DNA damage-related proteins in smokers and non-smokers with oral cancer
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.1590/1807-3107bor-2022.vol36.0027 http://hdl.handle.net/1843/60974 https://orcid.org/0000-0001-8794-5725 |
Resumo: | CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
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2023-11-14T20:34:47Z2023-11-14T20:34:47Z2022-02-093619https://doi.org/10.1590/1807-3107bor-2022.vol36.002718073107http://hdl.handle.net/1843/60974https://orcid.org/0000-0001-8794-5725CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorTobacco smoking involves a high risk of human malignancies, including oral cancer, because it contains multiple carcinogens that cause genetic instability. Thus, a worse prognosis would be expected for cancer patients who are smokers. The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). Associations between immunoexpression of proteins and clinicopathological data and histopathological grading were also analyzed. A total of 35 individuals (18 non-smokers and 17 smokers) with OSCC of the tongue and/or floor of the mouth were included. Immunohistochemistry for H2AX was conducted for the identification of double-strand breaks, CHK2, and P53 to evaluate the expression of this protein in cell cycle regulation. The sample consisted of 22 males and 13 females, with a mean age of 63.9±11.8 years. OSCC of non-smokers were well-differentiated tumors in 50% of the cases, and those of smokers were equally distributed into moderately differentiated and poorly differentiated tumors (35.3% each). Overall, 31 (88.6%) cases were CHK2-positive, 27 (77.1%) were H2AX-positive, and 23 (65.7%) were P53-positive, with no difference between smokers and non-smokers (p > 0.05). No association was found between proteins and clinicopathologic data (p > 0.05). Similarities in CHK2, H2AX, and P53 immunohistochemical staining patterns were observed between smokers and non-smokers, and immunoexpression was not associated with clinicopathological parameters. However, the findings indicated consistent expression of these proteins in OSCC.engUniversidade Federal de Minas GeraisUFMGBrasilFAO - DEPARTAMENTO DE ODONTOLOGIA SOCIAL E PREVENTIVAICB - DEPARTAMENTO DE PATOLOGIABrazilian Oral ResearchNeoplasias bucaisDano ao DNATabagismoDNA damageCheckpoint kinase 2Tumor suppressor protein p53Mouth neoplasmsDNA damage-related proteins in smokers and non-smokers with oral cancerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/bor/a/Hj5J5tHLyL4zfTMJyHzWGvD/?lang=enLauren Frenzel SchuchJosé Alcides Almeida de ArrudaMauro Henrique Nogueira Guimarães de AbreuKarolina Skarlet Silva VianaPatrícia Carlos CaldeiraMaria Cássia Ferreira AguiarVanessa Fátima Bernardesapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/60974/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALDNA damage-related proteins in smokers and non-smokers with oral cancer.pdfDNA damage-related proteins in smokers and non-smokers with oral cancer.pdfapplication/pdf594986https://repositorio.ufmg.br/bitstream/1843/60974/2/DNA%20damage-related%20proteins%20in%20smokers%20and%20non-smokers%20with%20oral%20cancer.pdff9a9bc9e1af038edb6871eea73de4977MD521843/609742023-11-14 17:34:47.823oai:repositorio.ufmg.br:1843/60974Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-11-14T20:34:47Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
| title |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
| spellingShingle |
DNA damage-related proteins in smokers and non-smokers with oral cancer Lauren Frenzel Schuch DNA damage Checkpoint kinase 2 Tumor suppressor protein p53 Mouth neoplasms Neoplasias bucais Dano ao DNA Tabagismo |
| title_short |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
| title_full |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
| title_fullStr |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
| title_full_unstemmed |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
| title_sort |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
| author |
Lauren Frenzel Schuch |
| author_facet |
Lauren Frenzel Schuch José Alcides Almeida de Arruda Mauro Henrique Nogueira Guimarães de Abreu Karolina Skarlet Silva Viana Patrícia Carlos Caldeira Maria Cássia Ferreira Aguiar Vanessa Fátima Bernardes |
| author_role |
author |
| author2 |
José Alcides Almeida de Arruda Mauro Henrique Nogueira Guimarães de Abreu Karolina Skarlet Silva Viana Patrícia Carlos Caldeira Maria Cássia Ferreira Aguiar Vanessa Fátima Bernardes |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Lauren Frenzel Schuch José Alcides Almeida de Arruda Mauro Henrique Nogueira Guimarães de Abreu Karolina Skarlet Silva Viana Patrícia Carlos Caldeira Maria Cássia Ferreira Aguiar Vanessa Fátima Bernardes |
| dc.subject.por.fl_str_mv |
DNA damage Checkpoint kinase 2 Tumor suppressor protein p53 Mouth neoplasms |
| topic |
DNA damage Checkpoint kinase 2 Tumor suppressor protein p53 Mouth neoplasms Neoplasias bucais Dano ao DNA Tabagismo |
| dc.subject.other.pt_BR.fl_str_mv |
Neoplasias bucais Dano ao DNA Tabagismo |
| description |
CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
| publishDate |
2022 |
| dc.date.issued.fl_str_mv |
2022-02-09 |
| dc.date.accessioned.fl_str_mv |
2023-11-14T20:34:47Z |
| dc.date.available.fl_str_mv |
2023-11-14T20:34:47Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/1843/60974 |
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https://doi.org/10.1590/1807-3107bor-2022.vol36.0027 |
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18073107 |
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https://orcid.org/0000-0001-8794-5725 |
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https://doi.org/10.1590/1807-3107bor-2022.vol36.0027 http://hdl.handle.net/1843/60974 https://orcid.org/0000-0001-8794-5725 |
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18073107 |
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eng |
| language |
eng |
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Brazilian Oral Research |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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FAO - DEPARTAMENTO DE ODONTOLOGIA SOCIAL E PREVENTIVA ICB - DEPARTAMENTO DE PATOLOGIA |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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