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2022-07-19T23:32:19Z2022-07-19T23:32:19Z201613110.1186/s12986-016-0152-41743-7075http://hdl.handle.net/1843/43433Background OxyElite Pro (OEP) is a dietary supplement to increase metabolism which contains as key stimulant the ingredient 1,3-dimethylamylamine (DMAA). Serious adverse effects have been reported after OEP consumption however, these effects are related to poisoning or overdose. To our knowledge, no one studied the effects of OEP at controlled doses. Thus, the aim of this study was to evaluate acute and chronic OEP affects, at controlled doses in Wistar rats, on physical performance, metabolic parameters, liver injury markers and oxidative stress markers and mitochondrial biogenesis in skeletal muscle. Methods Rats were divided in control, 4.3 mg OEP/kg, 12.9 mg OEP/kg and 25.8 mg OEP/kg. All groups were submitted to supplementation with OEP for 4 weeks and the experimental protocols were performed 30 min after the first OEP administration (acute response) and 30 min after the last OEP administration at the end of the forth week (chronic response). Results Running distance and running time increased after acute administration of 12.9 mg OEP/kg (2.6-fold) and 25.8 mg OEP/kg (2.8-fold). Since no effect on the exercise tolerance test was observed at the lower OEP dose (4.3 mg OEP/kg), this group was removed from further analyzes. On other hand, running distance and running time decreased after daily supplementation for 4 weeks also in both groups (64% in 12.9 mg OEP/kg and 72% in 25.8 mg OEP/kg). Chronic supplementation at both 12.9 and 25.8 mg OEP/kg decreased TBARS levels in soleus muscle (36 and 31%) and liver (43 and 25%). AOPP was also decreased by both doses in the liver (39 and 45%). Chronic administration of the highest dose, 25.8 mg OEP/kg, was able to reduce mRNA expression of PGC-1α in soleus muscle (25%). No effect was found in other analyses such as spontaneous physical activity, body weight, food and water intake, hepatic toxicity, cardiac oxidative stress and mitochondrial DNA amount. Conclusion Maximum and not recommended doses of OEP ingested acutely presented stimulating effect on the ability to exercise. However, its daily consumption for 4 weeks showed antioxidant effects in soleus muscle and liver which may have decreased the PGC-1α mRNA expression on soleus muscle and contributed to the impaired performance in the exercise tolerance test.Fundo OxyElite Pro (OEP) é um suplemento alimentar para aumentar o metabolismo que contém como estimulante chave o ingrediente 1,3-dimetilamilamina (DMAA). Efeitos adversos graves foram relatados após o consumo de OEP, no entanto, esses efeitos estão relacionados a envenenamento ou overdose. Até onde sabemos, ninguém estudou os efeitos da OEP em doses controladas. Assim, o objetivo deste estudo foi avaliar os efeitos agudos e crônicos da OEP, em doses controladas em ratos Wistar, no desempenho físico, parâmetros metabólicos, marcadores de lesão hepática e marcadores de estresse oxidativo e biogênese mitocondrial no músculo esquelético. Métodos Os ratos foram divididos em controle, 4,3 mg OEP/kg, 12,9 mg OEP/kg e 25,8 mg OEP/kg. Todos os grupos foram submetidos à suplementação com OEP por 4 semanas e os protocolos experimentais foram realizados 30 minutos após a primeira administração de OEP (resposta aguda) e 30 minutos após a última administração de OEP ao final da quarta semana (resposta crônica). Resultados A distância e o tempo de corrida aumentaram após a administração aguda de 12,9 mg OEP/kg (2,6 vezes) e 25,8 mg OEP/kg (2,8 vezes). Como nenhum efeito no teste de tolerância ao exercício foi observado na dose mais baixa de OEP (4,3 mg OEP/kg), esse grupo foi removido de análises posteriores. Por outro lado, a distância percorrida e o tempo de corrida diminuíram após a suplementação diária por 4 semanas também em ambos os grupos (64% em 12,9 mg OEP/kg e 72% em 25,8 mg OEP/kg). A suplementação crônica de 12,9 e 25,8 mg OEP/kg diminuiu os níveis de TBARS no músculo sóleo (36 e 31%) e no fígado (43 e 25%). AOPP também foi diminuída por ambas as doses no fígado (39 e 45%). A administração crônica da maior dose, 25,8 mg OEP/kg, foi capaz de reduzir a expressão de mRNA de PGC-1α no músculo sóleo (25%). Nenhum efeito foi encontrado em outras análises, como atividade física espontânea, peso corporal, ingestão de alimentos e água, toxicidade hepática, estresse oxidativo cardíaco e quantidade de DNA mitocondrial. Conclusão Doses máximas e não recomendadas de OEP ingeridas de forma aguda apresentaram efeito estimulante sobre a capacidade de exercício. No entanto, seu consumo diário por 4 semanas mostrou efeitos antioxidantes no músculo sóleo e fígado, o que pode ter diminuído a expressão do mRNA de PGC-1α no músculo sóleo e contribuído para o desempenho prejudicado no teste de tolerância ao exercício.engUniversidade Federal de Minas GeraisUFMGBrasilMEDICINA - FACULDADE DE MEDICINANutrition & metabolismSuplementos dietéticosDesempenhoFígadoEstresse oxidativoBiogênese mitocondrialEffects of controlled doses of oxyelite pro on physical performance in ratsEfeitos de doses controladas de oxyelite pro no desempenho físico em ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://nutritionandmetabolism.biomedcentral.com/articles/10.1186/s12986-016-0152-4Paulo Vinicios CamuzizovicoValério Garrone BaraunaVictor Magalhães CurtyMarcos André Soares LealEduardo Frizzera MeiraDaniel Ventura DiasLívia Carla de Melo RodriguesSilvana Dos Santos MeyrellesEdilamar Menezes de OliveiraPaula Frizera Vassalloapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/43433/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINAL2016_Effects of controlled doses of oxyelite pro on physical performance in rats.pdf2016_Effects of controlled doses of oxyelite pro on physical performance in rats.pdfapplication/pdf803177https://repositorio.ufmg.br/bitstream/1843/43433/2/2016_Effects%20of%20controlled%20doses%20of%20oxyelite%20pro%20on%20physical%20performance%20in%20rats.pdfd388c6aa974e81603bc7e39e7df274f8MD521843/434332022-07-19 20:32:20.045oai:repositorio.ufmg.br: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Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-07-19T23:32:20Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
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