Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/43350 |
Resumo: | Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present as an efcient treatment or prophylactic option. However, this makes it essential that its genetic structure, stability and interaction with the host immune system be thoroughly characterized. The present study analyzed a novel, broad host-range anti-mastitis agent, the T4virus vB_EcoM-UFV13 in genomic terms, and its activity against a MPEC strain in an experimental E. coli-induced mastitis mouse model. 4,975 Single Nucleotide Polymorphisms (SNPs) were assigned between vB_EcoM-UFV13 and E. coli phage T4 genomes with high impact on coding sequences (CDS) (37.60%) for virion proteins. Phylogenetic trees and genome analysis supported a recent infection mix between vB_EcoM-UFV13 and Shigella phage Shf2. After a viral stability evaluation (e.g pH and temperature), intramammary administration (MOI 10) resulted in a 10-fold reduction in bacterial load. Furthermore, pro-infammatory cytokines, such as IL-6 and TNF-α, were observed after viral treatment. This work brings the whole characterization and immune response to vB_EcoM-UFV13, a biocontrol candidate for bovine mastitis. |
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2022-07-15T20:04:51Z2022-07-15T20:04:51Z201810.1038/s41598-018-24896-w2045-2322http://hdl.handle.net/1843/43350Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present as an efcient treatment or prophylactic option. However, this makes it essential that its genetic structure, stability and interaction with the host immune system be thoroughly characterized. The present study analyzed a novel, broad host-range anti-mastitis agent, the T4virus vB_EcoM-UFV13 in genomic terms, and its activity against a MPEC strain in an experimental E. coli-induced mastitis mouse model. 4,975 Single Nucleotide Polymorphisms (SNPs) were assigned between vB_EcoM-UFV13 and E. coli phage T4 genomes with high impact on coding sequences (CDS) (37.60%) for virion proteins. Phylogenetic trees and genome analysis supported a recent infection mix between vB_EcoM-UFV13 and Shigella phage Shf2. After a viral stability evaluation (e.g pH and temperature), intramammary administration (MOI 10) resulted in a 10-fold reduction in bacterial load. Furthermore, pro-infammatory cytokines, such as IL-6 and TNF-α, were observed after viral treatment. This work brings the whole characterization and immune response to vB_EcoM-UFV13, a biocontrol candidate for bovine mastitis.A mastite bovina continua sendo a principal causa de perdas econômicas para os produtores de leite. Patogênico mamário A Escherichia coli (MPEC) está relacionada a uma mastite aguda e seu tratamento ainda se baseia no uso de antibióticos. Na era da resistência antimicrobiana (RAM), os vírus bacterianos (bacteriófagos) apresentam-se como tratamento eficaz ou opção profilática. No entanto, isso torna essencial que sua estrutura genética, estabilidade e interação com o sistema imunológico do hospedeiro sejam completamente caracterizadas. O presente estudo analisaram um novo agente antimastite de ampla gama de hospedeiros, o T4virus vB_EcoM-UFV13 em termos genômicos, e sua atividade contra uma cepa MPEC em um modelo experimental de camundongo com mastite induzida por E. coli. 4.975 Polimorfismos de nucleotídeo único (SNPs) foram atribuídos entre vB_EcoM-UFV13 e fago T4 de E. coli genomas com alto impacto nas seqüências de codificação (CDS) (37,60%) para proteínas virion. Árvores filogenéticas e análise do genoma apoiou uma mistura de infecção recente entre vB_EcoM-UFV13 e fago Shigella Shf2. Após uma avaliação da estabilidade viral (por exemplo, pH e temperatura), administração intramamária (MOI 10) resultou em uma redução de 10 vezes na carga bacteriana. Além disso, citocinas pró-inflamatórias, como IL-6 e TNF-α, foram observados após o tratamento viral. Este trabalho traz toda a caracterização e imunidade resposta ao vB_EcoM-UFV13, um candidato a biocontrole para mastite bovina.engUniversidade Federal de Minas GeraisUFMGBrasilCOLTEC - COLEGIO TECNICOScientific reportsMastite BovinaAgente AntimastiteBiocontroleGenomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cowsAnálise genômica e resposta imune em modelo de mastite murina de vB_EcoM-UFV13, um potencial agente de biocontrole para uso em vacas leiteirasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.nature.com/articles/s41598-018-24896-wViníciusda Silva DuarteFranciele MartinsJosicelli Souza CrispimAndré da Silva XavierCamila Geovana FerroPedro VidigalCynthia Canêdo da SilvaSérgio Oliveira de PaulaRoberto Sousa DiasAndrew KropinskiStefano CampanaroLaura TreuCarolina SiqueiraMarcella Silva VieiraIsabela da Silva PaesGabriele Rocha Santanaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGORIGINAL2018_Genomic analysis and immune response in a murine mastitis model of vb_ecom-ufv13, a potential biocontrol agent for use in dairy cows.pdf2018_Genomic analysis and immune response in a murine mastitis model of vb_ecom-ufv13, a potential biocontrol agent for use in dairy cows.pdfapplication/pdf3658764https://repositorio.ufmg.br/bitstream/1843/43350/2/2018_Genomic%20analysis%20and%20immune%20response%20in%20a%20murine%20mastitis%20model%20of%20vb_ecom-ufv13%2c%20a%20potential%20biocontrol%20agent%20for%20use%20in%20dairy%20cows.pdfed4b2eccf6f56d8bb85f8d25d51d4583MD52LICENSELicense.txtLicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
dc.title.alternative.pt_BR.fl_str_mv |
Análise genômica e resposta imune em modelo de mastite murina de vB_EcoM-UFV13, um potencial agente de biocontrole para uso em vacas leiteiras |
title |
Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
spellingShingle |
Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows Viníciusda Silva Duarte Mastite Bovina Agente Antimastite Biocontrole |
title_short |
Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
title_full |
Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
title_fullStr |
Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
title_full_unstemmed |
Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
title_sort |
Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
author |
Viníciusda Silva Duarte |
author_facet |
Viníciusda Silva Duarte Franciele Martins Josicelli Souza Crispim André da Silva Xavier Camila Geovana Ferro Pedro Vidigal Cynthia Canêdo da Silva Sérgio Oliveira de Paula Roberto Sousa Dias Andrew Kropinski Stefano Campanaro Laura Treu Carolina Siqueira Marcella Silva Vieira Isabela da Silva Paes Gabriele Rocha Santana |
author_role |
author |
author2 |
Franciele Martins Josicelli Souza Crispim André da Silva Xavier Camila Geovana Ferro Pedro Vidigal Cynthia Canêdo da Silva Sérgio Oliveira de Paula Roberto Sousa Dias Andrew Kropinski Stefano Campanaro Laura Treu Carolina Siqueira Marcella Silva Vieira Isabela da Silva Paes Gabriele Rocha Santana |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Viníciusda Silva Duarte Franciele Martins Josicelli Souza Crispim André da Silva Xavier Camila Geovana Ferro Pedro Vidigal Cynthia Canêdo da Silva Sérgio Oliveira de Paula Roberto Sousa Dias Andrew Kropinski Stefano Campanaro Laura Treu Carolina Siqueira Marcella Silva Vieira Isabela da Silva Paes Gabriele Rocha Santana |
dc.subject.other.pt_BR.fl_str_mv |
Mastite Bovina Agente Antimastite Biocontrole |
topic |
Mastite Bovina Agente Antimastite Biocontrole |
description |
Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present as an efcient treatment or prophylactic option. However, this makes it essential that its genetic structure, stability and interaction with the host immune system be thoroughly characterized. The present study analyzed a novel, broad host-range anti-mastitis agent, the T4virus vB_EcoM-UFV13 in genomic terms, and its activity against a MPEC strain in an experimental E. coli-induced mastitis mouse model. 4,975 Single Nucleotide Polymorphisms (SNPs) were assigned between vB_EcoM-UFV13 and E. coli phage T4 genomes with high impact on coding sequences (CDS) (37.60%) for virion proteins. Phylogenetic trees and genome analysis supported a recent infection mix between vB_EcoM-UFV13 and Shigella phage Shf2. After a viral stability evaluation (e.g pH and temperature), intramammary administration (MOI 10) resulted in a 10-fold reduction in bacterial load. Furthermore, pro-infammatory cytokines, such as IL-6 and TNF-α, were observed after viral treatment. This work brings the whole characterization and immune response to vB_EcoM-UFV13, a biocontrol candidate for bovine mastitis. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2022-07-15T20:04:51Z |
dc.date.available.fl_str_mv |
2022-07-15T20:04:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/43350 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1038/s41598-018-24896-w |
dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
identifier_str_mv |
10.1038/s41598-018-24896-w 2045-2322 |
url |
http://hdl.handle.net/1843/43350 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Scientific reports |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
COLTEC - COLEGIO TECNICO |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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UFMG |
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