Natural concurrent infections associated with infectious laryngotracheitis in layer chickens
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.3382/japr/pfv075 http://hdl.handle.net/1843/67350 https://orcid.org/0000-0001-5907-3798 https://orcid.org/0000-0002-9374-5591 https://orcid.org/0000-0001-8925-2228 https://orcid.org/0000-0002-8052-5389 |
Resumo: | Layer chickens with severe respiratory disease belonging to farms with chickens infected by gallid herpesvirus (GaHV-1) were evaluated. Samples of nasal turbinates/sinuses and trachea from 31 chickens were subjected to histopathological analysis, which showed that 22.6% of chickens had lesions suggestive of co-infection, most frequently by GaHV-1 or Mycoplasma spp. PCR analysis showed the presence of at least two respiratory pathogens in 61.2% of chickens. Partial sequencing was performed for identification of the intergenic spacer region (IGSR) and cytadhesin 2 (mgc2) gene of the Mycoplasma gallisepticum genome. Analysis of IGSR revealed the involvement of two field and one vaccine strains. However, analysis of mgc2 from the same samples revealed five different strains of M. gallisepticum, four of which were field strains and one of which was a vaccine strain. These results demonstrate that combined molecular and histopathological analysis is a reliable and conclusive method for diagnosis of respiratory diseases in chickens since some etiologic agents can remain latent or maintain chickens as carriers. The results further confirm the importance and feasibility of the molecular characterization of M. gallisepticum genes to differentiate between vaccine and field strains involved in cases of respiratory disease in chickens. |
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2024-04-17T20:34:17Z2024-04-17T20:34:17Z201625113128https://doi.org/10.3382/japr/pfv0751537-0437http://hdl.handle.net/1843/67350https://orcid.org/0000-0001-5907-3798https://orcid.org/0000-0002-9374-5591https://orcid.org/0000-0001-8925-2228https://orcid.org/0000-0002-8052-5389Layer chickens with severe respiratory disease belonging to farms with chickens infected by gallid herpesvirus (GaHV-1) were evaluated. Samples of nasal turbinates/sinuses and trachea from 31 chickens were subjected to histopathological analysis, which showed that 22.6% of chickens had lesions suggestive of co-infection, most frequently by GaHV-1 or Mycoplasma spp. PCR analysis showed the presence of at least two respiratory pathogens in 61.2% of chickens. Partial sequencing was performed for identification of the intergenic spacer region (IGSR) and cytadhesin 2 (mgc2) gene of the Mycoplasma gallisepticum genome. Analysis of IGSR revealed the involvement of two field and one vaccine strains. However, analysis of mgc2 from the same samples revealed five different strains of M. gallisepticum, four of which were field strains and one of which was a vaccine strain. These results demonstrate that combined molecular and histopathological analysis is a reliable and conclusive method for diagnosis of respiratory diseases in chickens since some etiologic agents can remain latent or maintain chickens as carriers. The results further confirm the importance and feasibility of the molecular characterization of M. gallisepticum genes to differentiate between vaccine and field strains involved in cases of respiratory disease in chickens.Foram avaliadas galinhas poedeiras com doença respiratória grave pertencentes a granjas com galinhas infectadas pelo herpesvírus de galídeos (GaHV-1). Amostras de conchas/seios nasais e traqueia de 31 galinhas foram submetidas à análise histopatológica, que mostrou que 22,6% das galinhas apresentavam lesões sugestivas de coinfecção, mais frequentemente por GaHV-1 ou Mycoplasma spp. A análise de PCR mostrou a presença de pelo menos dois patógenos respiratórios em 61,2% das galinhas. O sequenciamento parcial foi realizado para identificação da região espaçadora intergênica (IGSR) e do gene citadesina 2 (mgc2) do genoma de Mycoplasma gallisepticum. A análise do IGSR revelou o envolvimento de duas cepas de campo e uma cepa vacinal. Contudo, a análise de mgc2 das mesmas amostras revelou cinco estirpes diferentes de M. gallisepticum, quatro das quais eram estirpes de campo e uma das quais era uma estirpe vacinal. Estes resultados demonstram que a análise molecular e histopatológica combinada é um método confiável e conclusivo para o diagnóstico de doenças respiratórias em galinhas, uma vez que alguns agentes etiológicos podem permanecer latentes ou manter as galinhas como portadoras. Os resultados confirmam ainda a importância e viabilidade da caracterização molecular dos genes de M. gallisepticum para diferenciar entre cepas vacinais e de campo envolvidas em casos de doenças respiratórias em galinhas.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorengUniversidade Federal de Minas GeraisUFMGBrasilVET - DEPARTAMENTO DE CLÍNICA E CIRURGIAVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVAJournal of applied poultry researchGalinhas - ImunologiaDoenças respiratóriasHerpesvirus galináceo 1Mycoplasma hyopneumoniaeDNA IntergênicoHistopatologia veterinariaLayer chickenRespiratory diseaseGaHV-1Mycoplasma sppMgc2IGSRHistopathologyNatural concurrent infections associated with infectious laryngotracheitis in layer chickensInfecções concomitantes naturais associadas à laringotraqueíte infecciosa em galinhas poedeirasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S1056617119302156Rodrigo M. CoutoJuliana F. Vilarinho BragaSandra Yuliet MarinMauricio ResendeNelson Rodrigo da Silva MartinsRoselene Eccoapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/67350/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALNatural concurrent infections associated with infectious laryngotracheitis in layer chickens.pdfNatural concurrent infections associated with infectious laryngotracheitis in layer chickens.pdfapplication/pdf1328349https://repositorio.ufmg.br/bitstream/1843/67350/2/Natural%20concurrent%20infections%20associated%20with%20infectious%20laryngotracheitis%20in%20layer%20chickens.pdfb21a46dad717c9866f5cad53c8d5ac21MD521843/673502024-04-17 17:34:17.791oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2024-04-17T20:34:17Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Natural concurrent infections associated with infectious laryngotracheitis in layer chickens |
dc.title.alternative.pt_BR.fl_str_mv |
Infecções concomitantes naturais associadas à laringotraqueíte infecciosa em galinhas poedeiras |
title |
Natural concurrent infections associated with infectious laryngotracheitis in layer chickens |
spellingShingle |
Natural concurrent infections associated with infectious laryngotracheitis in layer chickens Rodrigo M. Couto Layer chicken Respiratory disease GaHV-1 Mycoplasma spp Mgc2 IGSR Histopathology Galinhas - Imunologia Doenças respiratórias Herpesvirus galináceo 1 Mycoplasma hyopneumoniae DNA Intergênico Histopatologia veterinaria |
title_short |
Natural concurrent infections associated with infectious laryngotracheitis in layer chickens |
title_full |
Natural concurrent infections associated with infectious laryngotracheitis in layer chickens |
title_fullStr |
Natural concurrent infections associated with infectious laryngotracheitis in layer chickens |
title_full_unstemmed |
Natural concurrent infections associated with infectious laryngotracheitis in layer chickens |
title_sort |
Natural concurrent infections associated with infectious laryngotracheitis in layer chickens |
author |
Rodrigo M. Couto |
author_facet |
Rodrigo M. Couto Juliana F. Vilarinho Braga Sandra Yuliet Marin Mauricio Resende Nelson Rodrigo da Silva Martins Roselene Ecco |
author_role |
author |
author2 |
Juliana F. Vilarinho Braga Sandra Yuliet Marin Mauricio Resende Nelson Rodrigo da Silva Martins Roselene Ecco |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigo M. Couto Juliana F. Vilarinho Braga Sandra Yuliet Marin Mauricio Resende Nelson Rodrigo da Silva Martins Roselene Ecco |
dc.subject.por.fl_str_mv |
Layer chicken Respiratory disease GaHV-1 Mycoplasma spp Mgc2 IGSR Histopathology |
topic |
Layer chicken Respiratory disease GaHV-1 Mycoplasma spp Mgc2 IGSR Histopathology Galinhas - Imunologia Doenças respiratórias Herpesvirus galináceo 1 Mycoplasma hyopneumoniae DNA Intergênico Histopatologia veterinaria |
dc.subject.other.pt_BR.fl_str_mv |
Galinhas - Imunologia Doenças respiratórias Herpesvirus galináceo 1 Mycoplasma hyopneumoniae DNA Intergênico Histopatologia veterinaria |
description |
Layer chickens with severe respiratory disease belonging to farms with chickens infected by gallid herpesvirus (GaHV-1) were evaluated. Samples of nasal turbinates/sinuses and trachea from 31 chickens were subjected to histopathological analysis, which showed that 22.6% of chickens had lesions suggestive of co-infection, most frequently by GaHV-1 or Mycoplasma spp. PCR analysis showed the presence of at least two respiratory pathogens in 61.2% of chickens. Partial sequencing was performed for identification of the intergenic spacer region (IGSR) and cytadhesin 2 (mgc2) gene of the Mycoplasma gallisepticum genome. Analysis of IGSR revealed the involvement of two field and one vaccine strains. However, analysis of mgc2 from the same samples revealed five different strains of M. gallisepticum, four of which were field strains and one of which was a vaccine strain. These results demonstrate that combined molecular and histopathological analysis is a reliable and conclusive method for diagnosis of respiratory diseases in chickens since some etiologic agents can remain latent or maintain chickens as carriers. The results further confirm the importance and feasibility of the molecular characterization of M. gallisepticum genes to differentiate between vaccine and field strains involved in cases of respiratory disease in chickens. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2024-04-17T20:34:17Z |
dc.date.available.fl_str_mv |
2024-04-17T20:34:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/67350 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.3382/japr/pfv075 |
dc.identifier.issn.pt_BR.fl_str_mv |
1537-0437 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0001-5907-3798 https://orcid.org/0000-0002-9374-5591 https://orcid.org/0000-0001-8925-2228 https://orcid.org/0000-0002-8052-5389 |
url |
https://doi.org/10.3382/japr/pfv075 http://hdl.handle.net/1843/67350 https://orcid.org/0000-0001-5907-3798 https://orcid.org/0000-0002-9374-5591 https://orcid.org/0000-0001-8925-2228 https://orcid.org/0000-0002-8052-5389 |
identifier_str_mv |
1537-0437 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of applied poultry research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA VET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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