Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.1186/s12951-017-0259-4 http://hdl.handle.net/1843/42419 https://orcid.org/0000-0003-1378-5380 https://orcid.org/0000-0002-5978-2735 https://orcid.org/0000-0002-2854-0768 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2022-06-10T14:52:43Z2022-06-10T14:52:43Z2017-04-0415113https://doi.org/10.1186/s12951-017-0259-41477-3155http://hdl.handle.net/1843/42419https://orcid.org/0000-0003-1378-5380https://orcid.org/0000-0002-5978-2735https://orcid.org/0000-0002-2854-0768CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorBackground: Dengue is the most prevalent arthropod‑borne viral disease in the world. In this article we present results on the development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. Methods: The MWNT‑DENV3E nanoconjugate was developed by covalent functionalization of carboxylated multi‑walled carbon nanotubes (MWNT) with recombinant dengue envelope (DENV3E) proteins. The recombinant antigens were bound to the MWNT using a diimide‑activated amidation process and the immunogen was characterized by TEM, AFM and Raman Spectroscopy. Furthermore, the immunogenicity of this vaccine candidate was evaluated in a murine model. Results: Immunization with MWNT‑DENV3E induced comparable IgG responses in relation to the immunization with non‑conjugated proteins; however, the inoculation of the nanoconjugate into mice generated higher titers of neutralizing antibodies. Cell‑mediated responses were also evaluated, and higher dengue‑specific splenocyte proliferation was observed in cell cultures derived from mice immunized with MWNT‑DENV3E when compared to animals immu‑ nized with the non‑conjugated DENV3E. Conclusions: Despite the recent licensure of the CYD‑TDV dengue vaccine in some countries, results from the vaccine’s phase III trial have cast doubts about its overall efficacy and global applicability. While questions about the effectiveness of the CYD‑TDV vaccine still lingers, it is wise to keep at hand an array of vaccine candidates, including alternative non‑classical approaches like the one presented here.engUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE MICROBIOLOGIAICB - DEPARTAMENTO DE PATOLOGIAICX - DEPARTAMENTO DE FÍSICAVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVAJournal of NanobiotechnologyNanotubos de carbonoEspectroscopia de RamanDengueDengue vaccineCarbon nanotubesSubunit vaccineNanoconjugateMulti-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-017-0259-4Alice Freitas VersianiAdo Jorio de VasconcelosFlávio Guimarães da FonsecaRuiz Gerhardt AstigarragaEliseu Soares de Oliveira RochaAna Paula Moreira BarbozaErna Geessien KroonMilene Alvarenga RachidDaniele da Glória de SouzaLuiz Orlando LadeiraEdel Figueiredo Barbosa Stancioliapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/42419/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALMulti-walled carbon nanotubes.pdfMulti-walled carbon nanotubes.pdfapplication/pdf7409281https://repositorio.ufmg.br/bitstream/1843/42419/2/Multi-walled%20carbon%20nanotubes.pdfcd7ddd51065c201051b81c1e14abb732MD521843/424192022-06-10 11:52:44.144oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-06-10T14:52:44Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
| title |
Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
| spellingShingle |
Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice Alice Freitas Versiani Dengue vaccine Carbon nanotubes Subunit vaccine Nanoconjugate Nanotubos de carbono Espectroscopia de Raman Dengue |
| title_short |
Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
| title_full |
Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
| title_fullStr |
Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
| title_full_unstemmed |
Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
| title_sort |
Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
| author |
Alice Freitas Versiani |
| author_facet |
Alice Freitas Versiani Ado Jorio de Vasconcelos Flávio Guimarães da Fonseca Ruiz Gerhardt Astigarraga Eliseu Soares de Oliveira Rocha Ana Paula Moreira Barboza Erna Geessien Kroon Milene Alvarenga Rachid Daniele da Glória de Souza Luiz Orlando Ladeira Edel Figueiredo Barbosa Stancioli |
| author_role |
author |
| author2 |
Ado Jorio de Vasconcelos Flávio Guimarães da Fonseca Ruiz Gerhardt Astigarraga Eliseu Soares de Oliveira Rocha Ana Paula Moreira Barboza Erna Geessien Kroon Milene Alvarenga Rachid Daniele da Glória de Souza Luiz Orlando Ladeira Edel Figueiredo Barbosa Stancioli |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Alice Freitas Versiani Ado Jorio de Vasconcelos Flávio Guimarães da Fonseca Ruiz Gerhardt Astigarraga Eliseu Soares de Oliveira Rocha Ana Paula Moreira Barboza Erna Geessien Kroon Milene Alvarenga Rachid Daniele da Glória de Souza Luiz Orlando Ladeira Edel Figueiredo Barbosa Stancioli |
| dc.subject.por.fl_str_mv |
Dengue vaccine Carbon nanotubes Subunit vaccine Nanoconjugate |
| topic |
Dengue vaccine Carbon nanotubes Subunit vaccine Nanoconjugate Nanotubos de carbono Espectroscopia de Raman Dengue |
| dc.subject.other.pt_BR.fl_str_mv |
Nanotubos de carbono Espectroscopia de Raman Dengue |
| description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| publishDate |
2017 |
| dc.date.issued.fl_str_mv |
2017-04-04 |
| dc.date.accessioned.fl_str_mv |
2022-06-10T14:52:43Z |
| dc.date.available.fl_str_mv |
2022-06-10T14:52:43Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/1843/42419 |
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https://doi.org/10.1186/s12951-017-0259-4 |
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1477-3155 |
| dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0003-1378-5380 https://orcid.org/0000-0002-5978-2735 https://orcid.org/0000-0002-2854-0768 |
| url |
https://doi.org/10.1186/s12951-017-0259-4 http://hdl.handle.net/1843/42419 https://orcid.org/0000-0003-1378-5380 https://orcid.org/0000-0002-5978-2735 https://orcid.org/0000-0002-2854-0768 |
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1477-3155 |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Journal of Nanobiotechnology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - DEPARTAMENTO DE MICROBIOLOGIA ICB - DEPARTAMENTO DE PATOLOGIA ICX - DEPARTAMENTO DE FÍSICA VET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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Universidade Federal de Minas Gerais (UFMG) |
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