IL-8, IL-10 and IL-4) productions were measured by sandwich ELISA. Ours results demonstrated that Norepinephrine (NoAD) can no longer modulate the inflammatory cytokine profile and that there is a significant decay of the contribution of Caffeic Acid (CA) as an antioxidant and / or anti-inflammatory age-related. Thus, our results showed a lack of NoAD and CA modulation in human granulocyte in ageing dependent process.engUniversidade Federal de Minas GeraisUFMGBrasilMED - DEPARTAMENTO DE CLÍNICA MÉDICAAgeing Science & Mental Health StudiesEnvelhecimentoGranulócitosÁcidos CafeicosCitocinasAgingGranulocyteCaffeic acidsNitric oxidCytokinesThe Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://researchopenworld.com/the-effect-of-norepinephrine-and-caffeic-acid-on-cytokines-and-nitric-oxide-production-by-granulocytes-is-age-dependent/Raquel C. LaraRodrigo P. LaraC. S. BedettiE. P. Cunha-AzevedoMarco Túlio Gualberto CintraEdgar Nunes de MoraesJosé Augusto Nogueira-MachadoMiriam Martins Chavesapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/57579/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALThe Effect of Norepinephrine and Caffeic Acid on Cytokines and pdfa.pdfThe Effect of Norepinephrine and Caffeic Acid on Cytokines and pdfa.pdfapplication/pdf667742https://repositorio.ufmg.br/bitstream/1843/57579/2/The%20Effect%20of%20Norepinephrine%20and%20Caffeic%20Acid%20on%20Cytokines%20and%20pdfa.pdfe814bf2120f28bc4d62437434e451ff5MD521843/575792023-08-07 17:58:23.781oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-08-07T20:58:23Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
The Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependent |
title |
The Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependent |
spellingShingle |
The Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependent Raquel C. Lara Aging Granulocyte Caffeic acids Nitric oxid Cytokines Envelhecimento Granulócitos Ácidos Cafeicos Citocinas |
title_short |
The Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependent |
title_full |
The Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependent |
title_fullStr |
The Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependent |
title_full_unstemmed |
The Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependent |
title_sort |
The Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependent |
author |
Raquel C. Lara |
author_facet |
Raquel C. Lara Rodrigo P. Lara C. S. Bedetti E. P. Cunha-Azevedo Marco Túlio Gualberto Cintra Edgar Nunes de Moraes José Augusto Nogueira-Machado Miriam Martins Chaves |
author_role |
author |
author2 |
Rodrigo P. Lara C. S. Bedetti E. P. Cunha-Azevedo Marco Túlio Gualberto Cintra Edgar Nunes de Moraes José Augusto Nogueira-Machado Miriam Martins Chaves |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Raquel C. Lara Rodrigo P. Lara C. S. Bedetti E. P. Cunha-Azevedo Marco Túlio Gualberto Cintra Edgar Nunes de Moraes José Augusto Nogueira-Machado Miriam Martins Chaves |
dc.subject.por.fl_str_mv |
Aging Granulocyte Caffeic acids Nitric oxid Cytokines |
topic |
Aging Granulocyte Caffeic acids Nitric oxid Cytokines Envelhecimento Granulócitos Ácidos Cafeicos Citocinas |
dc.subject.other.pt_BR.fl_str_mv |
Envelhecimento Granulócitos Ácidos Cafeicos Citocinas |
description |
There is a large increase in the number of elderly people in modern societies. This demographic phenomenon has been paralleled by an epidemic of chronic diseases and inflammatory process usually associated with advanced age. In this work we seek to fill a gap that still exists in the process of immunoregulation “old guy’ looking to narrow the knowledge regarding their correlation with the immune system and the nervous system. The Nitric Oxide (NO) and Interleukins productions were studied in healthy subjects in age ranging from 20 to 80 years divided in three age groups: (20-39), (40-59) and (60-80) years. NO production was quantified in a Griess reaction. The results were expressed through the nitrite concentration calculated by linear regression using a standard curve obtained from a solution of sodium nitrite and 1mM RPMI. Interleukins 8, 10 and 4 (IL-8, IL-10 and IL-4) productions were measured by sandwich ELISA. Ours results demonstrated that Norepinephrine (NoAD) can no longer modulate the inflammatory cytokine profile and that there is a significant decay of the contribution of Caffeic Acid (CA) as an antioxidant and / or anti-inflammatory age-related. Thus, our results showed a lack of NoAD and CA modulation in human granulocyte in ageing dependent process. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-01-23 |
dc.date.accessioned.fl_str_mv |
2023-08-07T20:41:53Z |
dc.date.available.fl_str_mv |
2023-08-07T20:41:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/57579 |
dc.identifier.issn.pt_BR.fl_str_mv |
2002-7850 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0003-3089-655X |
identifier_str_mv |
2002-7850 |
url |
http://hdl.handle.net/1843/57579 https://orcid.org/0000-0003-3089-655X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Ageing Science & Mental Health Studies |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
MED - DEPARTAMENTO DE CLÍNICA MÉDICA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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