Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice

Detalhes bibliográficos
Autor(a) principal: Alice Freitas Versiani
Data de Publicação: 2017
Outros Autores: Ado Jorio de Vasconcelos, Flávio Guimarães da Fonseca, Ruiz Gerhardt Astigarraga, Eliseu Soares de Oliveira Rocha, Ana Paula Moreira Barboza, Erna Geessien Kroon, Milene Alvarenga Rachid, Daniele da Glória de Souza, Luiz Orlando Ladeira, Edel Figueiredo Barbosa Stancioli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1186/s12951-017-0259-4
http://hdl.handle.net/1843/42419
https://orcid.org/0000-0003-1378-5380
https://orcid.org/0000-0002-5978-2735
https://orcid.org/0000-0002-2854-0768
Resumo: Background: Dengue is the most prevalent arthropod‑borne viral disease in the world. In this article we present results on the development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. Methods: The MWNT‑DENV3E nanoconjugate was developed by covalent functionalization of carboxylated multi‑walled carbon nanotubes (MWNT) with recombinant dengue envelope (DENV3E) proteins. The recombinant antigens were bound to the MWNT using a diimide‑activated amidation process and the immunogen was characterized by TEM, AFM and Raman Spectroscopy. Furthermore, the immunogenicity of this vaccine candidate was evaluated in a murine model. Results: Immunization with MWNT‑DENV3E induced comparable IgG responses in relation to the immunization with non‑conjugated proteins; however, the inoculation of the nanoconjugate into mice generated higher titers of neutralizing antibodies. Cell‑mediated responses were also evaluated, and higher dengue‑specific splenocyte proliferation was observed in cell cultures derived from mice immunized with MWNT‑DENV3E when compared to animals immu‑ nized with the non‑conjugated DENV3E. Conclusions: Despite the recent licensure of the CYD‑TDV dengue vaccine in some countries, results from the vaccine’s phase III trial have cast doubts about its overall efficacy and global applicability. While questions about the effectiveness of the CYD‑TDV vaccine still lingers, it is wise to keep at hand an array of vaccine candidates, including alternative non‑classical approaches like the one presented here.
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spelling Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in miceDengue vaccineCarbon nanotubesSubunit vaccineNanoconjugateNanotubos de carbonoEspectroscopia de RamanDengueBackground: Dengue is the most prevalent arthropod‑borne viral disease in the world. In this article we present results on the development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. Methods: The MWNT‑DENV3E nanoconjugate was developed by covalent functionalization of carboxylated multi‑walled carbon nanotubes (MWNT) with recombinant dengue envelope (DENV3E) proteins. The recombinant antigens were bound to the MWNT using a diimide‑activated amidation process and the immunogen was characterized by TEM, AFM and Raman Spectroscopy. Furthermore, the immunogenicity of this vaccine candidate was evaluated in a murine model. Results: Immunization with MWNT‑DENV3E induced comparable IgG responses in relation to the immunization with non‑conjugated proteins; however, the inoculation of the nanoconjugate into mice generated higher titers of neutralizing antibodies. Cell‑mediated responses were also evaluated, and higher dengue‑specific splenocyte proliferation was observed in cell cultures derived from mice immunized with MWNT‑DENV3E when compared to animals immu‑ nized with the non‑conjugated DENV3E. Conclusions: Despite the recent licensure of the CYD‑TDV dengue vaccine in some countries, results from the vaccine’s phase III trial have cast doubts about its overall efficacy and global applicability. While questions about the effectiveness of the CYD‑TDV vaccine still lingers, it is wise to keep at hand an array of vaccine candidates, including alternative non‑classical approaches like the one presented here.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal de Minas GeraisBrasilICB - DEPARTAMENTO DE MICROBIOLOGIAICB - DEPARTAMENTO DE PATOLOGIAICX - DEPARTAMENTO DE FÍSICAVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVAUFMG2022-06-10T14:52:43Z2022-06-10T14:52:43Z2017-04-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdfhttps://doi.org/10.1186/s12951-017-0259-41477-3155http://hdl.handle.net/1843/42419https://orcid.org/0000-0003-1378-5380https://orcid.org/0000-0002-5978-2735https://orcid.org/0000-0002-2854-0768engJournal of NanobiotechnologyAlice Freitas VersianiAdo Jorio de VasconcelosFlávio Guimarães da FonsecaRuiz Gerhardt AstigarragaEliseu Soares de Oliveira RochaAna Paula Moreira BarbozaErna Geessien KroonMilene Alvarenga RachidDaniele da Glória de SouzaLuiz Orlando LadeiraEdel Figueiredo Barbosa Stancioliinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2022-06-10T14:52:44Zoai:repositorio.ufmg.br:1843/42419Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2022-06-10T14:52:44Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
title Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
spellingShingle Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
Alice Freitas Versiani
Dengue vaccine
Carbon nanotubes
Subunit vaccine
Nanoconjugate
Nanotubos de carbono
Espectroscopia de Raman
Dengue
title_short Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
title_full Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
title_fullStr Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
title_full_unstemmed Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
title_sort Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
author Alice Freitas Versiani
author_facet Alice Freitas Versiani
Ado Jorio de Vasconcelos
Flávio Guimarães da Fonseca
Ruiz Gerhardt Astigarraga
Eliseu Soares de Oliveira Rocha
Ana Paula Moreira Barboza
Erna Geessien Kroon
Milene Alvarenga Rachid
Daniele da Glória de Souza
Luiz Orlando Ladeira
Edel Figueiredo Barbosa Stancioli
author_role author
author2 Ado Jorio de Vasconcelos
Flávio Guimarães da Fonseca
Ruiz Gerhardt Astigarraga
Eliseu Soares de Oliveira Rocha
Ana Paula Moreira Barboza
Erna Geessien Kroon
Milene Alvarenga Rachid
Daniele da Glória de Souza
Luiz Orlando Ladeira
Edel Figueiredo Barbosa Stancioli
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alice Freitas Versiani
Ado Jorio de Vasconcelos
Flávio Guimarães da Fonseca
Ruiz Gerhardt Astigarraga
Eliseu Soares de Oliveira Rocha
Ana Paula Moreira Barboza
Erna Geessien Kroon
Milene Alvarenga Rachid
Daniele da Glória de Souza
Luiz Orlando Ladeira
Edel Figueiredo Barbosa Stancioli
dc.subject.por.fl_str_mv Dengue vaccine
Carbon nanotubes
Subunit vaccine
Nanoconjugate
Nanotubos de carbono
Espectroscopia de Raman
Dengue
topic Dengue vaccine
Carbon nanotubes
Subunit vaccine
Nanoconjugate
Nanotubos de carbono
Espectroscopia de Raman
Dengue
description Background: Dengue is the most prevalent arthropod‑borne viral disease in the world. In this article we present results on the development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. Methods: The MWNT‑DENV3E nanoconjugate was developed by covalent functionalization of carboxylated multi‑walled carbon nanotubes (MWNT) with recombinant dengue envelope (DENV3E) proteins. The recombinant antigens were bound to the MWNT using a diimide‑activated amidation process and the immunogen was characterized by TEM, AFM and Raman Spectroscopy. Furthermore, the immunogenicity of this vaccine candidate was evaluated in a murine model. Results: Immunization with MWNT‑DENV3E induced comparable IgG responses in relation to the immunization with non‑conjugated proteins; however, the inoculation of the nanoconjugate into mice generated higher titers of neutralizing antibodies. Cell‑mediated responses were also evaluated, and higher dengue‑specific splenocyte proliferation was observed in cell cultures derived from mice immunized with MWNT‑DENV3E when compared to animals immu‑ nized with the non‑conjugated DENV3E. Conclusions: Despite the recent licensure of the CYD‑TDV dengue vaccine in some countries, results from the vaccine’s phase III trial have cast doubts about its overall efficacy and global applicability. While questions about the effectiveness of the CYD‑TDV vaccine still lingers, it is wise to keep at hand an array of vaccine candidates, including alternative non‑classical approaches like the one presented here.
publishDate 2017
dc.date.none.fl_str_mv 2017-04-04
2022-06-10T14:52:43Z
2022-06-10T14:52:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1186/s12951-017-0259-4
1477-3155
http://hdl.handle.net/1843/42419
https://orcid.org/0000-0003-1378-5380
https://orcid.org/0000-0002-5978-2735
https://orcid.org/0000-0002-2854-0768
url https://doi.org/10.1186/s12951-017-0259-4
http://hdl.handle.net/1843/42419
https://orcid.org/0000-0003-1378-5380
https://orcid.org/0000-0002-5978-2735
https://orcid.org/0000-0002-2854-0768
identifier_str_mv 1477-3155
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Nanobiotechnology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
Brasil
ICB - DEPARTAMENTO DE MICROBIOLOGIA
ICB - DEPARTAMENTO DE PATOLOGIA
ICX - DEPARTAMENTO DE FÍSICA
VET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA
UFMG
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
Brasil
ICB - DEPARTAMENTO DE MICROBIOLOGIA
ICB - DEPARTAMENTO DE PATOLOGIA
ICX - DEPARTAMENTO DE FÍSICA
VET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA
UFMG
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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