Prognosis biomarkers evaluation in chronic lymphocytic leukemia
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/40286 |
Resumo: | Objective/background: From clinical and biological points of view, chronic lymphocytic leukemia (CLL) is a heterogeneous disease characterized by a progressive accumulation of lymphocytes in the peripheral blood, bone marrow, and lymphoid organs. New prognostic markers in CLL may be useful to clinicians for predicting outcome and in clinical decision-making. The aim of this study was to evaluate the potential prognostic value of the apoptotic/survival-controlling proteins and protein tyrosine kinase ZAP-70 gene expression in CLL patients and control individuals, correlating such findings with patients' clinical data. Methods: Fifty-three patients diagnosed with CLL attending the hematology service of a clinical hospital, and 24 healthy individuals with no history of leukemia (Control group) were enrolled in this study. Analyses of apoptotic/survival-controlling proteins were performed by western blot and ZAP-70 gene expression was evaluated by real-time polymerase chain reaction. Results: Significant differences were observed for the p-p38, Mcl-1 long, and Mcl-1 short proteins when patients were compared with CLL and controls. A positive correlation between the results for Mcl-1 short and Mcl-1 long and lymphocyte count was observed, corroborating the hypothesis of an imbalance between proteins of cell survival pathways/apoptosis in CLL. Conclusion: ZAP-70 gene expression was not detected as a discriminant biomarker in these CLL patients. An imbalance between apoptosis-related proteins was observed in the present study, corroborating the hypothesis of increased survival of lymphocytes in CLL patients. |
id |
UFMG_60f7b8b29dc8341ec12906a9fd955443 |
---|---|
oai_identifier_str |
oai:repositorio.ufmg.br:1843/40286 |
network_acronym_str |
UFMG |
network_name_str |
Repositório Institucional da UFMG |
repository_id_str |
|
spelling |
2022-03-21T20:38:06Z2022-03-21T20:38:06Z2017102576210.1016/j.hemonc.2016.12.00416583876http://hdl.handle.net/1843/40286Objective/background: From clinical and biological points of view, chronic lymphocytic leukemia (CLL) is a heterogeneous disease characterized by a progressive accumulation of lymphocytes in the peripheral blood, bone marrow, and lymphoid organs. New prognostic markers in CLL may be useful to clinicians for predicting outcome and in clinical decision-making. The aim of this study was to evaluate the potential prognostic value of the apoptotic/survival-controlling proteins and protein tyrosine kinase ZAP-70 gene expression in CLL patients and control individuals, correlating such findings with patients' clinical data. Methods: Fifty-three patients diagnosed with CLL attending the hematology service of a clinical hospital, and 24 healthy individuals with no history of leukemia (Control group) were enrolled in this study. Analyses of apoptotic/survival-controlling proteins were performed by western blot and ZAP-70 gene expression was evaluated by real-time polymerase chain reaction. Results: Significant differences were observed for the p-p38, Mcl-1 long, and Mcl-1 short proteins when patients were compared with CLL and controls. A positive correlation between the results for Mcl-1 short and Mcl-1 long and lymphocyte count was observed, corroborating the hypothesis of an imbalance between proteins of cell survival pathways/apoptosis in CLL. Conclusion: ZAP-70 gene expression was not detected as a discriminant biomarker in these CLL patients. An imbalance between apoptosis-related proteins was observed in the present study, corroborating the hypothesis of increased survival of lymphocytes in CLL patients.Objetivo/Contexto: Do ponto de vista clínico e biológico, a leucemia linfocítica crônica (LLC) é uma doença heterogênea caracterizada por um acúmulo progressivo de linfócitos no sangue periférico, medula óssea e órgãos linfoides. Novos marcadores prognósticos na LLC podem ser úteis para os médicos na previsão de resultados e na tomada de decisões clínicas. O objetivo deste estudo foi avaliar o potencial valor prognóstico da expressão do gene ZAP-70 das proteínas apoptóticas/controladoras de sobrevivência e da proteína tirosina quinase ZAP-70 em pacientes com LLC e indivíduos controles, correlacionando tais achados com os dados clínicos dos pacientes. Métodos: Cinquenta e três pacientes com diagnóstico de LLC atendidos no serviço de hematologia de um hospital clínico e 24 indivíduos saudáveis sem histórico de leucemia (grupo controle) foram incluídos neste estudo. Análises de proteínas apoptóticas/controladoras de sobrevivência foram realizadas por western blot e a expressão do gene ZAP-70 foi avaliada por reação em cadeia da polimerase em tempo real. Resultados: Foram observadas diferenças significativas para as proteínas p-p38, Mcl-1 longa e Mcl-1 curta quando os pacientes foram comparados com LLC e controles. Foi observada correlação positiva entre os resultados de Mcl-1 curto e Mcl-1 longo e contagem de linfócitos, corroborando a hipótese de um desequilíbrio entre proteínas das vias de sobrevivência celular/apoptose na LLC. Conclusão: A expressão do gene ZAP-70 não foi detectada como biomarcador discriminante nesses pacientes com LLC. Um desequilíbrio entre as proteínas relacionadas à apoptose foi observado no presente estudo, corroborando a hipótese de aumento da sobrevida dos linfócitos em pacientes com LLC.engUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASHematology/oncology and stem cell therapyApoptose/proteínas de sobrevivênciaLeucemia linfocítica crônicaZAP-70Prognosis biomarkers evaluation in chronic lymphocytic leukemiaAvaliação de biomarcadores de prognóstico na leucemia linfocítica crônicainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S1658387617300067?via%3DihubLorena CaixetagomesFernanda Cristina Gontijo EvangelistaLirlândia Pires de SousaSergio Schusterschitz da Silva AraujoMaria Das Graças CarvalhoAdriano de Paula Sabinoapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/40286/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINAL2017_Prognosis biomarkers evaluation in chronic lymphocytic leukemia.pdf2017_Prognosis biomarkers evaluation in chronic lymphocytic leukemia.pdfapplication/pdf705676https://repositorio.ufmg.br/bitstream/1843/40286/2/2017_Prognosis%20biomarkers%20evaluation%20in%20chronic%20lymphocytic%20leukemia.pdf8d3121978345d26a253ffc9d555b28ecMD521843/402862022-03-21 17:38:06.714oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-03-21T20:38:06Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Prognosis biomarkers evaluation in chronic lymphocytic leukemia |
dc.title.alternative.pt_BR.fl_str_mv |
Avaliação de biomarcadores de prognóstico na leucemia linfocítica crônica |
title |
Prognosis biomarkers evaluation in chronic lymphocytic leukemia |
spellingShingle |
Prognosis biomarkers evaluation in chronic lymphocytic leukemia Lorena Caixetagomes Apoptose/proteínas de sobrevivência Leucemia linfocítica crônica ZAP-70 |
title_short |
Prognosis biomarkers evaluation in chronic lymphocytic leukemia |
title_full |
Prognosis biomarkers evaluation in chronic lymphocytic leukemia |
title_fullStr |
Prognosis biomarkers evaluation in chronic lymphocytic leukemia |
title_full_unstemmed |
Prognosis biomarkers evaluation in chronic lymphocytic leukemia |
title_sort |
Prognosis biomarkers evaluation in chronic lymphocytic leukemia |
author |
Lorena Caixetagomes |
author_facet |
Lorena Caixetagomes Fernanda Cristina Gontijo Evangelista Lirlândia Pires de Sousa Sergio Schusterschitz da Silva Araujo Maria Das Graças Carvalho Adriano de Paula Sabino |
author_role |
author |
author2 |
Fernanda Cristina Gontijo Evangelista Lirlândia Pires de Sousa Sergio Schusterschitz da Silva Araujo Maria Das Graças Carvalho Adriano de Paula Sabino |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lorena Caixetagomes Fernanda Cristina Gontijo Evangelista Lirlândia Pires de Sousa Sergio Schusterschitz da Silva Araujo Maria Das Graças Carvalho Adriano de Paula Sabino |
dc.subject.other.pt_BR.fl_str_mv |
Apoptose/proteínas de sobrevivência Leucemia linfocítica crônica ZAP-70 |
topic |
Apoptose/proteínas de sobrevivência Leucemia linfocítica crônica ZAP-70 |
description |
Objective/background: From clinical and biological points of view, chronic lymphocytic leukemia (CLL) is a heterogeneous disease characterized by a progressive accumulation of lymphocytes in the peripheral blood, bone marrow, and lymphoid organs. New prognostic markers in CLL may be useful to clinicians for predicting outcome and in clinical decision-making. The aim of this study was to evaluate the potential prognostic value of the apoptotic/survival-controlling proteins and protein tyrosine kinase ZAP-70 gene expression in CLL patients and control individuals, correlating such findings with patients' clinical data. Methods: Fifty-three patients diagnosed with CLL attending the hematology service of a clinical hospital, and 24 healthy individuals with no history of leukemia (Control group) were enrolled in this study. Analyses of apoptotic/survival-controlling proteins were performed by western blot and ZAP-70 gene expression was evaluated by real-time polymerase chain reaction. Results: Significant differences were observed for the p-p38, Mcl-1 long, and Mcl-1 short proteins when patients were compared with CLL and controls. A positive correlation between the results for Mcl-1 short and Mcl-1 long and lymphocyte count was observed, corroborating the hypothesis of an imbalance between proteins of cell survival pathways/apoptosis in CLL. Conclusion: ZAP-70 gene expression was not detected as a discriminant biomarker in these CLL patients. An imbalance between apoptosis-related proteins was observed in the present study, corroborating the hypothesis of increased survival of lymphocytes in CLL patients. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2022-03-21T20:38:06Z |
dc.date.available.fl_str_mv |
2022-03-21T20:38:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/40286 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1016/j.hemonc.2016.12.004 |
dc.identifier.issn.pt_BR.fl_str_mv |
16583876 |
identifier_str_mv |
10.1016/j.hemonc.2016.12.004 16583876 |
url |
http://hdl.handle.net/1843/40286 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Hematology/oncology and stem cell therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Repositório Institucional da UFMG |
collection |
Repositório Institucional da UFMG |
bitstream.url.fl_str_mv |
https://repositorio.ufmg.br/bitstream/1843/40286/1/License.txt https://repositorio.ufmg.br/bitstream/1843/40286/2/2017_Prognosis%20biomarkers%20evaluation%20in%20chronic%20lymphocytic%20leukemia.pdf |
bitstream.checksum.fl_str_mv |
fa505098d172de0bc8864fc1287ffe22 8d3121978345d26a253ffc9d555b28ec |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
|
_version_ |
1803589437115858944 |