| spelling |
Walter dos Santos Limahttp://lattes.cnpq.br/8855532073043045Joziana Muniz de Paiva BarçanteMônica Alves Neves Diniz FerreiraVitor Márcio Ribeirohttp://lattes.cnpq.br/3177322809082851Isabela Resende Ávila2021-03-25T15:25:17Z2021-03-25T15:25:17Z2019-02-26http://hdl.handle.net/1843/35402Angiostrongylus vasorum é um nematoide, cujas formas adultas podem ser encontradas nas artérias pulmonares e coração direito de cães domésticos e canídeos silvestres, que são os hospedeiros definitivos. Além da importância na clínica de pequenos animais, este parasito tem importância discutida em termos de saúde pública, devido à possibilidade de infecção humana. O ciclo heteroxeno, apresenta diversas espécies de moluscos como hospedeiros intermediários, que se infectam por ingestão e/ou penetração de larvas de primeiro estádio do parasito. Nos moluscos ocorrem duas mudas, de larva de primeiro estádio (L1) para L2 e L3, que é infectante para os hospedeiros definitivos. Além dessas vias de infecção, pode haver também a participação de hospedeiros paratênicos, que podem abrigar as L3, como por exemplo, rãs, camundongos, ratos e aves. Considerando que várias questões inerentes ao ciclo deste parasito ainda permanecem obscuras, o presente estudo teve como objetivo avaliar se Biomphalaria glabrata pode ser hospedeiro paratênico de Angiostrongylus vasorum, identificando as possíveis vias de penetração destas larvas, a rota migratória, além de descrever as possíveis alterações histológicas em diferentes tempos de infecção. Larvas de terceiro estádio foram recuperadas de moluscos da espécie B. glabrata, com 30 dias após infecção com 1.000 L1 de A. vasorum, utilizando o método de Baermann modificado. As L3 recuperadas foram utilizadas para infecção de moluscos da espécie B. glabrata, com o número de 100 L3+ (Larva de terceiro estádio advinda de molusco infectado com 100 L3). Larvas de terceiro estádio de A. vasorum foram recuperadas vivas e ativas nos tecidos dos moluscos, demonstrando assim, a permissividade destes a infecção por L3+. Os demais exemplares de moluscos infectados, foram fixados em formol 10% para avaliação histológica, onde se observou a presença de larva no tecido e que as mesmas tendem a ficar na região cefalopodal. Em relação a viabilidade das L3+, ainda são necessários mais estudos, visto que dois cães sem raça definida foram submetidos a patê canino com a presença das L3+ e até o momento somente quatro L1 foram encontradas nas fezes dos cães. Este trabalho demonstra pela primeira vez, a possibilidade de B. glabrata participar como hospedeiro paratênico no ciclo de A. vasorum.Angiostrongylus vasorum is a nematode whose adult forms can be found in the pulmonary arteries and right heart of domestic dogs and wild canids, which are the definitive hosts. In addition to the importance in the small animal clinic, this parasite is discussed in terms of public health due to the possibility of human infection. The heteroxene cycle presents several molluscan species as intermediate hosts, which are infected by ingestion and / or penetration of first stage larvae of the parasite. In the molluscs two seedlings of first instar larvae (L1), L2 and L3 occur, which is infective to the definitive hosts. In addition to these infection routes, there may also be paratenic hosts, which may harbor L3, such as frogs, lizards, mice, rats and birds. Considering that several issues inherent to the cycle of this parasite still remain obscure, the present study aimed to evaluate if Biomphalaria glabrata can be paratenic host of Angiostrongylus vasorum, identifying the possible routes of penetration of these larvae, the migratory route, besides describing the possible changes at different times of infection. Third instar larvae were recovered from B. glabrata molluscs 30 days after infection with 1.000 L1 of A. vasorum using the modified Baermann method. The recovered L3 were used for infection of mollusks of the species B. glabrata, with the inoculum of 100 L3 + (larva of third stage coming from mollusc infected with 100 L3). Third-instar larvae of A. vasorum were recovered alive and active in the mollusc's tissues, thus demonstrating their permissiveness to L3 + infection. The other specimens of infected molluscs were fixed in 10% formaldehyde for histological evaluation, where the presence of larvae in the tissue was observed and they tended to remain in the cephalopodal region. Regarding the viability of L3 +, further studies are still needed, since two mixed breed dogs were submitted to canine pate with the presence of L3 + and so far only four L1 were found in the feces of the dogs. This work demonstrates, for the first time, the possibility of B. glabrata to participate as paratenic host in the A. vasorum cycle.CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorporUniversidade Federal de Minas GeraisPrograma de Pós-Graduação em ParasitologiaUFMGBrasilICB - INSTITUTO DE CIÊNCIAS BIOLOGICAShttp://creativecommons.org/licenses/by-nc-nd/3.0/pt/info:eu-repo/semantics/openAccessDirofilarioseBiomphalariaLarvaAngiostrongylus vasorumBiomphalaria glabrataLarva L3Biomphalaria glabrata (Say, 1818) como hospedeiro paratênico de Angiostrongylus vasorum (Baillet,1866) Kamensky, 1905info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGORIGINALDissertação MESTRADO ISABELA RESENDE ÁVILA.pdfDissertação MESTRADO ISABELA RESENDE ÁVILA.pdfapplication/pdf2416357https://repositorio.ufmg.br/bitstream/1843/35402/1/Disserta%c3%a7%c3%a3o%20MESTRADO%20ISABELA%20RESENDE%20%c3%81VILA.pdfbef2d46f25017b86179adc7d05cf87e8MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufmg.br/bitstream/1843/35402/2/license_rdfcfd6801dba008cb6adbd9838b81582abMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82119https://repositorio.ufmg.br/bitstream/1843/35402/3/license.txt34badce4be7e31e3adb4575ae96af679MD531843/354022021-03-25 12:25:17.292oai:repositorio.ufmg.br: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Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oaiopendoar:2021-03-25T15:25:17Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
|