Development of a low-cost cellulase production process using Trichoderma reesei for brazilian biorefineries
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1186/s13068-017-0717-0 http://hdl.handle.net/1843/48798 |
Resumo: | Background: During the past few years, the first industrial‑scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. Depending on the region, transport of cel‑ lulases from a central production facility to a biorefinery may significantly add to enzyme cost. The aim of the present study was to develop a simple, cost‑efficient cellulase production process that could be employed locally at a Brazilian sugarcane biorefinery. Results: Our work focused on two main topics: growth medium formulation and strain improvement. We evalu‑ated several Brazilian low‑cost industrial residues for their potential in cellulase production. Among the solid residues evaluated, soybean hulls were found to display clearly the most desirable characteristics. We engineered a Trichoderma reesei strain to secrete cellulase in the presence of repressing sugars, enabling the use of sugarcane molasses as an additional carbon source. In addition, we added a heterologous β‑glucosidase to improve the performance of the produced enzymes in hydrolysis. Finally, the addition of an invertase gene from Aspegillus niger into our strain allowed it to consume sucrose from sugarcane molasses directly. Preliminary cost analysis showed that the overall process can provide for very low‑cost enzyme with good hydrolysis performance on industrially pre‑treated sugarcane straw. Conclusions: In this study, we showed that with relatively few genetic modifications and the right growth medium it is possible to produce considerable amounts of well‑performing cellulase at very low cost in Brazil using T. reesei. With further enhancements and optimization, such a system could provide a viable alternative to delivered commercial cellulases. |
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2023-01-09T19:34:44Z2023-01-09T19:34:44Z201710117https://doi.org/10.1186/s13068-017-0717-02731-3654http://hdl.handle.net/1843/48798Background: During the past few years, the first industrial‑scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. Depending on the region, transport of cel‑ lulases from a central production facility to a biorefinery may significantly add to enzyme cost. The aim of the present study was to develop a simple, cost‑efficient cellulase production process that could be employed locally at a Brazilian sugarcane biorefinery. Results: Our work focused on two main topics: growth medium formulation and strain improvement. We evalu‑ated several Brazilian low‑cost industrial residues for their potential in cellulase production. Among the solid residues evaluated, soybean hulls were found to display clearly the most desirable characteristics. We engineered a Trichoderma reesei strain to secrete cellulase in the presence of repressing sugars, enabling the use of sugarcane molasses as an additional carbon source. In addition, we added a heterologous β‑glucosidase to improve the performance of the produced enzymes in hydrolysis. Finally, the addition of an invertase gene from Aspegillus niger into our strain allowed it to consume sucrose from sugarcane molasses directly. Preliminary cost analysis showed that the overall process can provide for very low‑cost enzyme with good hydrolysis performance on industrially pre‑treated sugarcane straw. Conclusions: In this study, we showed that with relatively few genetic modifications and the right growth medium it is possible to produce considerable amounts of well‑performing cellulase at very low cost in Brazil using T. reesei. With further enhancements and optimization, such a system could provide a viable alternative to delivered commercial cellulases.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASBiotechnology for Biofuels and BioproductsCelulase Cana-de-açúcarAçucar - UsinasCellulaseEnzymeTrichoderma reeseiSugarcaneDevelopment of a low-cost cellulase production process using Trichoderma reesei for brazilian biorefineriesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-017-0717-0Simo ElliläLucas FonsecaCristiane UchimaJúnio Cota SilvaGustavo Henrique GoldmanMarkku SaloheimoVera SaconMatti Siika-ahoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/48798/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALDevelopment of a low-cost cellulase_ Junio Cota.pdfDevelopment of a low-cost cellulase_ Junio Cota.pdfapplication/pdf2017092https://repositorio.ufmg.br/bitstream/1843/48798/2/Development%20of%20a%20low-cost%20cellulase_%20Junio%20Cota.pdf71cb0ac3cfa43c98ab3895602737508bMD521843/487982023-01-09 16:34:44.283oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-01-09T19:34:44Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Development of a low-cost cellulase production process using Trichoderma reesei for brazilian biorefineries |
title |
Development of a low-cost cellulase production process using Trichoderma reesei for brazilian biorefineries |
spellingShingle |
Development of a low-cost cellulase production process using Trichoderma reesei for brazilian biorefineries Simo Ellilä Cellulase Enzyme Trichoderma reesei Sugarcane Celulase Cana-de-açúcar Açucar - Usinas |
title_short |
Development of a low-cost cellulase production process using Trichoderma reesei for brazilian biorefineries |
title_full |
Development of a low-cost cellulase production process using Trichoderma reesei for brazilian biorefineries |
title_fullStr |
Development of a low-cost cellulase production process using Trichoderma reesei for brazilian biorefineries |
title_full_unstemmed |
Development of a low-cost cellulase production process using Trichoderma reesei for brazilian biorefineries |
title_sort |
Development of a low-cost cellulase production process using Trichoderma reesei for brazilian biorefineries |
author |
Simo Ellilä |
author_facet |
Simo Ellilä Lucas Fonseca Cristiane Uchima Júnio Cota Silva Gustavo Henrique Goldman Markku Saloheimo Vera Sacon Matti Siika-aho |
author_role |
author |
author2 |
Lucas Fonseca Cristiane Uchima Júnio Cota Silva Gustavo Henrique Goldman Markku Saloheimo Vera Sacon Matti Siika-aho |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Simo Ellilä Lucas Fonseca Cristiane Uchima Júnio Cota Silva Gustavo Henrique Goldman Markku Saloheimo Vera Sacon Matti Siika-aho |
dc.subject.por.fl_str_mv |
Cellulase Enzyme Trichoderma reesei Sugarcane |
topic |
Cellulase Enzyme Trichoderma reesei Sugarcane Celulase Cana-de-açúcar Açucar - Usinas |
dc.subject.other.pt_BR.fl_str_mv |
Celulase Cana-de-açúcar Açucar - Usinas |
description |
Background: During the past few years, the first industrial‑scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. Depending on the region, transport of cel‑ lulases from a central production facility to a biorefinery may significantly add to enzyme cost. The aim of the present study was to develop a simple, cost‑efficient cellulase production process that could be employed locally at a Brazilian sugarcane biorefinery. Results: Our work focused on two main topics: growth medium formulation and strain improvement. We evalu‑ated several Brazilian low‑cost industrial residues for their potential in cellulase production. Among the solid residues evaluated, soybean hulls were found to display clearly the most desirable characteristics. We engineered a Trichoderma reesei strain to secrete cellulase in the presence of repressing sugars, enabling the use of sugarcane molasses as an additional carbon source. In addition, we added a heterologous β‑glucosidase to improve the performance of the produced enzymes in hydrolysis. Finally, the addition of an invertase gene from Aspegillus niger into our strain allowed it to consume sucrose from sugarcane molasses directly. Preliminary cost analysis showed that the overall process can provide for very low‑cost enzyme with good hydrolysis performance on industrially pre‑treated sugarcane straw. Conclusions: In this study, we showed that with relatively few genetic modifications and the right growth medium it is possible to produce considerable amounts of well‑performing cellulase at very low cost in Brazil using T. reesei. With further enhancements and optimization, such a system could provide a viable alternative to delivered commercial cellulases. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2023-01-09T19:34:44Z |
dc.date.available.fl_str_mv |
2023-01-09T19:34:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/48798 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1186/s13068-017-0717-0 |
dc.identifier.issn.pt_BR.fl_str_mv |
2731-3654 |
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https://doi.org/10.1186/s13068-017-0717-0 http://hdl.handle.net/1843/48798 |
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2731-3654 |
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eng |
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eng |
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Biotechnology for Biofuels and Bioproducts |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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