Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse

Detalhes bibliográficos
Autor(a) principal: Priscila Fantini
Data de Publicação: 2022
Outros Autores: Román Jimenez-Aristizabal, Antoni Iborra Obiols, Ana Luísa Soares de Miranda, Renata de Pino Albuquerque Maranhão, Fabíola de Oliveira Paes Leme, Maristela Silveira Palhares, Marta Prades
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1590/1678-4162-12257
http://hdl.handle.net/1843/67586
http://orcid.org/0000-0002-2047-6626
http://orcid.org/0000-0002-7833-4786
http://orcid.org/0000-0002-8984-5151
http://orcid.org/0000-0003-4837-9374
http://orcid.org/0000-0002-2084-672X
http://orcid.org/0000-0002-4013-0147
http://orcid.org/0000-0003-2606-4320
http://orcid.org/0000-0003-2713-9232
Resumo: Platelet-rich plasma (PRP) has been proposed as an agent to accelerate the healing process and stimulate the regenerative capacity of tissues due to its abundance of growth factors. A large variety of kits and protocols are available to obtain PRP by different cell-separation systems. However, the lack of standardization may lead to inconsistent results. The aim of this study was to characterize cellular composition, platelet parameters using the ADVIA 120 flow cytometer, and TGF-β1 concentration from the PRP product obtained through a closed system, using simple centrifugation. Six clinically healthy horses were used in this study. The protocol in the closed system resulted in approximately 1.6-fold higher platelet and approximately 2.0-fold lower white blood cell concentrations in comparison with whole blood values. The evaluated system was efficient in concentrating platelets and in retrieving a small number of leukocytes, using a protocol of single centrifugation at low speed.
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spelling 2024-04-23T14:40:47Z2024-04-23T14:40:47Z202274193100https://doi.org/10.1590/1678-4162-122571678-4162http://hdl.handle.net/1843/67586http://orcid.org/0000-0002-2047-6626http://orcid.org/0000-0002-7833-4786http://orcid.org/0000-0002-8984-5151http://orcid.org/0000-0003-4837-9374http://orcid.org/0000-0002-2084-672Xhttp://orcid.org/0000-0002-4013-0147http://orcid.org/0000-0003-2606-4320http://orcid.org/0000-0003-2713-9232Platelet-rich plasma (PRP) has been proposed as an agent to accelerate the healing process and stimulate the regenerative capacity of tissues due to its abundance of growth factors. A large variety of kits and protocols are available to obtain PRP by different cell-separation systems. However, the lack of standardization may lead to inconsistent results. The aim of this study was to characterize cellular composition, platelet parameters using the ADVIA 120 flow cytometer, and TGF-β1 concentration from the PRP product obtained through a closed system, using simple centrifugation. Six clinically healthy horses were used in this study. The protocol in the closed system resulted in approximately 1.6-fold higher platelet and approximately 2.0-fold lower white blood cell concentrations in comparison with whole blood values. The evaluated system was efficient in concentrating platelets and in retrieving a small number of leukocytes, using a protocol of single centrifugation at low speed.O plasma rico em plaquetas (PRP) tem sido proposto como um agente para acelerar o processo de cicatrização e estimular a capacidade regenerativa dos tecidos, devido à sua abundância de fatores de crescimento. Uma grande variedade de kits e de protocolos está disponível para obtenção de PRP, utilizando-se diferentes sistemas de separação de células. No entanto, a falta de padronização pode levar a resultados inconsistentes. O objetivo deste estudo foi caracterizar a composição celular, os parâmetros plaquetários pelo citômetro de fluxo ADVIA 120 e a concentração de TGF-β1 do PRP obtido em sistema fechado, por centrifugação simples. Seis cavalos clinicamente saudáveis ​​foram usados ​​neste estudo. O protocolo no sistema fechado resultou em concentrações de plaquetas aproximadamente 1,6 vez maior e concentrações de leucócitos aproximadamente 2,0 vezes menores em comparação com os valores do sangue total. O sistema avaliado foi eficiente na concentração de plaquetas e na recuperação de um pequeno número de leucócitos, utilizando um protocolo de centrifugação única em baixa velocidade.engUniversidade Federal de Minas GeraisUFMGBrasilVET - DEPARTAMENTO DE CLÍNICA E CIRURGIAArquivo Brasileiro de Medicina Veterinária e ZootecniaPlasma rico em plaquetasEstruturas celularesCentrifugaçãoEquinosPRPGrowth factorsPlatelet concentrateConcentration protocolCellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horseComponentes celulares e TGF-β1 do plasma rico em plaquetas obtido por sistema fechado em equinosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/abmvz/a/dPZcm5L4mft7hXN6kKBzH6Q/?lang=en#Priscila FantiniRomán Jimenez-AristizabalAntoni Iborra ObiolsAna Luísa Soares de MirandaRenata de Pino Albuquerque MaranhãoFabíola de Oliveira Paes LemeMaristela Silveira PalharesMarta Pradesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/67586/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALCellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse.pdfCellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse.pdfapplication/pdf250045https://repositorio.ufmg.br/bitstream/1843/67586/2/Cellular%20components%20and%20TGF-%ce%b21%20content%20of%20a%20closed%20Tube%20system%20for%20Platelet%20Rich%20Plasma%20acquisition%20in%20horse.pdf8768ece358ab0b13f1cf078cbb2737daMD521843/675862024-04-23 11:40:48.38oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2024-04-23T14:40:48Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse
dc.title.alternative.pt_BR.fl_str_mv Componentes celulares e TGF-β1 do plasma rico em plaquetas obtido por sistema fechado em equinos
title Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse
spellingShingle Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse
Priscila Fantini
PRP
Growth factors
Platelet concentrate
Concentration protocol
Plasma rico em plaquetas
Estruturas celulares
Centrifugação
Equinos
title_short Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse
title_full Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse
title_fullStr Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse
title_full_unstemmed Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse
title_sort Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse
author Priscila Fantini
author_facet Priscila Fantini
Román Jimenez-Aristizabal
Antoni Iborra Obiols
Ana Luísa Soares de Miranda
Renata de Pino Albuquerque Maranhão
Fabíola de Oliveira Paes Leme
Maristela Silveira Palhares
Marta Prades
author_role author
author2 Román Jimenez-Aristizabal
Antoni Iborra Obiols
Ana Luísa Soares de Miranda
Renata de Pino Albuquerque Maranhão
Fabíola de Oliveira Paes Leme
Maristela Silveira Palhares
Marta Prades
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Priscila Fantini
Román Jimenez-Aristizabal
Antoni Iborra Obiols
Ana Luísa Soares de Miranda
Renata de Pino Albuquerque Maranhão
Fabíola de Oliveira Paes Leme
Maristela Silveira Palhares
Marta Prades
dc.subject.por.fl_str_mv PRP
Growth factors
Platelet concentrate
Concentration protocol
topic PRP
Growth factors
Platelet concentrate
Concentration protocol
Plasma rico em plaquetas
Estruturas celulares
Centrifugação
Equinos
dc.subject.other.pt_BR.fl_str_mv Plasma rico em plaquetas
Estruturas celulares
Centrifugação
Equinos
description Platelet-rich plasma (PRP) has been proposed as an agent to accelerate the healing process and stimulate the regenerative capacity of tissues due to its abundance of growth factors. A large variety of kits and protocols are available to obtain PRP by different cell-separation systems. However, the lack of standardization may lead to inconsistent results. The aim of this study was to characterize cellular composition, platelet parameters using the ADVIA 120 flow cytometer, and TGF-β1 concentration from the PRP product obtained through a closed system, using simple centrifugation. Six clinically healthy horses were used in this study. The protocol in the closed system resulted in approximately 1.6-fold higher platelet and approximately 2.0-fold lower white blood cell concentrations in comparison with whole blood values. The evaluated system was efficient in concentrating platelets and in retrieving a small number of leukocytes, using a protocol of single centrifugation at low speed.
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2024-04-23T14:40:47Z
dc.date.available.fl_str_mv 2024-04-23T14:40:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/67586
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1590/1678-4162-12257
dc.identifier.issn.pt_BR.fl_str_mv 1678-4162
dc.identifier.orcid.pt_BR.fl_str_mv http://orcid.org/0000-0002-2047-6626
http://orcid.org/0000-0002-7833-4786
http://orcid.org/0000-0002-8984-5151
http://orcid.org/0000-0003-4837-9374
http://orcid.org/0000-0002-2084-672X
http://orcid.org/0000-0002-4013-0147
http://orcid.org/0000-0003-2606-4320
http://orcid.org/0000-0003-2713-9232
url https://doi.org/10.1590/1678-4162-12257
http://hdl.handle.net/1843/67586
http://orcid.org/0000-0002-2047-6626
http://orcid.org/0000-0002-7833-4786
http://orcid.org/0000-0002-8984-5151
http://orcid.org/0000-0003-4837-9374
http://orcid.org/0000-0002-2084-672X
http://orcid.org/0000-0002-4013-0147
http://orcid.org/0000-0003-2606-4320
http://orcid.org/0000-0003-2713-9232
identifier_str_mv 1678-4162
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
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