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2023-10-17T00:15:23Z2023-10-17T00:15:23Z20221161102525-3409http://hdl.handle.net/1843/59520O objetivo deste trabalho foi avaliar a atividade antibacteriana e antibiofilme do óleo essencial de cravo-da-índia (Syzygium aromaticum) microencapsulado e não microencapsulado sobre linhagens de Staphylococcus aureus multirresistentes. A atividade antibacteriana foi realizada pelas técnicas de difusão de disco e de microdiluição em caldo para determinação da Concentração Inibitória Mínima (CIM) e da Concentração Bactericida Mínima (CBM) do óleo essencial. Para a atividade antibiofilme, utilizaram-se as linhagens de S. aureus que foram sensíveis a ação do óleo essencial. Cinco isolados foram sensíveis a ação do óleo essencial pelo teste de difusão em disco cuja CIM foi de 10 μL.mL-1 e a CBM de 20 μL.mL-1. Destas cinco, quatro foram capazes de formar biofilme com adesão superior a 107 UFC cm-2. O óleo microencapsulado apresentou melhor efeito antibiofilme com redução de cerca de 2 log UFC.cm-2 nos S. aureus multirresistentes analisadas, e o não microencapsulado reduziu aproximadamente 1,1 log UFC.cm², demonstrado a maior eficiência do óleo com microencapsulação. O uso de óleo essencial de Syzygium aromaticum microencapsulado poderia servir com uma alternativa promissora no controle de bactérias multirresistentes e formadoras de biofilmes em superfícies de aço inoxidável apresentando algumas vantagens em comparação com o utilizado em sua forma original, principalmente no que diz respeito ao transporte, preservação do conteúdo, manipulação e utilização em matrizes alimentares.The aim of this work was to evaluate the antibacterial and antibiofilm activity of microencapsulated and nonmicroencapsulated clove (Syzygium aromaticum) essential oil on multidrug-resistant Staphylococcus aureus strains. Antibacterial activity was performed by disk diffusion and broth microdilution techniques to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the essential oil. For the antibiofilm activity, strains of S. aureus that were sensitive to the action of the essential oil were used. Five isolates were sensitive to the action of the essential oil by the disk diffusion test whose MIC was 10 μL.mL-1 and the CBM was 20 μL.mL-1. Of these five, four were able to form biofilms with adhesion greater than 107 CFU.cm-2. The microencapsulated oil showed a better antibiofilm effect with a reduction of about 2 log CFU.cm-2 in the multiresistant S. aureus analyzed, and the non-microencapsulated oil reduced approximately 1.1 log CFU.cm-2 showing the high efficiency of the oil with microencapsulation. The use of microencapsulated Syzygium aromaticum essential oil could serve as a promising alternative in the control of multiresistant and biofilm forming bacteria on stainless steel surfaces, presenting some advantages compared to the one used in its original form, mainly with regard to transport, content preservation, handling and use in food matrices.porUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASResearch, Society and DevelopmentBiofilmeStaphylococcus aureusMicroencapsulaçãoCravo-da-índiaBiofilmeStaphylococcus aureusMicroencapsulaçãoAtividade antibacteriana e antibiofilme de óleo essencial de Syzygium aromaticum microencapsulado e não microencapsuladoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.33448/rsd-v11i6.29076Daniele Pereira de Souza BorboremaAnna Christina de AlmeidaCintya Neves de SouzaCarolina Magalhães Caires CarvalhoCharles Martins AguilarFernando Eustáquio de Matos JúniorUlisses Alves PereiraEster Dias XavierAlessandra Rejane Ericsson de Oliveira XavierJanaína Teles de Fariainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/59520/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALAtividade antibacteriana e antibiofilme de óleo essencial de Syzygium aromaticum-Janaína Teles de Faria.pdfAtividade antibacteriana e antibiofilme de óleo essencial de Syzygium aromaticum-Janaína Teles de Faria.pdfapplication/pdf136173https://repositorio.ufmg.br/bitstream/1843/59520/2/Atividade%20antibacteriana%20e%20antibiofilme%20de%20%c3%b3leo%20essencial%20de%20Syzygium%20aromaticum-Jana%c3%adna%20Teles%20de%20Faria.pdfa59bb7a5ee8b55be8f621420d0c102a3MD521843/595202023-10-16 21:31:03.063oai:repositorio.ufmg.br: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Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-10-17T00:31:03Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
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