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2023-07-17T19:08:44Z2023-07-17T19:08:44Z202072622712278https://doi.org/10.1590/1678-4162-118701678-4162http://hdl.handle.net/1843/56455http://orcid.org/0000-0002-2491-3483http://orcid.org/0000-0003-0173-3524http://orcid.org/0000-0003-3870-3687http://orcid.org/0000-0002-9060-9180http://orcid.org/0000-0002-1835-0303http://orcid.org/0000-0002-6464-1566http://orcid.org/0000-0002-8052-5389Relata-se um caso de melanoma oral disseminado em uma cadela de dois anos, com protrusão de bulbo ocular unilateral e quadro convulsivo progressivo. Os exames de imagem revelaram aumento de volume nas regiões submandibular, maxilar e cerebral, padrão nodular pulmonar e aumento das dimensões ovarianas. A citologia da massa submandibular indicou proliferação epitelial maligna, enquanto a biópsia excisional foi sugestiva de melanoma amelanótico. Na necropsia, havia uma massa gengival localmente infiltrativa e nodulações brancas nos linfonodos, nos rins, no pulmão, no cérebro e nos ovários, indicativas de metástase. O diagnóstico histopatológico consistiu de neoplasia maligna metastática indiferenciada, indicativo de melanoma amelanótico. Células caracterizadas por núcleo com cromatina espessa, múltiplos nucléolos bem evidentes, mitoses atípicas e multinucleações consistiram nos principais critérios de malignidade. No espaço peritrabecular ósseo facial, havia rara diferenciação pigmentar melanocítica, confirmada histoquimicamente pela técnica de Fontana-Massom e Giemsa. Algumas células foram positivas pela imuno-histoquímica para PNL-2 e Melan-A, e o diagnóstico de melanoma amelanótico disseminado foi firmado. A indiferenciação neoplásica marcante, com disseminação metastática multissistêmica e acometimento mútuo de sítios anatômicos pouco comuns, conjuntamente com a ampla variação dos padrões celulares, foi responsável pelo desafio diagnóstico do presente caso, ressaltando o papel decisivo da imuno-histoquímica para confirmação diagnóstica. A importância clínica deste trabalho consiste ainda em alertar a comunidade clínica e científica acerca da dificuldade diagnóstica, devendo-se considerar o melanoma amelanótico como diferencial mesmo em casos de lesões orais menos perceptíveis e/ou desprovidas de pigmentação.A case of disseminated oral melanoma in a two year old female dog with unilateral protuberance of the eye bulb and progressive seizure is described. Imaging exams revealed increase of the submandibular, maxillary and cerebral regions, nodular pattern in lungs and increased ovarian dimensions. The cytology of the submandibular mass indicated a malignant epithelial proliferation, whereas the excisional biopsy indicated an amelanotic melanoma. At necropsy, a locally infiltrating gingival mass and white nodules in the lymph nodes, kidneys, lung, brain and ovaries were observed, indicative of metastases. Histopathological diagnosis consisted of an undifferentiated malignant metastatic neoplasm. Nucleus with coarse chromatin, prominent nucleoli, bizarre mitotic figures and multinucleated cells were the major malignant features. There was a poor melanocytic pigment differentiation in the peritrabecular space of facial bones, confirmed by Fontana-Masson and Giemsa histochemical techniques. Only a few cells were immunohistochemically positive for PNL-2 and Melan-A and the diagnosis of a disseminated amelanotic melanoma was performed. The diagnostic challenge was based on marked neoplastic undifferentiation, with multisystemic metastasis and mutual involvement of uncommon anatomic sites, associated with a large variability of cellular patterns, highlighting the decisive role of immunohistochemistry for diagnostic confirmation. Therefore, the clinical importance of this study is to warn the clinical and scientific community about the diagnostic challenge, considering the amelanotic melanoma as a differential even in cases of poorly apparent and/or nonpigmented oral lesions.porUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE PATOLOGIAVET - DEPARTAMENTO DE CLÍNICA E CIRURGIAArquivo Brasileiro de Medicina Veterinária e ZootecniaCãoNeoplasiasMelanoma amelanóticoMetástase neoplásicaOvárioCãoNeoplasiaAmelanóticoMetástaseOvárioMelanoma oral amelanótico metastático com acometimento neurológico e gonadal em um cão fêmea - relato de casoMetastatic oral amelanotic melanoma with neurologic and gonadal involvement in a female dog ˗ case reportinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/abmvz/a/pchfHgfDmdNTtsnB3vMY8VC/?lang=pt#Carlos Eduardo Bastos LopesMatheus Vilardo Lóes MoreiraBárbara Andrade de CarvalhoPablo Herthel CarvalhoEnio FerreiraRoberto Mauricio Carvalho GuedesRoselene Eccoapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGORIGINALMelanoma oral amelanótico metastático com acometimento neurológico e gonadal em um cão fêmea ˗ relato de caso.pdfMelanoma oral amelanótico metastático com acometimento neurológico e gonadal em um cão fêmea ˗ relato de caso.pdfapplication/pdf677373https://repositorio.ufmg.br/bitstream/1843/56455/2/Melanoma%20oral%20amelan%c3%b3tico%20metast%c3%a1tico%20com%20acometimento%20neurol%c3%b3gico%20e%20gonadal%20em%20um%20c%c3%a3o%20f%c3%aamea%20%cb%97%20relato%20de%20caso.pdf5d1d2857c0d1b04602773e13dc485d71MD52LICENSELicense.txtLicense.txttext/plain; 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