Radiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCC
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | http://hdl.handle.net/1843/44433 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2022-08-22T13:24:41Z2022-08-22T13:24:41Z2018-11-082643344210.1007/s12253-018-0517-21532-2807http://hdl.handle.net/1843/44433CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaRadiation Therapy (RT) is a treatment option for a large number of neoplasias. However, the effect of RT on the level of hypoxia markers is poorly understood. The present study aimed to investigate the effect of RT on the levels of hypoxic markers in Oral squamous cell carcinoma (OSCC). Evaluation of HIF-1α and miR-210 levels in OSCC was performed. Then a proteomic analysis was performed to identify candidate hypoxic targets of RT. To validate proteomic studies, the effect of RT on HIF-1α, miR-210, PDH-A and LDH-A levels under hypoxia was assessed by qRT-PCR. The impact of RT in hypoxia markers was evaluated in patients to confirm in vitro results. An increase in the HIF-1α levels was observed in OSCC. RT reduced OSCC cell proliferation and migration. Interestingly, hypoxia could revert the effect of radiation on OSCC phenotype. However, proteomics analyses suggested that LDH is one of the critical targets of RT even in hypoxia. Moreover, RT decreased HIF-1α, miR-210, and LDH even in hypoxia. The current study demonstrated that hypoxia could revert the effects of RT in the OSCC context. However, RT reduces the levels HIF-1α, miR-210 and LDH in vivo and in vitro. The consequences of RT in blood should be carefully investigated.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASPathology and Oncology ResearchCâncer - RadioterapiaLactato desidrogenaseCarcinoma de células escamosasRadiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCCinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://link.springer.com/article/10.1007/s12253-018-0517-2Marcela Gonçalves de SouzaSabrina Ferreira de JesusEloá Mangabeira SantosEmisael Stênio Batista GomesArlen de Paulo Santiago FilhoEliane Macedo Sobrinho SantosLuiz Henrique da SilveiraSergio Henrique Sousa SantosAlfredo Maurício Batista de PaulaLucyana Conceição FariasAndré Luiz Sena Guimarãesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/44433/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALRadiation Therapy Reduced Blood Levels of LDH.pdfRadiation Therapy Reduced Blood Levels of LDH.pdfapplication/pdf2337374https://repositorio.ufmg.br/bitstream/1843/44433/2/Radiation%20Therapy%20Reduced%20Blood%20Levels%20of%20LDH.pdfa8f06f8382a8ba3632f93dbb5e43709eMD521843/444332022-08-22 10:24:41.963oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-08-22T13:24:41Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Radiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCC |
| title |
Radiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCC |
| spellingShingle |
Radiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCC Marcela Gonçalves de Souza Câncer - Radioterapia Lactato desidrogenase Carcinoma de células escamosas |
| title_short |
Radiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCC |
| title_full |
Radiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCC |
| title_fullStr |
Radiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCC |
| title_full_unstemmed |
Radiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCC |
| title_sort |
Radiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCC |
| author |
Marcela Gonçalves de Souza |
| author_facet |
Marcela Gonçalves de Souza Sabrina Ferreira de Jesus Eloá Mangabeira Santos Emisael Stênio Batista Gomes Arlen de Paulo Santiago Filho Eliane Macedo Sobrinho Santos Luiz Henrique da Silveira Sergio Henrique Sousa Santos Alfredo Maurício Batista de Paula Lucyana Conceição Farias André Luiz Sena Guimarães |
| author_role |
author |
| author2 |
Sabrina Ferreira de Jesus Eloá Mangabeira Santos Emisael Stênio Batista Gomes Arlen de Paulo Santiago Filho Eliane Macedo Sobrinho Santos Luiz Henrique da Silveira Sergio Henrique Sousa Santos Alfredo Maurício Batista de Paula Lucyana Conceição Farias André Luiz Sena Guimarães |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Marcela Gonçalves de Souza Sabrina Ferreira de Jesus Eloá Mangabeira Santos Emisael Stênio Batista Gomes Arlen de Paulo Santiago Filho Eliane Macedo Sobrinho Santos Luiz Henrique da Silveira Sergio Henrique Sousa Santos Alfredo Maurício Batista de Paula Lucyana Conceição Farias André Luiz Sena Guimarães |
| dc.subject.other.pt_BR.fl_str_mv |
Câncer - Radioterapia Lactato desidrogenase Carcinoma de células escamosas |
| topic |
Câncer - Radioterapia Lactato desidrogenase Carcinoma de células escamosas |
| description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| publishDate |
2018 |
| dc.date.issued.fl_str_mv |
2018-11-08 |
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2022-08-22T13:24:41Z |
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2022-08-22T13:24:41Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://hdl.handle.net/1843/44433 |
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10.1007/s12253-018-0517-2 |
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1532-2807 |
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Pathology and Oncology Research |
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Universidade Federal de Minas Gerais |
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Brasil |
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ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
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Universidade Federal de Minas Gerais |
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