Avaliação da imunidade cruzada entre Leptospiras hardjo e wolffi

Detalhes bibliográficos
Autor(a) principal: Maria Carmen de Rezende Costa
Data de Publicação: 1996
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFMG
Texto Completo: http://hdl.handle.net/1843/BUOS-8QGLZ4
Resumo: The cross-immunity between Leptospira hardjo and L. wolffi was experimentally evaluated in four groups of adult healthy golden hamsters. Each animal in groups I and ll were vaccinated. respectively with hardjo and wolffi. 18 days alter the vaccination, All the animals, except those in the negative control group, received a challenge dose of a virulent sample of hardjo. This sample was 100 X (times) greater than the infectious dose (ID 50) of hardjo previously activated through serial passages in hamsters. The achieved protection was evaluated by the direct finding of leptospiras (slides silver stained: Warthin-Starry and cultures of macerated organs in Fletcher and in modificated EMJH media). Clinical signs and histological lesions in liver, kidney and lungs HE stained slides were also analysed to detect the presence of leptospiras. The sera titres ofthe hamsters was tested by fast microaglutination in plates. The results strongly suggested that the vaccination of the animals either with hardjo or wolffi developed immunity against the harzyo strain.
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spelling Avaliação da imunidade cruzada entre Leptospiras hardjo e wolffivacinahamsterLeptospira hardjoLeptospirose Vacina Modelo animalLeptospira ControleThe cross-immunity between Leptospira hardjo and L. wolffi was experimentally evaluated in four groups of adult healthy golden hamsters. Each animal in groups I and ll were vaccinated. respectively with hardjo and wolffi. 18 days alter the vaccination, All the animals, except those in the negative control group, received a challenge dose of a virulent sample of hardjo. This sample was 100 X (times) greater than the infectious dose (ID 50) of hardjo previously activated through serial passages in hamsters. The achieved protection was evaluated by the direct finding of leptospiras (slides silver stained: Warthin-Starry and cultures of macerated organs in Fletcher and in modificated EMJH media). Clinical signs and histological lesions in liver, kidney and lungs HE stained slides were also analysed to detect the presence of leptospiras. The sera titres ofthe hamsters was tested by fast microaglutination in plates. The results strongly suggested that the vaccination of the animals either with hardjo or wolffi developed immunity against the harzyo strain.Neste trabalho avaliou-se a intimidade cruzada entre as leptospiras hardjo e wolffi, em quatro grupos de hamsters adultos e sadios. O primeiro e o segundo grupos foram constituídos por hamsters vacinados, respectivamente, com hardjo e wolffi. Os outros grupos foram os controles um positive e outro negativo. Com exceção do grupo controle negativo, todos os animais receberam um desafio 18 dias após a vacinação. Esse inóculo-desafio constituiu-se de 100 vezes a dose infectante 50 (DI 50) de hardjo previamente ativada através de uma série de passagens em hamsters. A proteção conferida pelas vacinas foi avaliada através de pesquisa direta de leptospiras (pelos métodos de cultura de macerado de órgãos em meios de Fletcher e EMJH modificado e histopatologia com coloração pela prata: Warthin-Starry) e pesquisa indireta de leptospiras (avaliação de sinais clínicos e estudo histopatológico de fígado, rim e pulmão pela coloração hematoxilina-eosina dos cortes histológicos). O título sorológico dos hamsters foi avaliado pela Microaglutiuação Rápida em Placas. Os resultados revelaram que a vacinação dos hamsters tanto com hardjo quanto com wolffi conferiram intimidade frente ao desafio com hardjo.Universidade Federal de Minas GeraisUFMGElvio Carlos MoreiraRomulo Cerqueira LeiteNelson Rodrigo da Silva MartinsPedro Moacyr Pinto Coelho MotaFrancisco BaptistaMaria Carmen de Rezende Costa2019-08-13T12:25:32Z2019-08-13T12:25:32Z1996-02-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/1843/BUOS-8QGLZ4info:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2019-11-15T01:58:23Zoai:repositorio.ufmg.br:1843/BUOS-8QGLZ4Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2019-11-15T01:58:23Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Avaliação da imunidade cruzada entre Leptospiras hardjo e wolffi
title Avaliação da imunidade cruzada entre Leptospiras hardjo e wolffi
spellingShingle Avaliação da imunidade cruzada entre Leptospiras hardjo e wolffi
Maria Carmen de Rezende Costa
vacina
hamster
Leptospira hardjo
Leptospirose Vacina Modelo animal
Leptospira Controle
title_short Avaliação da imunidade cruzada entre Leptospiras hardjo e wolffi
title_full Avaliação da imunidade cruzada entre Leptospiras hardjo e wolffi
title_fullStr Avaliação da imunidade cruzada entre Leptospiras hardjo e wolffi
title_full_unstemmed Avaliação da imunidade cruzada entre Leptospiras hardjo e wolffi
title_sort Avaliação da imunidade cruzada entre Leptospiras hardjo e wolffi
author Maria Carmen de Rezende Costa
author_facet Maria Carmen de Rezende Costa
author_role author
dc.contributor.none.fl_str_mv Elvio Carlos Moreira
Romulo Cerqueira Leite
Nelson Rodrigo da Silva Martins
Pedro Moacyr Pinto Coelho Mota
Francisco Baptista
dc.contributor.author.fl_str_mv Maria Carmen de Rezende Costa
dc.subject.por.fl_str_mv vacina
hamster
Leptospira hardjo
Leptospirose Vacina Modelo animal
Leptospira Controle
topic vacina
hamster
Leptospira hardjo
Leptospirose Vacina Modelo animal
Leptospira Controle
description The cross-immunity between Leptospira hardjo and L. wolffi was experimentally evaluated in four groups of adult healthy golden hamsters. Each animal in groups I and ll were vaccinated. respectively with hardjo and wolffi. 18 days alter the vaccination, All the animals, except those in the negative control group, received a challenge dose of a virulent sample of hardjo. This sample was 100 X (times) greater than the infectious dose (ID 50) of hardjo previously activated through serial passages in hamsters. The achieved protection was evaluated by the direct finding of leptospiras (slides silver stained: Warthin-Starry and cultures of macerated organs in Fletcher and in modificated EMJH media). Clinical signs and histological lesions in liver, kidney and lungs HE stained slides were also analysed to detect the presence of leptospiras. The sera titres ofthe hamsters was tested by fast microaglutination in plates. The results strongly suggested that the vaccination of the animals either with hardjo or wolffi developed immunity against the harzyo strain.
publishDate 1996
dc.date.none.fl_str_mv 1996-02-29
2019-08-13T12:25:32Z
2019-08-13T12:25:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/BUOS-8QGLZ4
url http://hdl.handle.net/1843/BUOS-8QGLZ4
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
UFMG
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
UFMG
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
_version_ 1823248072172371968

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