Identification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.4238/gmr18521 http://hdl.handle.net/1843/42229 https://orcid.org/ 0000-0001-9836-4117 |
Resumo: | FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais |
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2022-06-03T15:57:27Z2022-06-03T15:57:27Z2020191https://doi.org/10.4238/gmr185211676-5680http://hdl.handle.net/1843/42229https://orcid.org/ 0000-0001-9836-4117FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisPigeon droppings carry microorganisms such as Candida spp., which have high genetic variability and are the most common opportunistic pathogens in humans. There is a need to establish fast, reliable and simple laboratory methods to differentiate pathogenic from non-pathogenic yeast species in pigeon droppings, as they can be a source of contamination for humans. We examined yeasts isolated from domestic pigeon droppings through a proteomic approach using matrix-assisted laser desorption ionization (MALDI-TOF). We also examined genetic characteristics of the isolates by restriction fragment length polymorphism (RFLP) PCR and enterobacterial repetitive intergenic consensus sequences PCR methods. Five colonies isolated from domestic pigeon droppings collected at three different sampling locations on the roof of a Brazilian university building were identified as Candida krusei and Candida rugosa by MALDI-TOF. Phylogenetic studies using the two PCR-based molecular methods were able to differentiate the species C. krusei and C. rugosa into different groups. These genomic methods gave similar results and proved useful for genetic diversity analysis and interspecific differentiation of Candida isolates.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASGenetics and Molecular ResearchLevedosFilogeniaIdentification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.geneticsmr.com/articles/identification-and-molecular-analysis-yeasts-found-domestic-pigeon-droppings-montes-clarosJosiane dos SantosAlessandra Rejane Ericsson de Oliveira XavierMauro Aparecido de Sousa XavierLéia CardosoSérgio Avelino Mota NobreRicardo Rodrigues BacchiCarlos Humbertp Chamone CangussuAnna Christina de AlmeidaLuciana Nobre LeiteNair Amélia Prates Barretoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/42229/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALIdentification and molecular analysis yeasts found domestic pigeon droppings Montes Claros, MG, Brazil.pdfIdentification and molecular analysis yeasts found domestic pigeon droppings Montes Claros, MG, Brazil.pdfapplication/pdf849870https://repositorio.ufmg.br/bitstream/1843/42229/2/Identification%20and%20molecular%20analysis%20yeasts%20found%20domestic%20pigeon%20droppings%20Montes%20Claros%2c%20MG%2c%20Brazil.pdfcd58b5a628f211f048f9fca6f0a83dd9MD521843/422292022-06-03 12:57:27.627oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-06-03T15:57:27Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Identification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil. |
| title |
Identification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil. |
| spellingShingle |
Identification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil. Josiane dos Santos Levedos Filogenia |
| title_short |
Identification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil. |
| title_full |
Identification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil. |
| title_fullStr |
Identification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil. |
| title_full_unstemmed |
Identification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil. |
| title_sort |
Identification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil. |
| author |
Josiane dos Santos |
| author_facet |
Josiane dos Santos Alessandra Rejane Ericsson de Oliveira Xavier Mauro Aparecido de Sousa Xavier Léia Cardoso Sérgio Avelino Mota Nobre Ricardo Rodrigues Bacchi Carlos Humbertp Chamone Cangussu Anna Christina de Almeida Luciana Nobre Leite Nair Amélia Prates Barreto |
| author_role |
author |
| author2 |
Alessandra Rejane Ericsson de Oliveira Xavier Mauro Aparecido de Sousa Xavier Léia Cardoso Sérgio Avelino Mota Nobre Ricardo Rodrigues Bacchi Carlos Humbertp Chamone Cangussu Anna Christina de Almeida Luciana Nobre Leite Nair Amélia Prates Barreto |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Josiane dos Santos Alessandra Rejane Ericsson de Oliveira Xavier Mauro Aparecido de Sousa Xavier Léia Cardoso Sérgio Avelino Mota Nobre Ricardo Rodrigues Bacchi Carlos Humbertp Chamone Cangussu Anna Christina de Almeida Luciana Nobre Leite Nair Amélia Prates Barreto |
| dc.subject.other.pt_BR.fl_str_mv |
Levedos Filogenia |
| topic |
Levedos Filogenia |
| description |
FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais |
| publishDate |
2020 |
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2020 |
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2022-06-03T15:57:27Z |
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2022-06-03T15:57:27Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://hdl.handle.net/1843/42229 |
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https://doi.org/10.4238/gmr18521 |
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1676-5680 |
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https://orcid.org/ 0000-0001-9836-4117 |
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https://doi.org/10.4238/gmr18521 http://hdl.handle.net/1843/42229 https://orcid.org/ 0000-0001-9836-4117 |
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eng |
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Genetics and Molecular Research |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
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Universidade Federal de Minas Gerais |
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