Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1590/0074-02760180566 http://hdl.handle.net/1843/56308 https://orcid.org/0000-0001-8068-1720 https://orcid.org/0000-0002-5279-5621 https://orcid.org/0000-0003-1877-2570 https://orcid.org/0000-0002-1108-5666 https://orcid.org/0000-0002-1542-897X |
Resumo: | BACKGROUND: Opportunistic pathogenic yeast species are frequently associated with water habitats that have pollution sources of human or animal origin. Candida albicans has already been suggested as a faecal indicator microorganism for aquatic environments. OBJECTIVES:The goal of this study was to investigate the occurrence of C. albicans and other opportunistic yeasts in sand and seawater samples from beaches in Brazil to assess their correlation with Escherichia coli, and to characterise the pathogenic potential of the yeast isolates. METHODS: Opportunistic species (yeasts that grow at 37ºC) were isolated from sand and seawater samples from eight beaches in Brazil during the summer and the winter. Opportunistic yeast species were evaluated for their susceptibility to antifungal drugs, virulence factors, and the in vitro and in vivo biofilm formation. Strains were selected to carry out virulence tests using BALB/c mice. FINDINGS: Several water samples could be classified as inappropriate for primary contact recreation in relation to E. coli densities. C. albicans was isolated in low densities. Of the 144 opportunistic yeasts evaluated, 61% displayed resistance or dose-dependent sensitivity to at least one tested drug, and 40% produced proteinase. Strains of C. albicans and Kodamaea ohmeri exhibited the highest rates of adhesion to buccal epithelial cells. All the C. albicans strains that were tested were able to undergo morphogenesis and form a biofilm on catheter fragments in both in vitro and in vivo experiments. It was possible to confirm the pathogenic potential of three of these strains during the disseminated infection test. MAIN CONCLUSIONS: The identification of opportunistic yeast species in seawater and sand samples from Brazilian beaches suggest a potential risk to the health of people who use these environments for recreational purposes. |
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2023-07-14T20:39:43Z2023-07-14T20:39:43Z2019114https://doi.org/10.1590/0074-027601805661678-8060http://hdl.handle.net/1843/56308https://orcid.org/0000-0001-8068-1720https://orcid.org/0000-0002-5279-5621https://orcid.org/0000-0003-1877-2570https://orcid.org/0000-0002-1108-5666https://orcid.org/0000-0002-1542-897XBACKGROUND: Opportunistic pathogenic yeast species are frequently associated with water habitats that have pollution sources of human or animal origin. Candida albicans has already been suggested as a faecal indicator microorganism for aquatic environments. OBJECTIVES:The goal of this study was to investigate the occurrence of C. albicans and other opportunistic yeasts in sand and seawater samples from beaches in Brazil to assess their correlation with Escherichia coli, and to characterise the pathogenic potential of the yeast isolates. METHODS: Opportunistic species (yeasts that grow at 37ºC) were isolated from sand and seawater samples from eight beaches in Brazil during the summer and the winter. Opportunistic yeast species were evaluated for their susceptibility to antifungal drugs, virulence factors, and the in vitro and in vivo biofilm formation. Strains were selected to carry out virulence tests using BALB/c mice. FINDINGS: Several water samples could be classified as inappropriate for primary contact recreation in relation to E. coli densities. C. albicans was isolated in low densities. Of the 144 opportunistic yeasts evaluated, 61% displayed resistance or dose-dependent sensitivity to at least one tested drug, and 40% produced proteinase. Strains of C. albicans and Kodamaea ohmeri exhibited the highest rates of adhesion to buccal epithelial cells. All the C. albicans strains that were tested were able to undergo morphogenesis and form a biofilm on catheter fragments in both in vitro and in vivo experiments. It was possible to confirm the pathogenic potential of three of these strains during the disseminated infection test. MAIN CONCLUSIONS: The identification of opportunistic yeast species in seawater and sand samples from Brazilian beaches suggest a potential risk to the health of people who use these environments for recreational purposes.INTRODUÇÃO: Espécies de leveduras patogênicas oportunistas são frequentemente associadas a habitats aquáticos que possuem fontes de poluição de origem humana ou animal. A Candida albicans já foi sugerida como um microrganismo fecal indicador para ambientes aquáticos. OBJETIVOS: O objetivo deste estudo foi investigar a ocorrência de C. albicans e outras leveduras oportunistas em amostras de areia e água do mar de praias do Brasil para avaliar sua correlação com Escherichia coli e caracterizar o potencial patogênico dos isolados de levedura. MÉTODOS: Espécies oportunistas (leveduras que crescem a 37ºC) foram isoladas de amostras de areia e água do mar de oito praias do Brasil durante o verão e o inverno. Espécies de leveduras oportunistas foram avaliadas quanto à sua suscetibilidade a drogas antifúngicas, fatores de virulência e formação de biofilme in vitro e in vivo. As cepas foram selecionadas para realizar testes de virulência usando camundongos BALB/c. DADOS: Várias amostras de água podem ser classificadas como impróprias para recreação de contato primário em relação às densidades de E. coli. C. albicans foi isolado em baixas densidades. Das 144 leveduras oportunistas avaliadas, 61% apresentaram resistência ou sensibilidade dose-dependente a pelo menos uma droga testada e 40% produziram proteinase. Cepas de C. albicans e Kodamaea ohmeri exibiram as maiores taxas de adesão às células epiteliais bucais. Todas as cepas de C. albicans testadas foram capazes de sofrer morfogênese e formar um biofilme em fragmentos de cateter em ambos os experimentos in vitro e in vivo. Foi possível confirmar o potencial patogênico de três dessas cepas durante o teste de infecção disseminada. CONCLUSÕES PRINCIPAIS : A identificação de espécies de leveduras oportunistas em amostras de água do mar e areia de praias brasileiras sugere um risco potencial à saúde de pessoas que utilizam esses ambientes para fins recreativos.engUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE MICROBIOLOGIAMemórias do Instituto Oswaldo CruzEscherichia coliCandida albicansLevedurasInfecções oportunistasopportunistic pathogenic yeastsbeachesEscherichia coliCandida albicansyeast adhesiondisseminated infection testsOccurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beachesOcorrência, suscetibilidade a antifúngicos e fatores de virulência de leveduras oportunistas isoladas de praias brasileirasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/mioc/a/KTtQXRGV6hqfbTxqQGBCK9L/?lang=en#Natália O. P. MacielSusana JohannLuciana R BrandãoSona KucharíkováCamila G MoraisAlexandre P OliveiraGustavo JC FreitasBeatriz M BorelliFranciane M PellizzariDaniel de Assis SantosPatrick Van DijckCarlos A Rosaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56308/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALOccurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches .pdfOccurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches .pdfapplication/pdf10666250https://repositorio.ufmg.br/bitstream/1843/56308/2/Occurrence%2c%20antifungal%20susceptibility%2c%20and%20virulence%20factors%20of%20opportunistic%20yeasts%20isolated%20from%20Brazilian%20beaches%20.pdf5b1e7e957939a759d3815503fe8e2dd5MD521843/563082023-07-14 17:39:43.482oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-14T20:39:43Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches |
dc.title.alternative.pt_BR.fl_str_mv |
Ocorrência, suscetibilidade a antifúngicos e fatores de virulência de leveduras oportunistas isoladas de praias brasileiras |
title |
Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches |
spellingShingle |
Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches Natália O. P. Maciel opportunistic pathogenic yeasts beaches Escherichia coli Candida albicans yeast adhesion disseminated infection tests Escherichia coli Candida albicans Leveduras Infecções oportunistas |
title_short |
Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches |
title_full |
Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches |
title_fullStr |
Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches |
title_full_unstemmed |
Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches |
title_sort |
Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches |
author |
Natália O. P. Maciel |
author_facet |
Natália O. P. Maciel Susana Johann Luciana R Brandão Sona Kucharíková Camila G Morais Alexandre P Oliveira Gustavo JC Freitas Beatriz M Borelli Franciane M Pellizzari Daniel de Assis Santos Patrick Van Dijck Carlos A Rosa |
author_role |
author |
author2 |
Susana Johann Luciana R Brandão Sona Kucharíková Camila G Morais Alexandre P Oliveira Gustavo JC Freitas Beatriz M Borelli Franciane M Pellizzari Daniel de Assis Santos Patrick Van Dijck Carlos A Rosa |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Natália O. P. Maciel Susana Johann Luciana R Brandão Sona Kucharíková Camila G Morais Alexandre P Oliveira Gustavo JC Freitas Beatriz M Borelli Franciane M Pellizzari Daniel de Assis Santos Patrick Van Dijck Carlos A Rosa |
dc.subject.por.fl_str_mv |
opportunistic pathogenic yeasts beaches Escherichia coli Candida albicans yeast adhesion disseminated infection tests |
topic |
opportunistic pathogenic yeasts beaches Escherichia coli Candida albicans yeast adhesion disseminated infection tests Escherichia coli Candida albicans Leveduras Infecções oportunistas |
dc.subject.other.pt_BR.fl_str_mv |
Escherichia coli Candida albicans Leveduras Infecções oportunistas |
description |
BACKGROUND: Opportunistic pathogenic yeast species are frequently associated with water habitats that have pollution sources of human or animal origin. Candida albicans has already been suggested as a faecal indicator microorganism for aquatic environments. OBJECTIVES:The goal of this study was to investigate the occurrence of C. albicans and other opportunistic yeasts in sand and seawater samples from beaches in Brazil to assess their correlation with Escherichia coli, and to characterise the pathogenic potential of the yeast isolates. METHODS: Opportunistic species (yeasts that grow at 37ºC) were isolated from sand and seawater samples from eight beaches in Brazil during the summer and the winter. Opportunistic yeast species were evaluated for their susceptibility to antifungal drugs, virulence factors, and the in vitro and in vivo biofilm formation. Strains were selected to carry out virulence tests using BALB/c mice. FINDINGS: Several water samples could be classified as inappropriate for primary contact recreation in relation to E. coli densities. C. albicans was isolated in low densities. Of the 144 opportunistic yeasts evaluated, 61% displayed resistance or dose-dependent sensitivity to at least one tested drug, and 40% produced proteinase. Strains of C. albicans and Kodamaea ohmeri exhibited the highest rates of adhesion to buccal epithelial cells. All the C. albicans strains that were tested were able to undergo morphogenesis and form a biofilm on catheter fragments in both in vitro and in vivo experiments. It was possible to confirm the pathogenic potential of three of these strains during the disseminated infection test. MAIN CONCLUSIONS: The identification of opportunistic yeast species in seawater and sand samples from Brazilian beaches suggest a potential risk to the health of people who use these environments for recreational purposes. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2023-07-14T20:39:43Z |
dc.date.available.fl_str_mv |
2023-07-14T20:39:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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http://hdl.handle.net/1843/56308 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1590/0074-02760180566 |
dc.identifier.issn.pt_BR.fl_str_mv |
1678-8060 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0001-8068-1720 https://orcid.org/0000-0002-5279-5621 https://orcid.org/0000-0003-1877-2570 https://orcid.org/0000-0002-1108-5666 https://orcid.org/0000-0002-1542-897X |
url |
https://doi.org/10.1590/0074-02760180566 http://hdl.handle.net/1843/56308 https://orcid.org/0000-0001-8068-1720 https://orcid.org/0000-0002-5279-5621 https://orcid.org/0000-0003-1877-2570 https://orcid.org/0000-0002-1108-5666 https://orcid.org/0000-0002-1542-897X |
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1678-8060 |
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eng |
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eng |
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Memórias do Instituto Oswaldo Cruz |
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openAccess |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - DEPARTAMENTO DE MICROBIOLOGIA |
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Universidade Federal de Minas Gerais |
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