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2023-12-11T22:56:23Z2023-12-11T22:56:23Z2020108https://doi.org/10.1016/j.jbiomech.2020.1098801873-2380http://hdl.handle.net/1843/61904https://orcid.org/0000-0001-9623-7835https://orcid.org/0000-0001-6078-8408https://orcid.org/0000-0001-7643-6589https://orcid.org/0000-0002-7543-9603https://orcid.org/0000-0002-8681-6451https://orcid.org/0000-0002-1695-0612https://orcid.org/0000-0002-9400-8833https://orcid.org/0000-0002-5071-8382https://orcid.org/0000-0003-0072-3738The aim of this study was to analyze the effect of rapid maxillary expansion (RME) on hard tissues. Opening loops bonded to the first and second maxillary molars on both sides were used to apply distracting forces of 0.28 N, 0.42 N and 0.56 N at the midpalatal suture for 7 and 14 days. Microcomputed tomography (MicroCT), histomorphometry and quantitative polymerase chain reaction (qPCR) analysis were performed to evaluate RME effectiveness, midpalatal suture remodeling, cell counting of osteoblasts, osteoclasts and chondrocytes and the expression of bone remodeling markers, respectively. All forces at the two different time points resulted in similar RME and enhanced of bone remodeling. Accordingly, increased number of osteoblasts and reduced chondrocytes counting and no difference in osteoclasts were seen after all RME protocols. RME yielded increased expression of bone remodeling markers as osteocalcin (Ocn), dentin matrix acidic phosphoprotein-1 (Dmp1), runt-related transcription factor 2 (Runx2), collagen type I Alpha 1 (Col1a1), alkaline phosphatase (ALP), receptor activator of nuclear factor kappa B (RANK), receptor activator of nuclear factor kappa B ligand (Rankl), osteoprotegerin (Opg), cathepsin K (Ctsk), matrix metalloproteinases 9 and 13 (Mmp9 and 13), transforming growth fator beta 1, 2 and 3 (Tgfb 1, Tgfb 2 and Tgfb3), bone morphogenetic protein 2 (Bmp-2), sclerostin (Sost), beta-catenin-like protein 1 (Ctnnbl) and Wnt signaling pathways 3, 3a and 5a (Wnt 3, Wnt 3a and Wnt 5a). These findings characterize the cellular changes and potential molecular pathways involved in RME, proving the reliability of this protocol as a model for mechanical-induced bone remodeling.O objetivo deste estudo foi analisar o efeito da expansão rápida da maxila (ERM) nos tecidos duros. Alças de abertura coladas nos primeiros e segundos molares superiores em ambos os lados foram utilizadas para aplicar forças de distração de 0,28 N, 0,42 N e 0,56 N na sutura palatina média por 7 e 14 dias. Tomografia microcomputadorizada (MicroCT), histomorfometria e análise quantitativa da reação em cadeia da polimerase (qPCR) foram realizadas para avaliar a eficácia da ERM, remodelação da sutura palatina média, contagem de células de osteoblastos, osteoclastos e condrócitos e expressão de marcadores de remodelação óssea, respectivamente. Todas as forças nos dois momentos diferentes resultaram em ERM semelhante e aumento da remodelação óssea. Consequentemente, aumento do número de osteoblastos e redução da contagem de condrócitos e nenhuma diferença nos osteoclastos foram observados após todos os protocolos de ERM. O RME produziu expressão aumentada de marcadores de remodelação óssea como osteocalcina (Ocn), fosfoproteína-1 ácida da matriz dentinária (Dmp1), fator de transcrição 2 relacionado ao runt (Runx2), colágeno tipo I Alfa 1 (Col1a1), fosfatase alcalina (ALP), receptor ativador do fator nuclear kappa B (RANK), ativador do receptor do ligante do fator nuclear kappa B (Rankl), osteoprotegerina (Opg), catepsina K (Ctsk), metaloproteinases de matriz 9 e 13 (Mmp9 e 13), fator transformador de crescimento beta 1, 2 e 3 (Tgfb 1, Tgfb 2 e Tgfb3), proteína morfogenética óssea 2 (Bmp-2), esclerostina (Sost), proteína semelhante à beta-catenina 1 (Ctnnbl) e vias de sinalização Wnt 3, 3a e 5a (Wnt 3 , Wnt 3a e Wnt 5a). Esses achados caracterizam as alterações celulares e potenciais vias moleculares envolvidas na ERM, comprovando a confiabilidade deste protocolo como modelo para remodelação óssea induzida mecanicamente.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaengUniversidade Federal de Minas GeraisUFMGBrasilENG - DEPARTAMENTO DE ENGENHARIA ESTRUTURASFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORAICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIAJournal of biomechanicsExpansão maxilarOrtodontiaRemodelação ósseaModelos teóricosBiomarcadoresMaxillary suture expansionOrthodonticsBone remodelingExperimental modelBiomarkersMolecular analysisMaxillary suture expansion: a mouse model to explore the molecular effects of mechanically-induced bone remodelingExpansão da sutura maxilar: um modelo em camundongo para explorar os efeitos moleculares da remodelação óssea induzida mecanicamenteinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S0021929020303031José Alejandro GuerreroTarcília Aparecida da SilvaEstevam Barbosa de Las CasasSoraia MacariRaquel Souto SilvaIzabella Lucas de Abreu LimaBianca Cristina Duffles RodriguesBreno Rocha BarrioniFlávio Almeida AmaralAndré Petenuci TabanezGustavo Pompermaier GarletDiego Alexander Garzon Alvaradoapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/61904/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALMaxillary suture expansion_ a mouse model to explore the molecular effects of mechanically-induced bone remodeling.pdfMaxillary suture expansion_ a mouse model to explore the molecular effects of mechanically-induced bone remodeling.pdfapplication/pdf4438223https://repositorio.ufmg.br/bitstream/1843/61904/2/Maxillary%20suture%20expansion_%20a%20mouse%20model%20to%20explore%20the%20molecular%20effects%20of%20mechanically-induced%20bone%20remodeling.pdf3fa15a075b62b50176d966bda9abdc83MD521843/619042023-12-11 19:56:24.008oai:repositorio.ufmg.br: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Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-12-11T22:56:24Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
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