LNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cells

Detalhes bibliográficos
Autor(a) principal: Leonardo Campos Zanelatto
Data de Publicação: 2016
Outros Autores: Patrícia Benites Gonçalves da Silva, Daniele Sartori, Sandra Lepri, Ângelo de Fátima, Mário Sérgio Mantovani
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1590/1678-4685-GMB-2015-0184
http://hdl.handle.net/1843/64884
https://orcid.org/0000-0002-9091-0766
https://orcid.org/0000-0002-0465-9932
https://orcid.org/0000-0003-2344-5590
https://orcid.org/0000-0003-3479-645X
https://orcid.org/0000-0001-5268-6508
Resumo: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
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spelling 2024-02-28T17:45:36Z2024-02-28T17:45:36Z2016392270278https://doi.org/10.1590/1678-4685-GMB-2015-01841678-4685http://hdl.handle.net/1843/64884https://orcid.org/0000-0002-9091-0766https://orcid.org/0000-0002-0465-9932https://orcid.org/0000-0003-2344-5590https://orcid.org/0000-0003-3479-645Xhttps://orcid.org/0000-0001-5268-6508CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaThe identification of antitumoral substances is the focus of intense biomedical research. Two structural analogues of thalidomide, LNO3 and L3, are two synthetic compounds that might possess such antitumor properties. We evaluated the toxicological effects of these substances, including cytotoxicity, genotoxicity and induction of apoptosis in HTC cells. Additionally, the production of free radicals (nitric oxide and superoxide) was investigated, and the expression of caspases genes 3, 8, and 9 were determined by RT-qPCR. The compounds exhibited cytotoxic effects that resulted in inhibited cell proliferation. LNO3 showed to be more effective and toxic than L3 in all assays. LNO3 stimulated the release of NO and superoxide, which was accompanied by the formation of peroxynitrite. Apoptosis was induced in a dose-dependent manner by both compounds; however, the expression of caspases 3, 8 and 9 was unchanged. These results suggested that L3 and LNO3 possess antiproliferative and pro-apoptotic effects in HTC cells. Additionally, although they exhibited cytotoxicity, L3 and LNO3 might be useful coadjuvants in tumor treatment studies.engUniversidade Federal de Minas GeraisUFMGBrasilICX - DEPARTAMENTO DE QUÍMICAGenetics and Molecular BiologyBioquímicaQuímicaÓxido nítricoReações de radicais livresApoptoseAgentes antineoplásicosNitric oxide donating drugNitric oxideFree radical scavengingApoptosisAntiproliferative activityThalidomide analoguesLNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/gmb/a/VSCtR4XRPdmK7Ydk8xfwS4Q/Leonardo Campos ZanelattoPatrícia Benites Gonçalves da SilvaDaniele SartoriSandra LepriÂngelo de FátimaMário Sérgio Mantovaniapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/64884/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALLNO3 AND L3 Are Associated With Antiproliferative And Pro-Apoptotic Action (1).pdfLNO3 AND L3 Are Associated With Antiproliferative And Pro-Apoptotic Action (1).pdfapplication/pdf588424https://repositorio.ufmg.br/bitstream/1843/64884/2/LNO3%20AND%20L3%20Are%20Associated%20With%20Antiproliferative%20And%20Pro-Apoptotic%20Action%20%281%29.pdf374e7df4b6b6c3868480db5b94a6e353MD521843/648842024-02-28 14:45:37.488oai:repositorio.ufmg.br:1843/64884TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2024-02-28T17:45:37Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv LNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cells
title LNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cells
spellingShingle LNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cells
Leonardo Campos Zanelatto
Nitric oxide donating drug
Nitric oxide
Free radical scavenging
Apoptosis
Antiproliferative activity
Thalidomide analogues
Bioquímica
Química
Óxido nítrico
Reações de radicais livres
Apoptose
Agentes antineoplásicos
title_short LNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cells
title_full LNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cells
title_fullStr LNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cells
title_full_unstemmed LNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cells
title_sort LNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cells
author Leonardo Campos Zanelatto
author_facet Leonardo Campos Zanelatto
Patrícia Benites Gonçalves da Silva
Daniele Sartori
Sandra Lepri
Ângelo de Fátima
Mário Sérgio Mantovani
author_role author
author2 Patrícia Benites Gonçalves da Silva
Daniele Sartori
Sandra Lepri
Ângelo de Fátima
Mário Sérgio Mantovani
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Leonardo Campos Zanelatto
Patrícia Benites Gonçalves da Silva
Daniele Sartori
Sandra Lepri
Ângelo de Fátima
Mário Sérgio Mantovani
dc.subject.por.fl_str_mv Nitric oxide donating drug
Nitric oxide
Free radical scavenging
Apoptosis
Antiproliferative activity
Thalidomide analogues
topic Nitric oxide donating drug
Nitric oxide
Free radical scavenging
Apoptosis
Antiproliferative activity
Thalidomide analogues
Bioquímica
Química
Óxido nítrico
Reações de radicais livres
Apoptose
Agentes antineoplásicos
dc.subject.other.pt_BR.fl_str_mv Bioquímica
Química
Óxido nítrico
Reações de radicais livres
Apoptose
Agentes antineoplásicos
description CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2024-02-28T17:45:36Z
dc.date.available.fl_str_mv 2024-02-28T17:45:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/64884
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1590/1678-4685-GMB-2015-0184
dc.identifier.issn.pt_BR.fl_str_mv 1678-4685
dc.identifier.orcid.pt_BR.fl_str_mv https://orcid.org/0000-0002-9091-0766
https://orcid.org/0000-0002-0465-9932
https://orcid.org/0000-0003-2344-5590
https://orcid.org/0000-0003-3479-645X
https://orcid.org/0000-0001-5268-6508
url https://doi.org/10.1590/1678-4685-GMB-2015-0184
http://hdl.handle.net/1843/64884
https://orcid.org/0000-0002-9091-0766
https://orcid.org/0000-0002-0465-9932
https://orcid.org/0000-0003-2344-5590
https://orcid.org/0000-0003-3479-645X
https://orcid.org/0000-0001-5268-6508
identifier_str_mv 1678-4685
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Genetics and Molecular Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv ICX - DEPARTAMENTO DE QUÍMICA
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
bitstream.url.fl_str_mv https://repositorio.ufmg.br/bitstream/1843/64884/1/License.txt
https://repositorio.ufmg.br/bitstream/1843/64884/2/LNO3%20AND%20L3%20Are%20Associated%20With%20Antiproliferative%20And%20Pro-Apoptotic%20Action%20%281%29.pdf
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