Influence of genetic regulatory effects modified by environmental immune activation on periapical disease.

• Autor(a) principal: Nely Cristina Medeiros Caires
• Data de Publicação: 2019
• Outros Autores: Marcela Carvalho Espaladori, Warley Luciano Fonseca Tavares, Luciana Carla Neves de Brito, Leda Quercia Vieira, Antônio Paulino Ribeiro Sobrinho
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.1590/1807-3107bor-2019.vol33.0109; http://hdl.handle.net/1843/56239; https://orcid.org/0000-0003-3094-4064; https://orcid.org/0000-0003-1868-2006; https://orcid.org/0000-0003-2077-927X; https://orcid.org/0000-0001-5481-9618; https://orcid.org/0000-0002-3598-7592
• topic: Cytokines; Root canal therapy; Population groups; Indigenous population; Citocinas; Tratamento do canal radicular; Grupos populacionais; Povos Indígenas

The objective of this study was to compare the periradicular responses in endodontic infections among members of two populations: an urban Brazilian population and a non-mixed indigenous population. Samples were collected immediately and 7 days after the cleaning and shaping procedures (after reducing the intracanal microbial load) in an attempt to characterize the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-9, interferon (IFN)-γ, IL-17, IL-10, and the chemokines CXCR4, CCL2/monocyte chemotactic protein (MCP)-1, and CCR6. The endogenous cytokine and chemokine expression levels were analyzed using real-time PCR. Only the urban population showed a significant increase in TNF-α, CCL2/MCP-1, CXCR4, and CCR6 expression following the cleaning and shaping of the root canal system. The IFN-γ levels were increased at the 2nd collection (p < 0.05) in the indigenous population. In turn, a significant increase in IL-10 and IL-17 expression (p < 0.05) was observed after the cleaning and shaping procedures (2nd collection) in both populations. No significant differences in the IL-1β, IL-9, and CCL4 expression levels were observed between the 1st and 2nd collections in both populations. The results demonstrate a cytokine and chemokine expression profile that is specific to each analyzed population. However, immune modulation mediated by IL-10 began on the 7th day after the beginning of the endodontic treatment in both populations.