MutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical study
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.4317%2Fmedoral.25138 http://hdl.handle.net/1843/61014 https://orcid.org/0000-0002-6657-4547 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2023-11-16T21:18:37Z2023-11-16T21:18:37Z2022-02-20272e164e173https://doi.org/10.4317%2Fmedoral.251381698-6946http://hdl.handle.net/1843/61014https://orcid.org/0000-0002-6657-4547CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPESP - Fundação de Amparo à Pesquisa do Estado de São PauloBackground Appropriate DNA replication is vital to maintain cell integrity at the genomic level. Malfunction on DNA repair mechanisms can have implications related to tumor behavior. Our aim was to evaluate the expression of key complexes of the DNA mismatch-repair system MutSα (hMSH2-hMSH6) and MutSβ (hMSH2-hMSH3) in a panel comprising the most common benign and malignant salivary gland tumors (SGT), and to determine their association with disease-free survival. Material and Methods Ten cases of normal salivary gland (NSG) and 92 of SGT (54 benign and 38 malignant) were retrieved. Immunohistochemistry was performed for hMSH2, hMSH3, hMSH6. Scanned slides were digitally analyzed based on the percentage of positive cells with nuclear staining. Cases were further classified in MutSαhigh and MutSβhigh based on hMSH2-hMSH6 and hMSH3-hMSH6 expression, respectively. Results hMSH3 expression was lower in malignant SGT compared to NSG and benign cases. Adenoid cystic carcinoma (ACC) cases with perineural invasion presented a lower percentage of hMSH3 positive cells. hMSH6 was downregulated in both benign and malignant SGT compared to NSG. Malignant SGT cases with MutSαhigh expression had lower disease-free survival compared to MutSαlow cases. A 10.26-fold increased risk of presenting local recurrence was observed. Conclusions Our findings suggest that a lack of hMSH3 protein function is associated with a more aggressive phenotype (malignancy and perineural invasion) and that MutSα overexpression predicts a poor clinical outcome in malignant SGT.engUniversidade Federal de Minas GeraisUFMGBrasilFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORAFAO - DEPARTAMENTO DE ODONTOPEDIATRIA E ORTODONTIAMed Oral Patol Oral Cir BucalTumoresBiomarcadoresGlândulas SalivaresReparo do DNANeoplasiasSalivary Gland NeoplasmsSalivary gland cancerDNA-repairBiomarkersPrognosisMutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8898574/Gleyson Kleber do Amaral SilvaVivian Petersen WagnerLaryssa Moura DiasBruno Augusto Linhares Almeida MarizFelipe Paiva FonsecaAna Lúcia Carrinho Ayroza RangelVirgilio Gonzales ZanellaRogério Moraes CastilhoManoela Domingues MartinsPablo Agustin Vargasapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/61014/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALMutSα expression predicts a lower disease-free survival in malignant salivary gland tumors_ an immunohistochemical study.pdfMutSα expression predicts a lower disease-free survival in malignant salivary gland tumors_ an immunohistochemical study.pdfapplication/pdf7923453https://repositorio.ufmg.br/bitstream/1843/61014/2/MutS%ce%b1%20expression%20predicts%20a%20lower%20disease-free%20survival%20in%20malignant%20salivary%20gland%20tumors_%20an%20immunohistochemical%20study.pdf05b794a62c1a0a341536b72311088a74MD521843/610142023-11-16 18:18:37.599oai:repositorio.ufmg.br:1843/61014TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-11-16T21:18:37Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
MutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical study |
| title |
MutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical study |
| spellingShingle |
MutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical study Gleyson Kleber do Amaral Silva Salivary Gland Neoplasms Salivary gland cancer DNA-repair Biomarkers Prognosis Tumores Biomarcadores Glândulas Salivares Reparo do DNA Neoplasias |
| title_short |
MutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical study |
| title_full |
MutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical study |
| title_fullStr |
MutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical study |
| title_full_unstemmed |
MutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical study |
| title_sort |
MutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical study |
| author |
Gleyson Kleber do Amaral Silva |
| author_facet |
Gleyson Kleber do Amaral Silva Vivian Petersen Wagner Laryssa Moura Dias Bruno Augusto Linhares Almeida Mariz Felipe Paiva Fonseca Ana Lúcia Carrinho Ayroza Rangel Virgilio Gonzales Zanella Rogério Moraes Castilho Manoela Domingues Martins Pablo Agustin Vargas |
| author_role |
author |
| author2 |
Vivian Petersen Wagner Laryssa Moura Dias Bruno Augusto Linhares Almeida Mariz Felipe Paiva Fonseca Ana Lúcia Carrinho Ayroza Rangel Virgilio Gonzales Zanella Rogério Moraes Castilho Manoela Domingues Martins Pablo Agustin Vargas |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Gleyson Kleber do Amaral Silva Vivian Petersen Wagner Laryssa Moura Dias Bruno Augusto Linhares Almeida Mariz Felipe Paiva Fonseca Ana Lúcia Carrinho Ayroza Rangel Virgilio Gonzales Zanella Rogério Moraes Castilho Manoela Domingues Martins Pablo Agustin Vargas |
| dc.subject.por.fl_str_mv |
Salivary Gland Neoplasms Salivary gland cancer DNA-repair Biomarkers Prognosis |
| topic |
Salivary Gland Neoplasms Salivary gland cancer DNA-repair Biomarkers Prognosis Tumores Biomarcadores Glândulas Salivares Reparo do DNA Neoplasias |
| dc.subject.other.pt_BR.fl_str_mv |
Tumores Biomarcadores Glândulas Salivares Reparo do DNA Neoplasias |
| description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| publishDate |
2022 |
| dc.date.issued.fl_str_mv |
2022-02-20 |
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2023-11-16T21:18:37Z |
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2023-11-16T21:18:37Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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http://hdl.handle.net/1843/61014 |
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https://doi.org/10.4317%2Fmedoral.25138 |
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1698-6946 |
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https://orcid.org/0000-0002-6657-4547 |
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https://doi.org/10.4317%2Fmedoral.25138 http://hdl.handle.net/1843/61014 https://orcid.org/0000-0002-6657-4547 |
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1698-6946 |
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eng |
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eng |
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Med Oral Patol Oral Cir Bucal |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA FAO - DEPARTAMENTO DE ODONTOPEDIATRIA E ORTODONTIA |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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