Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines

• Autor(a) principal: Gabriel Silva Marques Borges
• Data de Publicação: 2021
• Outros Autores: Elaine Maria de Souza Fagundes, Lucas Antônio Miranda Ferreira, Pedro Henrique Dias Moura Prazeres, Ângelo Malachias de Souza, Maria Irene Yoshida, José Mário Carneiro Vilela, Aline Teixeira Maciel e Silva, Mariana Silva Oliveira, Dawidson Assis Gomes, Margareth Spangler Andrade
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: http://dx.doi.org/10.1590/s2175-97902020000418497; http://hdl.handle.net/1843/52599; https://orcid.org/0000-0002-7360-8231; https://orcid.org/0000-0002-0539-9052; https://orcid.org/0000-0003-2474-5536; https://orcid.org/0000-0002-1933-3230; https://orcid.org/0000-0002-8703-4283; https://orcid.org/0000-0001-7714-991X; https://orcid.org/0000-0002-0412-1622
• topic: Cancer; Sclareol; Solid lipid nanoparticles; Nanostructured lipid carriers; Small-angle X-ray scattering; Câncer; Nanopartículas; Lipídios

Sclareol (SC) is arousing great interest due to its cytostatic and cytotoxic activities in several cancer cell lines. However, its hydrophobicity is a limiting factor for its in vivo administration. One way to solve this problem is through nanoencapsulation. Therefore, solid lipid nanoparticles (SLN-SC) and nanostructured lipid carriers (NLC-SC) loaded with SC were produced and compared regarding their physicochemical properties. NLC-SC showed better SC encapsulation than SLN-SC and was chosen to be compared with free SC in human cancer cell lines (MDA-MB-231 and HCT-116). Free SC had slightly higher cytotoxicity than NLC-SC and produced subdiploid DNA content in both cell lines. On the other hand, NLC-SC led to subdiploid content in MDA-MB-231 cells and G2/M checkpoint arrest in HCT-116 cells. These findings suggest that SC encapsulation in NLC is a way to allow the in vivo administration of SC and might alter its biological properties.