Animal model of arthritis and myositis induced by the Mayaro virus
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1371/journal.pntd.0007375 http://hdl.handle.net/1843/56317 https://orcid.org/0000-0002-7102-673X https://orcid.org/0000-0002-0375-8058 http://orcid.org/0000-0003-1632-2058 http://orcid.org/0000-0003-4327-9454 http://orcid.org/0000-0002-3789-5907 https://orcid.org/0000-0002-0175-642X https://orcid.org/0000-0003-3432-0769 |
Resumo: | Background The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mechanisms associated with MAYV-induced arthritis is of great importance due to the potential for its emergence, urbanization and dispersion to other regions. Methods 15-day old Balb/c mice were infected by two distinct pathways, below the forelimb and in the rear footpad. Animals were observed for a period of 21 days. During this time, they were monitored every 24 hours for disease signs, such as weight loss and muscle weakness. Histological damage in the muscles and joints was evaluated 3, 7, 10, 15 and 20 days post-infection. The cytokine profile in serum and muscles during MAYV infection was evaluated by flow cytometry at different post-infection times. For pain analysis, the animals were submitted to the von Frey test and titre in different organs was evaluated throughout the study to obtain viral kinetics. Findings Infection by two distinct pathways, below the forelimb and in the rear footpad, resulted in a homogeneous viral spread and the development of acute disease in animals. Clinical signs were observed such as ruffled fur, hunched posture, eye irritation and slight gait alteration. In the physical test, both groups presented loss of resistance, which was associated with histopathological damage, including myositis, arthritis, tenosynovitis and periostitis. The immune response was characterized by a strong inflammatory response mediated by the cytokines TNF-α, IL-6 and INF-γ and chemokine MCP-1, followed by the action of IL-10 and IL-4 cytokines. Interpretation The results showed that Balb/c mice represent a promising model to study mechanisms involved in MAYV pathogenesis and for future antiviral testing. |
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2023-07-14T22:16:35Z2023-07-14T22:16:35Z2019135120https://doi.org/10.1371/journal.pntd.00073751935-2735http://hdl.handle.net/1843/56317https://orcid.org/0000-0002-7102-673Xhttps://orcid.org/0000-0002-0375-8058http://orcid.org/0000-0003-1632-2058http://orcid.org/0000-0003-4327-9454http://orcid.org/0000-0002-3789-5907https://orcid.org/0000-0002-0175-642Xhttps://orcid.org/0000-0003-3432-0769Background The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mechanisms associated with MAYV-induced arthritis is of great importance due to the potential for its emergence, urbanization and dispersion to other regions. Methods 15-day old Balb/c mice were infected by two distinct pathways, below the forelimb and in the rear footpad. Animals were observed for a period of 21 days. During this time, they were monitored every 24 hours for disease signs, such as weight loss and muscle weakness. Histological damage in the muscles and joints was evaluated 3, 7, 10, 15 and 20 days post-infection. The cytokine profile in serum and muscles during MAYV infection was evaluated by flow cytometry at different post-infection times. For pain analysis, the animals were submitted to the von Frey test and titre in different organs was evaluated throughout the study to obtain viral kinetics. Findings Infection by two distinct pathways, below the forelimb and in the rear footpad, resulted in a homogeneous viral spread and the development of acute disease in animals. Clinical signs were observed such as ruffled fur, hunched posture, eye irritation and slight gait alteration. In the physical test, both groups presented loss of resistance, which was associated with histopathological damage, including myositis, arthritis, tenosynovitis and periostitis. The immune response was characterized by a strong inflammatory response mediated by the cytokines TNF-α, IL-6 and INF-γ and chemokine MCP-1, followed by the action of IL-10 and IL-4 cytokines. Interpretation The results showed that Balb/c mice represent a promising model to study mechanisms involved in MAYV pathogenesis and for future antiviral testing.porUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE MICROBIOLOGIAICB - DEPARTAMENTO DE MORFOLOGIAPLOS Neglected Tropical DiseasesAmérica do SulInfecçãoMayaro virus (MAYV)South AmericanClinical manifestationsInfectionAnimal model of arthritis and myositis induced by the Mayaro virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007375Franciele Martins SantosCyntia Canedo SilvaSergio Oliveira de PaulaRoberto Souza DiasMichelle Dias OliveiraIsabella Cristina Toledo Alves CostaLuciana Souza FernandesCarine Ribeiro PessoaSérgio Luís Pinto da MattaVivian Vasconcelos CostaDanielle da Glória de Souzainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56317/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALAnimal model of arthritis and myositis induced by the Mayaro virus.pdfAnimal model of arthritis and myositis induced by the Mayaro virus.pdfapplication/pdf28791954https://repositorio.ufmg.br/bitstream/1843/56317/2/Animal%20model%20of%20arthritis%20and%20myositis%20induced%20by%20the%20Mayaro%20virus.pdf6e8fb0d234b4bdc8a680a5dc6a9222e4MD521843/563172023-07-14 19:16:36.075oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-14T22:16:36Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Animal model of arthritis and myositis induced by the Mayaro virus |
title |
Animal model of arthritis and myositis induced by the Mayaro virus |
spellingShingle |
Animal model of arthritis and myositis induced by the Mayaro virus Franciele Martins Santos Mayaro virus (MAYV) South American Clinical manifestations Infection América do Sul Infecção |
title_short |
Animal model of arthritis and myositis induced by the Mayaro virus |
title_full |
Animal model of arthritis and myositis induced by the Mayaro virus |
title_fullStr |
Animal model of arthritis and myositis induced by the Mayaro virus |
title_full_unstemmed |
Animal model of arthritis and myositis induced by the Mayaro virus |
title_sort |
Animal model of arthritis and myositis induced by the Mayaro virus |
author |
Franciele Martins Santos |
author_facet |
Franciele Martins Santos Cyntia Canedo Silva Sergio Oliveira de Paula Roberto Souza Dias Michelle Dias Oliveira Isabella Cristina Toledo Alves Costa Luciana Souza Fernandes Carine Ribeiro Pessoa Sérgio Luís Pinto da Matta Vivian Vasconcelos Costa Danielle da Glória de Souza |
author_role |
author |
author2 |
Cyntia Canedo Silva Sergio Oliveira de Paula Roberto Souza Dias Michelle Dias Oliveira Isabella Cristina Toledo Alves Costa Luciana Souza Fernandes Carine Ribeiro Pessoa Sérgio Luís Pinto da Matta Vivian Vasconcelos Costa Danielle da Glória de Souza |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Franciele Martins Santos Cyntia Canedo Silva Sergio Oliveira de Paula Roberto Souza Dias Michelle Dias Oliveira Isabella Cristina Toledo Alves Costa Luciana Souza Fernandes Carine Ribeiro Pessoa Sérgio Luís Pinto da Matta Vivian Vasconcelos Costa Danielle da Glória de Souza |
dc.subject.por.fl_str_mv |
Mayaro virus (MAYV) South American Clinical manifestations Infection |
topic |
Mayaro virus (MAYV) South American Clinical manifestations Infection América do Sul Infecção |
dc.subject.other.pt_BR.fl_str_mv |
América do Sul Infecção |
description |
Background The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mechanisms associated with MAYV-induced arthritis is of great importance due to the potential for its emergence, urbanization and dispersion to other regions. Methods 15-day old Balb/c mice were infected by two distinct pathways, below the forelimb and in the rear footpad. Animals were observed for a period of 21 days. During this time, they were monitored every 24 hours for disease signs, such as weight loss and muscle weakness. Histological damage in the muscles and joints was evaluated 3, 7, 10, 15 and 20 days post-infection. The cytokine profile in serum and muscles during MAYV infection was evaluated by flow cytometry at different post-infection times. For pain analysis, the animals were submitted to the von Frey test and titre in different organs was evaluated throughout the study to obtain viral kinetics. Findings Infection by two distinct pathways, below the forelimb and in the rear footpad, resulted in a homogeneous viral spread and the development of acute disease in animals. Clinical signs were observed such as ruffled fur, hunched posture, eye irritation and slight gait alteration. In the physical test, both groups presented loss of resistance, which was associated with histopathological damage, including myositis, arthritis, tenosynovitis and periostitis. The immune response was characterized by a strong inflammatory response mediated by the cytokines TNF-α, IL-6 and INF-γ and chemokine MCP-1, followed by the action of IL-10 and IL-4 cytokines. Interpretation The results showed that Balb/c mice represent a promising model to study mechanisms involved in MAYV pathogenesis and for future antiviral testing. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2023-07-14T22:16:35Z |
dc.date.available.fl_str_mv |
2023-07-14T22:16:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/56317 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1371/journal.pntd.0007375 |
dc.identifier.issn.pt_BR.fl_str_mv |
1935-2735 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-7102-673X https://orcid.org/0000-0002-0375-8058 http://orcid.org/0000-0003-1632-2058 http://orcid.org/0000-0003-4327-9454 http://orcid.org/0000-0002-3789-5907 https://orcid.org/0000-0002-0175-642X https://orcid.org/0000-0003-3432-0769 |
url |
https://doi.org/10.1371/journal.pntd.0007375 http://hdl.handle.net/1843/56317 https://orcid.org/0000-0002-7102-673X https://orcid.org/0000-0002-0375-8058 http://orcid.org/0000-0003-1632-2058 http://orcid.org/0000-0003-4327-9454 http://orcid.org/0000-0002-3789-5907 https://orcid.org/0000-0002-0175-642X https://orcid.org/0000-0003-3432-0769 |
identifier_str_mv |
1935-2735 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
PLOS Neglected Tropical Diseases |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - DEPARTAMENTO DE MICROBIOLOGIA ICB - DEPARTAMENTO DE MORFOLOGIA |
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Universidade Federal de Minas Gerais |
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