Antioxidant capacity is decreased in Alzheimer’s disease and mild cognitive impairment patients
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/46372 |
Resumo: | We investigated through measurements in serum, the occurrence of oxidative stress in patients with Alzheimer’s disease (AD), mild cognitive impairment (MCI) and healthy elderly controls. Possible correlations between a genetic risk factor for AD, the allele ε4 of the apolipoprotein E (APOE) gene, and oxidative stress were also investigated. Through Thiobarbituric Acid Reactive Substances (TBARS) assay serum lipid peroxidation products of AD patients, MCI patients and controls were measured. We also analyzed the participants’ serum antioxidant status through 3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye reduction. No difference between the groups was observed concerning TBARS levels (p = 0.212). Controls had a higher antioxidant status compared to AD (controls: 0.43 ± 0.061 O.D; AD: 0.39 ± 0.065 O.D), p = 0.002, and MCI patients (0.381 ± 0.065 O.D), p = 0.001. No difference concerning antioxidant status or TBARS levels was associated with the ε4 APOE allele. Oxidative stress in MCI and AD patients seems to be evidenced in serum by a reduction of the antioxidant system capacity rather than change in the TBARS levels. MTT assay for evaluation of antioxidant status could be helpful in therapeutic intervention and guide changes in the life-style of individuals in early stages of cognition impairment. |
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2022-10-19T13:47:11Z2022-10-19T13:47:11Z2019-04941781862249-9571http://hdl.handle.net/1843/46372We investigated through measurements in serum, the occurrence of oxidative stress in patients with Alzheimer’s disease (AD), mild cognitive impairment (MCI) and healthy elderly controls. Possible correlations between a genetic risk factor for AD, the allele ε4 of the apolipoprotein E (APOE) gene, and oxidative stress were also investigated. Through Thiobarbituric Acid Reactive Substances (TBARS) assay serum lipid peroxidation products of AD patients, MCI patients and controls were measured. We also analyzed the participants’ serum antioxidant status through 3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye reduction. No difference between the groups was observed concerning TBARS levels (p = 0.212). Controls had a higher antioxidant status compared to AD (controls: 0.43 ± 0.061 O.D; AD: 0.39 ± 0.065 O.D), p = 0.002, and MCI patients (0.381 ± 0.065 O.D), p = 0.001. No difference concerning antioxidant status or TBARS levels was associated with the ε4 APOE allele. Oxidative stress in MCI and AD patients seems to be evidenced in serum by a reduction of the antioxidant system capacity rather than change in the TBARS levels. MTT assay for evaluation of antioxidant status could be helpful in therapeutic intervention and guide changes in the life-style of individuals in early stages of cognition impairment.Investigamos através de medidas no soro, a ocorrência de estresse oxidativo em pacientes com Doença de Alzheimer (DA), comprometimento cognitivo leve (CCL) e controles idosos saudáveis. Possível correlações entre um fator de risco genético para DA, o alelo ε4 do gene da apolipoproteína E (APOE), e estresse oxidativo também foram investigados. Através de substâncias reativas de ácido tiobarbitúrico (TBARS) os produtos de peroxidação lipídica sérica de pacientes com DA, pacientes com MCI e controles foram medido. Também analisamos o status antioxidante sérico dos participantes por meio de 3-(4,5-Redução de corante de brometo de dimetiltiazol-2-il)-2,5-difeniltetrazólio (MTT). Nenhuma diferença entre os grupos foi observado quanto aos níveis de TBARS (p = 0,212). Os controles tiveram um antioxidante mais alto status em comparação com AD (controles: 0,43 ± 0,061 O.D; AD: 0,39 ± 0,065 O.D), p = 0,002 e MCI pacientes (0,381 ± 0,065 O.D), p = 0,001. Nenhuma diferença em relação ao status antioxidante ou níveis de TBARS foi associado ao alelo ε4 APOE. O estresse oxidativo em pacientes com CCL e DA parece ser evidenciado no soro por uma redução da capacidade do sistema antioxidante ao invés de alteração na Níveis de TBARS. O ensaio MTT para avaliação do status antioxidante pode ser útil na terapia intervenção e orientar mudanças no estilo de vida de indivíduos em estágios iniciais de comprometimento da cognição.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorengUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASMED - DEPARTAMENTO DE CLÍNICA MÉDICAInternational Journal of Health Sciences and ResearchDoença de AlzheimerBiomarcadoresEnvelhecimentoDemênciaAlzheimer’s diseaseBiochemical markersAgingDementiaMolecular markersAntioxidant capacity is decreased in Alzheimer’s disease and mild cognitive impairment patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.ijhsr.org/IJHSR_Vol.9_Issue.4_April2019/IJHSR_Abstract.026.htmlMayara Chaves FariaGisele Santos GonçalvesRita Carolina Figueiredo DuarteMaria Aparecida Camargos BicalhoLuís Felipe José Ravic de MirandaKarina Braga Gomes BorgesLirlândia Pires de SousaMaria das Graças Carvalhoapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/46372/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALAntioxidant Capacity is Decreased in Alzheimers Disease and Mild Cognitive Impairment Patients.pdfAntioxidant Capacity is Decreased in Alzheimers Disease and Mild Cognitive Impairment Patients.pdfapplication/pdf771134https://repositorio.ufmg.br/bitstream/1843/46372/2/Antioxidant%20Capacity%20is%20Decreased%20in%20Alzheimers%20Disease%20and%20Mild%20Cognitive%20Impairment%20Patients.pdfccf9107e3becef25dd7bcc77e7333722MD521843/463722022-10-19 10:47:11.334oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-10-19T13:47:11Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Antioxidant capacity is decreased in Alzheimer’s disease and mild cognitive impairment patients |
title |
Antioxidant capacity is decreased in Alzheimer’s disease and mild cognitive impairment patients |
spellingShingle |
Antioxidant capacity is decreased in Alzheimer’s disease and mild cognitive impairment patients Mayara Chaves Faria Alzheimer’s disease Biochemical markers Aging Dementia Molecular markers Doença de Alzheimer Biomarcadores Envelhecimento Demência |
title_short |
Antioxidant capacity is decreased in Alzheimer’s disease and mild cognitive impairment patients |
title_full |
Antioxidant capacity is decreased in Alzheimer’s disease and mild cognitive impairment patients |
title_fullStr |
Antioxidant capacity is decreased in Alzheimer’s disease and mild cognitive impairment patients |
title_full_unstemmed |
Antioxidant capacity is decreased in Alzheimer’s disease and mild cognitive impairment patients |
title_sort |
Antioxidant capacity is decreased in Alzheimer’s disease and mild cognitive impairment patients |
author |
Mayara Chaves Faria |
author_facet |
Mayara Chaves Faria Gisele Santos Gonçalves Rita Carolina Figueiredo Duarte Maria Aparecida Camargos Bicalho Luís Felipe José Ravic de Miranda Karina Braga Gomes Borges Lirlândia Pires de Sousa Maria das Graças Carvalho |
author_role |
author |
author2 |
Gisele Santos Gonçalves Rita Carolina Figueiredo Duarte Maria Aparecida Camargos Bicalho Luís Felipe José Ravic de Miranda Karina Braga Gomes Borges Lirlândia Pires de Sousa Maria das Graças Carvalho |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Mayara Chaves Faria Gisele Santos Gonçalves Rita Carolina Figueiredo Duarte Maria Aparecida Camargos Bicalho Luís Felipe José Ravic de Miranda Karina Braga Gomes Borges Lirlândia Pires de Sousa Maria das Graças Carvalho |
dc.subject.por.fl_str_mv |
Alzheimer’s disease Biochemical markers Aging Dementia Molecular markers |
topic |
Alzheimer’s disease Biochemical markers Aging Dementia Molecular markers Doença de Alzheimer Biomarcadores Envelhecimento Demência |
dc.subject.other.pt_BR.fl_str_mv |
Doença de Alzheimer Biomarcadores Envelhecimento Demência |
description |
We investigated through measurements in serum, the occurrence of oxidative stress in patients with Alzheimer’s disease (AD), mild cognitive impairment (MCI) and healthy elderly controls. Possible correlations between a genetic risk factor for AD, the allele ε4 of the apolipoprotein E (APOE) gene, and oxidative stress were also investigated. Through Thiobarbituric Acid Reactive Substances (TBARS) assay serum lipid peroxidation products of AD patients, MCI patients and controls were measured. We also analyzed the participants’ serum antioxidant status through 3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye reduction. No difference between the groups was observed concerning TBARS levels (p = 0.212). Controls had a higher antioxidant status compared to AD (controls: 0.43 ± 0.061 O.D; AD: 0.39 ± 0.065 O.D), p = 0.002, and MCI patients (0.381 ± 0.065 O.D), p = 0.001. No difference concerning antioxidant status or TBARS levels was associated with the ε4 APOE allele. Oxidative stress in MCI and AD patients seems to be evidenced in serum by a reduction of the antioxidant system capacity rather than change in the TBARS levels. MTT assay for evaluation of antioxidant status could be helpful in therapeutic intervention and guide changes in the life-style of individuals in early stages of cognition impairment. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-04 |
dc.date.accessioned.fl_str_mv |
2022-10-19T13:47:11Z |
dc.date.available.fl_str_mv |
2022-10-19T13:47:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/46372 |
dc.identifier.issn.pt_BR.fl_str_mv |
2249-9571 |
identifier_str_mv |
2249-9571 |
url |
http://hdl.handle.net/1843/46372 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
International Journal of Health Sciences and Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS MED - DEPARTAMENTO DE CLÍNICA MÉDICA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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Universidade Federal de Minas Gerais (UFMG) |
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UFMG |
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Repositório Institucional da UFMG |
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