The β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruzi
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/44333 |
Resumo: | BACKGROUND The infection led by Trypanosoma cruzi persists in mammalian tissues causing an inflammatory imbalance. Carvedilol (Cv), a non-selective beta blocker drug indicated to treat heart failure and antihypertensive has shown to promote antioxidant and immunomodulatory properties which might improve the inflammation induced by T. cruzi. OBJECTIVES Evaluate the role of Cv on the inflammatory response of C57BL/6 mice acutely infected with the Colombian strain of T. cruzi. METHODS Animals were infected with the Colombian strain of T. cruzi and treated with Cv (25 mg/kg/day), benznidazole (Bz) (100 mg/kg/day) or their combination. On the 28th day of infection and 23 days of treatment, the euthanasia occurred, and the heart preserved for histopathological, oxidative stress (SOD, catalase, TBARs, carbonylated proteins) and plasma (CCL2, CCL5, TNF, IL-10) analyses. Parasitaemia and survival were assessed along the infection. FINDINGS Cv decreased TBARs, but increased the mortality rate, the parasitaemia and the levels of CCL2, CCL5, catalase and the inflammatory infiltrate in the cardiac tissue. Bz led the reduction of the inflammatory infiltrate and circulating levels of oxidative stress and inflammatory mediators in the infected mice. MAIN CONCLUSIONS Our data suggest that Cv, in this experimental model using the Colombian strain of T. cruzi, caused damage to the host. |
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2022-08-17T20:00:56Z2022-08-17T20:00:56Z20181131110.1590/0074-027601802711678-8060http://hdl.handle.net/1843/44333BACKGROUND The infection led by Trypanosoma cruzi persists in mammalian tissues causing an inflammatory imbalance. Carvedilol (Cv), a non-selective beta blocker drug indicated to treat heart failure and antihypertensive has shown to promote antioxidant and immunomodulatory properties which might improve the inflammation induced by T. cruzi. OBJECTIVES Evaluate the role of Cv on the inflammatory response of C57BL/6 mice acutely infected with the Colombian strain of T. cruzi. METHODS Animals were infected with the Colombian strain of T. cruzi and treated with Cv (25 mg/kg/day), benznidazole (Bz) (100 mg/kg/day) or their combination. On the 28th day of infection and 23 days of treatment, the euthanasia occurred, and the heart preserved for histopathological, oxidative stress (SOD, catalase, TBARs, carbonylated proteins) and plasma (CCL2, CCL5, TNF, IL-10) analyses. Parasitaemia and survival were assessed along the infection. FINDINGS Cv decreased TBARs, but increased the mortality rate, the parasitaemia and the levels of CCL2, CCL5, catalase and the inflammatory infiltrate in the cardiac tissue. Bz led the reduction of the inflammatory infiltrate and circulating levels of oxidative stress and inflammatory mediators in the infected mice. MAIN CONCLUSIONS Our data suggest that Cv, in this experimental model using the Colombian strain of T. cruzi, caused damage to the host.ANTECEDENTES A infecção pelo Trypanosoma cruzi persiste em tecidos de mamíferos causando um desequilíbrio inflamatório. O carvedilol (Cv), um betabloqueador não seletivo indicado para o tratamento da insuficiência cardíaca e anti-hipertensivo, demonstrou promover propriedades antioxidantes e imunomoduladoras que podem melhorar a inflamação induzida pelo T. cruzi. OBJETIVOS Avaliar o papel da Cv na resposta inflamatória de camundongos C57BL/6 agudamente infectados com a cepa colombiana de T. cruzi. MÉTODOS Os animais foram infectados com a cepa colombiana de T. cruzi e tratados com Cv (25 mg/kg/dia), benznidazol (Bz) (100 mg/kg/dia) ou sua combinação. No 28º dia de infecção e 23 dias de tratamento, ocorreu a eutanásia e o coração foi preservado para análises histopatológicas, de estresse oxidativo (SOD, catalase, TBARs, proteínas carboniladas) e plasmáticas (CCL2, CCL5, TNF, IL-10). Parasitemia e sobrevivência foram avaliadas ao longo da infecção. DADOS Cv diminuiu TBARs, mas aumentou a taxa de mortalidade, a parasitemia e os níveis de CCL2, CCL5, catalase e o infiltrado inflamatório no tecido cardíaco. Bz liderou a redução do infiltrado inflamatório e dos níveis circulantes de estresse oxidativo e mediadores inflamatórios nos camundongos infectados. PRINCIPAIS CONCLUSÕES Nossos dados sugerem que Cv, neste modelo experimental utilizando a cepa colombiana de T. cruzi, causou danos ao hospedeiro.engUniversidade Federal de Minas GeraisUFMGBrasilICB - INSTITUTO DE CIÊNCIAS BIOLOGICASMemórias do instituto Oswaldo CruzTrypanosoma cruziInflamaçãoDoença CardíacaQuimiocinasCarvedilolThe β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruziO β-bloqueador carvedilol e o benznidazol modulam a resposta imune cardíaca na infecção aguda induzida por cepa colombiana do Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/mioc/a/vXnDtNmtgMryLnfwt4jF3ZP/abstract/?lang=enAline Luciano HortaAndré TalvaniVivian Paulino FigueiredoAna Luisa Junqueira LeiteGuilherme de Paula CostaAna Paula de Jesus MenezesCamila de Oliveira RamosTamiles Caroline Fernandes PedrosaFrank Silva BezerraPaula Melo de Abreu Vieiraapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
The β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruzi |
dc.title.alternative.pt_BR.fl_str_mv |
O β-bloqueador carvedilol e o benznidazol modulam a resposta imune cardíaca na infecção aguda induzida por cepa colombiana do Trypanosoma cruzi |
title |
The β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruzi |
spellingShingle |
The β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruzi Aline Luciano Horta Trypanosoma cruzi Inflamação Doença Cardíaca Quimiocinas Carvedilol |
title_short |
The β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruzi |
title_full |
The β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruzi |
title_fullStr |
The β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruzi |
title_full_unstemmed |
The β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruzi |
title_sort |
The β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruzi |
author |
Aline Luciano Horta |
author_facet |
Aline Luciano Horta André Talvani Vivian Paulino Figueiredo Ana Luisa Junqueira Leite Guilherme de Paula Costa Ana Paula de Jesus Menezes Camila de Oliveira Ramos Tamiles Caroline Fernandes Pedrosa Frank Silva Bezerra Paula Melo de Abreu Vieira |
author_role |
author |
author2 |
André Talvani Vivian Paulino Figueiredo Ana Luisa Junqueira Leite Guilherme de Paula Costa Ana Paula de Jesus Menezes Camila de Oliveira Ramos Tamiles Caroline Fernandes Pedrosa Frank Silva Bezerra Paula Melo de Abreu Vieira |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Aline Luciano Horta André Talvani Vivian Paulino Figueiredo Ana Luisa Junqueira Leite Guilherme de Paula Costa Ana Paula de Jesus Menezes Camila de Oliveira Ramos Tamiles Caroline Fernandes Pedrosa Frank Silva Bezerra Paula Melo de Abreu Vieira |
dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi Inflamação Doença Cardíaca Quimiocinas Carvedilol |
topic |
Trypanosoma cruzi Inflamação Doença Cardíaca Quimiocinas Carvedilol |
description |
BACKGROUND The infection led by Trypanosoma cruzi persists in mammalian tissues causing an inflammatory imbalance. Carvedilol (Cv), a non-selective beta blocker drug indicated to treat heart failure and antihypertensive has shown to promote antioxidant and immunomodulatory properties which might improve the inflammation induced by T. cruzi. OBJECTIVES Evaluate the role of Cv on the inflammatory response of C57BL/6 mice acutely infected with the Colombian strain of T. cruzi. METHODS Animals were infected with the Colombian strain of T. cruzi and treated with Cv (25 mg/kg/day), benznidazole (Bz) (100 mg/kg/day) or their combination. On the 28th day of infection and 23 days of treatment, the euthanasia occurred, and the heart preserved for histopathological, oxidative stress (SOD, catalase, TBARs, carbonylated proteins) and plasma (CCL2, CCL5, TNF, IL-10) analyses. Parasitaemia and survival were assessed along the infection. FINDINGS Cv decreased TBARs, but increased the mortality rate, the parasitaemia and the levels of CCL2, CCL5, catalase and the inflammatory infiltrate in the cardiac tissue. Bz led the reduction of the inflammatory infiltrate and circulating levels of oxidative stress and inflammatory mediators in the infected mice. MAIN CONCLUSIONS Our data suggest that Cv, in this experimental model using the Colombian strain of T. cruzi, caused damage to the host. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2022-08-17T20:00:56Z |
dc.date.available.fl_str_mv |
2022-08-17T20:00:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/44333 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1590/0074-02760180271 |
dc.identifier.issn.pt_BR.fl_str_mv |
1678-8060 |
identifier_str_mv |
10.1590/0074-02760180271 1678-8060 |
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http://hdl.handle.net/1843/44333 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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Memórias do instituto Oswaldo Cruz |
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openAccess |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS |
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Universidade Federal de Minas Gerais |
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