Salivary molecules of bone remodeling and tissue repair after head and neck radiotherapy

• Autor(a) principal: Eduardo Morato de Oliveira
• Data de Publicação: 2021
• Outros Autores: Rafael Soares Guieiro, Bárbara Lima Cruz, Maria Cássia Ferreira de Aguiar, Janine Mayra da Silva, Tarcília Aparecida da Silva, Patrícia Carlos Caldeira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.1590/1807-3107bor-2021.vol35.0079; http://hdl.handle.net/1843/74279
• topic: Saliva; Saliva; Radiotherapy; Bone remodeling; Bone remodeling; Mouth neoplasms; Head and neck neoplasms; Biomarkers; Receptor activator of nuclear factor-kappa B; RANK ligand; Osteoprotegerin; Osteoprotegerin; Matrix Metalloproteinase 9; Tissue inhibitor of Metalloproteinase-2; Endothelial growth factors; Blood; Epidermal growth factor; Homeostasis; Mouth

Head and neck radiotherapy causes quantitative and qualitative changes in saliva. The objective of this case-control study was to evaluate the salivary biomarkers associated with bone remodeling and tissue repair in patients submitted to radiotherapy for head and neck cancer treatment, compared with non-irradiated individuals. Total unstimulated saliva was collected for ELISA assay analysis of receptor activator for nuclear factor κ B (RANK) and its ligand (RANK-L), osteoprotegerin, matrix metalloproteinase-9/ tissue inhibitor of metalloproteinase-2, vascular endothelial growth factor, and epidermal growth factor. Statistics were performed, and revealed that salivary RANK (p = 0.0304), RANK-L (p = 0.0005), matrix metalloproteinase-9/ tissue inhibitor of metalloproteinase-2 (p = 0.0067), vascular endothelial growth factor (p = 0.0060), and epidermal growth factor (p < 0.0001) were reduced in patients, compared with the control group. Osteoprotegerin did not differ between the groups (p = 0.3765). Salivary biomarkers did not differ according to radiotherapy completion time (p > 0.05). In conclusion, the lower output of the salivary molecules – essential for bone remodeling and tissue repair – may disrupt tissue homeostasis and play a role in the pathogenesis of the radiotherapy-induced deleterious effects in the oral cavity.