Evaluation of the gliosis-reducing potential of CTK 01512-2, the recombinant version of Phα1β toxin isolated from the venom of Phoneutria nigriventer, in a Parkinson's disease mouse model

• Autor(a) principal: Matheus Felipe Guimarães Aguiar
• Data de Publicação: 2021
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: http://hdl.handle.net/1843/47264
• topic: 6-OHDA; Parkinson’s disease; Neuroinflammation; Glial scar; CTK 01512-2; Phα1β; Oxidopamine; Parkinson Disease; Gliosis; Doenças Neurodegenerativas; Sistema Nervoso Central

Neuroinflammatory response is important to recover the homeostasis, however in an intense and chronic state it could be harmful to the Central Nervous System. Efforts to restrict the microglia and astrocytes damage in these cases are being investigated in neurodegenerative diseases, since the excessive reactivity of these cells could contribute to the aggravation of these disorders. Here, we showed striatal microglia and astrocyte response to dopaminergic degeneration in a 6-OHDA Parkinson's disease mouse model, in six different moments within a six months window period. Furthermore, we found that a single intracerebroventricular injection of CTK01512-2, a recombinant version of a Phoneutria nigriventer venom peptide that is capable of reversible inhibition of calcium channel, was able to reduce severe reaction of microglia and astrocytes in scar formation. Thus, as we showed that the CTK 01512-2 anti-inflammatory effect could be applied to severe cases of gliosis with scar formation, we suggest that this potential should be better elucidated.