Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages

Detalhes bibliográficos
Autor(a) principal: Grazielle Ribeiro Goes
Data de Publicação: 2016
Outros Autores: Peter Silva Rocha, Aline r. s. Diniz, Pedro Henrique Nascimento de Aguiar, Carlos Renato Machado, Leda Quercia Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1371/journal.pntd.0004555
http://hdl.handle.net/1843/55303
https://orcid.org/0000-0002-4942-5021
https://orcid.org/0000-0002-8724-3165
https://orcid.org/0000-0001-5481-9618
Resumo: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
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spelling 2023-06-23T22:46:12Z2023-06-23T22:46:12Z2016104https://doi.org/10.1371/journal.pntd.00045551935-2735http://hdl.handle.net/1843/55303https://orcid.org/0000-0002-4942-5021https://orcid.org/0000-0002-8724-3165https://orcid.org/0000-0001-5481-9618CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorBackground: During Trypanosoma cruzi infection, macrophages produce reactive oxygen species (ROS) in a process called respiratory burst. Several works have aimed to elucidate the role of ROS during T. cruzi infection and the results obtained are sometimes contradictory. T. cruzi has a highly efficiently regulated antioxidant machinery to deal with the oxidative burst, but the parasite macromolecules, particularly DNA, may still suffer oxidative damage. Guanine (G) is the most vulnerable base and its oxidation results in formation of 8-oxoG, a cellular marker of oxidative stress. Methodology/Principal Findings: In order to investigate the contribution of ROS in T. cruzi survival and infection, we utilized mice deficient in the gp91phox (Phox KO) subunit of NADPH oxidase and parasites that overexpress the enzyme EcMutT (from Escherichia coli) or TcMTH (from T. cruzi), which is responsible for removing 8-oxo-dGTP from the nucleotide pool. The modified parasites presented enhanced replication inside murine inflammatory macrophages from C57BL/6 WT mice when compared with control parasites. Interestingly, when Phox KO macrophages were infected with these parasites, we observed a decreased number of all parasites when compared with macrophages from C57BL/6 WT. Scavengers for ROS also decreased parasite growth in WT macrophages. In addition, treatment of macrophages or parasites with hydrogen peroxide increased parasite replication in Phox KO mice and in vivo. Conclusions: Our results indicate a paradoxical role for ROS since modified parasites multiply better inside macrophages, but proliferation is significantly reduced when ROS is removed from the host cell. Our findings suggest that ROS can work like a signaling molecule, contributing to T. cruzi growth inside the cells.engUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIAPLoS Neglected Tropical DiseasesTrypanosoma cruziDoença de chagasEspécies reativas de nitrogênioEspécies reativas de oxigênioTrypanosoma cruziChagas diseaseespécies reativas de nitrogênioespécies reativas de oxigênioTrypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophagesTrypanosoma cruzi precisa de um sinal fornecido por espécies reativas de oxigênio para infectar macrófagosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004555Grazielle Ribeiro GoesPeter Silva RochaAline r. s. DinizPedro Henrique Nascimento de AguiarCarlos Renato MachadoLeda Quercia Vieiraapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/55303/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALTrypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages.pdfTrypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages.pdfapplication/pdf1569635https://repositorio.ufmg.br/bitstream/1843/55303/2/Trypanosoma%20cruzi%20needs%20a%20signal%20provided%20by%20reactive%20oxygen%20species%20to%20infect%20macrophages.pdffd51bfe9ca71361a43b69c82e66d29ebMD521843/553032023-06-23 19:46:12.901oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-06-23T22:46:12Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages
dc.title.alternative.pt_BR.fl_str_mv Trypanosoma cruzi precisa de um sinal fornecido por espécies reativas de oxigênio para infectar macrófagos
title Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages
spellingShingle Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages
Grazielle Ribeiro Goes
Trypanosoma cruzi
Chagas disease
espécies reativas de nitrogênio
espécies reativas de oxigênio
Trypanosoma cruzi
Doença de chagas
Espécies reativas de nitrogênio
Espécies reativas de oxigênio
title_short Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages
title_full Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages
title_fullStr Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages
title_full_unstemmed Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages
title_sort Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages
author Grazielle Ribeiro Goes
author_facet Grazielle Ribeiro Goes
Peter Silva Rocha
Aline r. s. Diniz
Pedro Henrique Nascimento de Aguiar
Carlos Renato Machado
Leda Quercia Vieira
author_role author
author2 Peter Silva Rocha
Aline r. s. Diniz
Pedro Henrique Nascimento de Aguiar
Carlos Renato Machado
Leda Quercia Vieira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Grazielle Ribeiro Goes
Peter Silva Rocha
Aline r. s. Diniz
Pedro Henrique Nascimento de Aguiar
Carlos Renato Machado
Leda Quercia Vieira
dc.subject.por.fl_str_mv Trypanosoma cruzi
Chagas disease
espécies reativas de nitrogênio
espécies reativas de oxigênio
topic Trypanosoma cruzi
Chagas disease
espécies reativas de nitrogênio
espécies reativas de oxigênio
Trypanosoma cruzi
Doença de chagas
Espécies reativas de nitrogênio
Espécies reativas de oxigênio
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de chagas
Espécies reativas de nitrogênio
Espécies reativas de oxigênio
description CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2023-06-23T22:46:12Z
dc.date.available.fl_str_mv 2023-06-23T22:46:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/55303
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1371/journal.pntd.0004555
dc.identifier.issn.pt_BR.fl_str_mv 1935-2735
dc.identifier.orcid.pt_BR.fl_str_mv https://orcid.org/0000-0002-4942-5021
https://orcid.org/0000-0002-8724-3165
https://orcid.org/0000-0001-5481-9618
url https://doi.org/10.1371/journal.pntd.0004555
http://hdl.handle.net/1843/55303
https://orcid.org/0000-0002-4942-5021
https://orcid.org/0000-0002-8724-3165
https://orcid.org/0000-0001-5481-9618
identifier_str_mv 1935-2735
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv PLoS Neglected Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
bitstream.url.fl_str_mv https://repositorio.ufmg.br/bitstream/1843/55303/1/License.txt
https://repositorio.ufmg.br/bitstream/1843/55303/2/Trypanosoma%20cruzi%20needs%20a%20signal%20provided%20by%20reactive%20oxygen%20species%20to%20infect%20macrophages.pdf
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