Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.29374/2527-2179.bjvm004521 http://hdl.handle.net/1843/67539 http://orcid.org/0000-0003-2473-1580 http://orcid.org/0000-0002-1253-2720 https://orcid.org/0000-0002-7523-7868 http://orcid.org/0000-0002-2084-672X http://orcid.org/0000-0002-1983-3104 http://orcid.org/0000-0002-4013-0147 |
Resumo: | To determine the effect of the inclusion method on the histomorphometric evaluation of the gastrointestinal mucosa of horses, jejunum samples were collected using flank laparotomy. Sixteen mixed breed healthy adult horses, including four males and 12 females, aged 4–14 years with an average body weight of 248.40 ± 2.28 kg, were used. Jejunal biopsies were collected and analyzed by light microscopy using two methods: group 1 comprised biopsies fixed using 10% neutral formalin and embedded in paraffin; biopsies in group 2 were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.2, followed by inclusion in glycol methacrylate (GMA)-based plastic resin. Intestinal villi height, crypt depth, glandular mucosa thickness, total mucosal thickness, and villus/crypt ratio were then evaluated. For all the variables studied, with exception of the villus/crypt ratio, a significant difference (p < 0.05) was found between samples in groups 1 and 2. Processing samples for embedding in plastic resin was quicker and easier to perform compared to that for paraffin embedding. In addition, the epithelial lining of tissues in group 2 showed better resolution for conducting cytological studies under a light microscope. The difference between the studied variables can be attributed to tissue retraction caused by conventional processing for inclusion in paraffin. Therefore, the method of inclusion in GMA described in the present study appears to be a more reliable choice for morphometric evaluation of the intestinal mucosa of horses. |
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2024-04-22T20:57:22Z2024-04-22T20:57:22Z202244https://doi.org/10.29374/2527-2179.bjvm0045212527-2179http://hdl.handle.net/1843/67539http://orcid.org/0000-0003-2473-1580http://orcid.org/0000-0002-1253-2720https://orcid.org/0000-0002-7523-7868http://orcid.org/0000-0002-2084-672Xhttp://orcid.org/0000-0002-1983-3104http://orcid.org/0000-0002-4013-0147To determine the effect of the inclusion method on the histomorphometric evaluation of the gastrointestinal mucosa of horses, jejunum samples were collected using flank laparotomy. Sixteen mixed breed healthy adult horses, including four males and 12 females, aged 4–14 years with an average body weight of 248.40 ± 2.28 kg, were used. Jejunal biopsies were collected and analyzed by light microscopy using two methods: group 1 comprised biopsies fixed using 10% neutral formalin and embedded in paraffin; biopsies in group 2 were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.2, followed by inclusion in glycol methacrylate (GMA)-based plastic resin. Intestinal villi height, crypt depth, glandular mucosa thickness, total mucosal thickness, and villus/crypt ratio were then evaluated. For all the variables studied, with exception of the villus/crypt ratio, a significant difference (p < 0.05) was found between samples in groups 1 and 2. Processing samples for embedding in plastic resin was quicker and easier to perform compared to that for paraffin embedding. In addition, the epithelial lining of tissues in group 2 showed better resolution for conducting cytological studies under a light microscope. The difference between the studied variables can be attributed to tissue retraction caused by conventional processing for inclusion in paraffin. Therefore, the method of inclusion in GMA described in the present study appears to be a more reliable choice for morphometric evaluation of the intestinal mucosa of horses.Para determinar o efeito do método de inclusão sobre a avaliação histomorfométrica da mucosa gastrointestinal de equinos foram coletadas amostras do jejuno por laparotomia pelo flanco. Foram utilizados 16 equinos adultos hígidos, sem raça definida, de ambos os sexos, quatro machos e 12 fêmeas, com idade variando entre quatro e 14 anos, e peso corporal médio de 248,40 + 2,28kg. Amostras do jejuno foram coletadas e processadas para biopsia em microscopia de luz sob dois métodos: grupo 1 - fixação em formalina neutra tamponada a 10% e inclusão em parafina, grupo 2 - fixação em glutaraldeído 2,5% em tampão fosfato 0,1M pH 7,2, seguido de inclusão em resina plástica à base de glicol metacrilato. Os seguintes parâmetros foram avaliados: altura das vilosidades intestinais, profundidade de cripta, espessura da mucosa glandular, espessura total da mucosa e relação vilo/cripta. Para todas as variáveis estudadas, exceto relação vilo/cripta, foi encontrada diferença significativa (p<0,05) entre os dois métodos. O processamento para inclusão em resina plástica foi rápido e de fácil execução quando comparado à inclusão em parafina. Além disso, o epitélio de revestimento apresentou melhor resolução das células para estudo histológico ao microscópio de luz. A diferença entre as variáveis pode ser atribuída a retração do tecido provocada pelo processamento convencional por inclusão em parafina. Portanto, o método de inclusão em GMA mostrou-se, no presente estudo, uma escolha mais fidedigna para as avaliações morfométricas da mucosa intestinal de equinos.engUniversidade Federal de Minas GeraisUFMGBrasilVET - DEPARTAMENTO DE CLÍNICA E CIRURGIABrazilian Journal of Veterinary MedicineBiópsiaEquinoMucosa intestinalIntestino delgadoBiopsyEquineIntestinal mucosaSmall intestineVilli heightComparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horsesComparação entre as técnicas de inclusão em resina plástica à base de glicol metacrilato (GMA) e inclusão em parafina para avaliação da morfometria intestinal em equinosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://bjvm.org.br/BJVM/article/view/1226Cintia FerreiraMaristela Silveira PalharesUbiratan Pereira de MeloHelio Chiarini-GarciaRenata de Pino Albuquerque MaranhãoValentim Arabicano GhellerFabiola de Oliveira Paes Lemeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/67539/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALComparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses.pdfComparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses.pdfapplication/pdf514987https://repositorio.ufmg.br/bitstream/1843/67539/2/Comparison%20between%20the%20techniques%20of%20inclusion%20in%20glycol%20methacrylate%20%28GMA%29-%20based%20plastic%20resin%20and%20paraffin%20for%20evaluation%20intestinal%20morphometry%20in%20horses.pdf4d774a88e9496a674c2eb3d81277fe61MD521843/675392024-04-22 17:57:23.392oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2024-04-22T20:57:23Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
dc.title.alternative.pt_BR.fl_str_mv |
Comparação entre as técnicas de inclusão em resina plástica à base de glicol metacrilato (GMA) e inclusão em parafina para avaliação da morfometria intestinal em equinos |
title |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
spellingShingle |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses Cintia Ferreira Biopsy Equine Intestinal mucosa Small intestine Villi height Biópsia Equino Mucosa intestinal Intestino delgado |
title_short |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
title_full |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
title_fullStr |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
title_full_unstemmed |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
title_sort |
Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses |
author |
Cintia Ferreira |
author_facet |
Cintia Ferreira Maristela Silveira Palhares Ubiratan Pereira de Melo Helio Chiarini-Garcia Renata de Pino Albuquerque Maranhão Valentim Arabicano Gheller Fabiola de Oliveira Paes Leme |
author_role |
author |
author2 |
Maristela Silveira Palhares Ubiratan Pereira de Melo Helio Chiarini-Garcia Renata de Pino Albuquerque Maranhão Valentim Arabicano Gheller Fabiola de Oliveira Paes Leme |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Cintia Ferreira Maristela Silveira Palhares Ubiratan Pereira de Melo Helio Chiarini-Garcia Renata de Pino Albuquerque Maranhão Valentim Arabicano Gheller Fabiola de Oliveira Paes Leme |
dc.subject.por.fl_str_mv |
Biopsy Equine Intestinal mucosa Small intestine Villi height |
topic |
Biopsy Equine Intestinal mucosa Small intestine Villi height Biópsia Equino Mucosa intestinal Intestino delgado |
dc.subject.other.pt_BR.fl_str_mv |
Biópsia Equino Mucosa intestinal Intestino delgado |
description |
To determine the effect of the inclusion method on the histomorphometric evaluation of the gastrointestinal mucosa of horses, jejunum samples were collected using flank laparotomy. Sixteen mixed breed healthy adult horses, including four males and 12 females, aged 4–14 years with an average body weight of 248.40 ± 2.28 kg, were used. Jejunal biopsies were collected and analyzed by light microscopy using two methods: group 1 comprised biopsies fixed using 10% neutral formalin and embedded in paraffin; biopsies in group 2 were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.2, followed by inclusion in glycol methacrylate (GMA)-based plastic resin. Intestinal villi height, crypt depth, glandular mucosa thickness, total mucosal thickness, and villus/crypt ratio were then evaluated. For all the variables studied, with exception of the villus/crypt ratio, a significant difference (p < 0.05) was found between samples in groups 1 and 2. Processing samples for embedding in plastic resin was quicker and easier to perform compared to that for paraffin embedding. In addition, the epithelial lining of tissues in group 2 showed better resolution for conducting cytological studies under a light microscope. The difference between the studied variables can be attributed to tissue retraction caused by conventional processing for inclusion in paraffin. Therefore, the method of inclusion in GMA described in the present study appears to be a more reliable choice for morphometric evaluation of the intestinal mucosa of horses. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022 |
dc.date.accessioned.fl_str_mv |
2024-04-22T20:57:22Z |
dc.date.available.fl_str_mv |
2024-04-22T20:57:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/67539 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.29374/2527-2179.bjvm004521 |
dc.identifier.issn.pt_BR.fl_str_mv |
2527-2179 |
dc.identifier.orcid.pt_BR.fl_str_mv |
http://orcid.org/0000-0003-2473-1580 http://orcid.org/0000-0002-1253-2720 https://orcid.org/0000-0002-7523-7868 http://orcid.org/0000-0002-2084-672X http://orcid.org/0000-0002-1983-3104 http://orcid.org/0000-0002-4013-0147 |
url |
https://doi.org/10.29374/2527-2179.bjvm004521 http://hdl.handle.net/1843/67539 http://orcid.org/0000-0003-2473-1580 http://orcid.org/0000-0002-1253-2720 https://orcid.org/0000-0002-7523-7868 http://orcid.org/0000-0002-2084-672X http://orcid.org/0000-0002-1983-3104 http://orcid.org/0000-0002-4013-0147 |
identifier_str_mv |
2527-2179 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian Journal of Veterinary Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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UFMG |
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UFMG |
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Repositório Institucional da UFMG |
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Repositório Institucional da UFMG |
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