Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidone

Detalhes bibliográficos
Autor(a) principal: Rummenigge Oliveira Silva
Data de Publicação: 2019
Outros Autores: Armando da Silva Cunha Júnior, Bruna Lopes da Costa, Flávia Rodrigues da Silva, Carolina Nunes da Silva, Mayara Rodrigues Brandão de Paiva, Lays Fernanda Nunes Dourado, Ângelo Malachias de Souza, Adriano Antunes de Souza Araújo, Paula Santos Nunes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1016/j.ijpharm.2019.118466
http://hdl.handle.net/1843/51758
https://orcid.org/0000-0002-4506-7570
https://orcid.org/0000-0002-1161-8936
https://orcid.org/0000-0003-3007-9795
https://orcid.org/0000-0003-1382-2487
https://orcid.org/0000-0002-6449-7904
https://orcid.org/0000-0001-5287-455X
https://orcid.org/0000-0002-8703-4283
https://orcid.org/0000-0003-3588-0178
Resumo: Some recent studies have shown that pirfenidone (PFD) has favorable results in the healing process of the cornea. However, PFD in solution exhibits short half-life after topical application, and in this context, a liquid crystal nanoparticle system containing PFD (PFD-LCNPs) was developed. The nanoparticles were characterized by transmission electron microscopy, atomic force microscopy, small angle X-ray diffraction and polarized light microscopy. The PFD-LCNPs had particle size and zeta potential of 247.3 nm and −33.60 mV (stores at 4 °C), respectively, and 257.5 nm and −46.00 mV (stored at 25 °C), respectively. The pH of the formulation was 6.9 and the encapsulation efficiency was 35.9%. The in vitro release profiles indicated that PFD sustained release from PFD-LCNPs for up to 12 h. In vitro study of ocular irritation (HET-CAM test) concluded that components of the formulation are well tolerated for ocular administration. Corneal re-epithelialization time after chemical burning was significantly reduced in rabbits treated with PFD-loaded LCNPs when compared to the group treated with a vehicle. In addition, the anti-inflammatory action of pirfenidone was observed by reducing myeloperoxidase activity (MPO) and inflammatory cells in the histology of the tissues of animals treated with PFD-LCNPs. These findings indicated that the PFD-LCNPs might have the potential for effective ocular drug delivery.
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spelling Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidoneOcular chemical burnsPirfenidoneLiquid crystalline nanoparticlesCorneaWound healingQueimaduras químicasNanopartículasCórneaCicatrização de feridasSome recent studies have shown that pirfenidone (PFD) has favorable results in the healing process of the cornea. However, PFD in solution exhibits short half-life after topical application, and in this context, a liquid crystal nanoparticle system containing PFD (PFD-LCNPs) was developed. The nanoparticles were characterized by transmission electron microscopy, atomic force microscopy, small angle X-ray diffraction and polarized light microscopy. The PFD-LCNPs had particle size and zeta potential of 247.3 nm and −33.60 mV (stores at 4 °C), respectively, and 257.5 nm and −46.00 mV (stored at 25 °C), respectively. The pH of the formulation was 6.9 and the encapsulation efficiency was 35.9%. The in vitro release profiles indicated that PFD sustained release from PFD-LCNPs for up to 12 h. In vitro study of ocular irritation (HET-CAM test) concluded that components of the formulation are well tolerated for ocular administration. Corneal re-epithelialization time after chemical burning was significantly reduced in rabbits treated with PFD-loaded LCNPs when compared to the group treated with a vehicle. In addition, the anti-inflammatory action of pirfenidone was observed by reducing myeloperoxidase activity (MPO) and inflammatory cells in the histology of the tissues of animals treated with PFD-LCNPs. These findings indicated that the PFD-LCNPs might have the potential for effective ocular drug delivery.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)FAPESP - Fundação de Amparo à Pesquisa do Estado de São PauloUniversidade Federal de Minas GeraisBrasilFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSICX - DEPARTAMENTO DE FÍSICAUFMG2023-04-10T20:02:46Z2023-04-10T20:02:46Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdfhttps://doi.org/10.1016/j.ijpharm.2019.1184661873-3476http://hdl.handle.net/1843/51758https://orcid.org/0000-0002-4506-7570https://orcid.org/0000-0002-1161-8936https://orcid.org/0000-0003-3007-9795https://orcid.org/0000-0003-1382-2487https://orcid.org/0000-0002-6449-7904https://orcid.org/0000-0001-5287-455Xhttps://orcid.org/0000-0002-8703-4283https://orcid.org/0000-0003-3588-0178engInternational Journal of PharmaceuticsRummenigge Oliveira SilvaArmando da Silva Cunha JúniorBruna Lopes da CostaFlávia Rodrigues da SilvaCarolina Nunes da SilvaMayara Rodrigues Brandão de PaivaLays Fernanda Nunes DouradoÂngelo Malachias de SouzaAdriano Antunes de Souza AraújoPaula Santos Nunesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2023-04-10T22:33:19Zoai:repositorio.ufmg.br:1843/51758Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2023-04-10T22:33:19Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidone
title Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidone
spellingShingle Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidone
Rummenigge Oliveira Silva
Ocular chemical burns
Pirfenidone
Liquid crystalline nanoparticles
Cornea
Wound healing
Queimaduras químicas
Nanopartículas
Córnea
Cicatrização de feridas
title_short Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidone
title_full Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidone
title_fullStr Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidone
title_full_unstemmed Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidone
title_sort Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidone
author Rummenigge Oliveira Silva
author_facet Rummenigge Oliveira Silva
Armando da Silva Cunha Júnior
Bruna Lopes da Costa
Flávia Rodrigues da Silva
Carolina Nunes da Silva
Mayara Rodrigues Brandão de Paiva
Lays Fernanda Nunes Dourado
Ângelo Malachias de Souza
Adriano Antunes de Souza Araújo
Paula Santos Nunes
author_role author
author2 Armando da Silva Cunha Júnior
Bruna Lopes da Costa
Flávia Rodrigues da Silva
Carolina Nunes da Silva
Mayara Rodrigues Brandão de Paiva
Lays Fernanda Nunes Dourado
Ângelo Malachias de Souza
Adriano Antunes de Souza Araújo
Paula Santos Nunes
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rummenigge Oliveira Silva
Armando da Silva Cunha Júnior
Bruna Lopes da Costa
Flávia Rodrigues da Silva
Carolina Nunes da Silva
Mayara Rodrigues Brandão de Paiva
Lays Fernanda Nunes Dourado
Ângelo Malachias de Souza
Adriano Antunes de Souza Araújo
Paula Santos Nunes
dc.subject.por.fl_str_mv Ocular chemical burns
Pirfenidone
Liquid crystalline nanoparticles
Cornea
Wound healing
Queimaduras químicas
Nanopartículas
Córnea
Cicatrização de feridas
topic Ocular chemical burns
Pirfenidone
Liquid crystalline nanoparticles
Cornea
Wound healing
Queimaduras químicas
Nanopartículas
Córnea
Cicatrização de feridas
description Some recent studies have shown that pirfenidone (PFD) has favorable results in the healing process of the cornea. However, PFD in solution exhibits short half-life after topical application, and in this context, a liquid crystal nanoparticle system containing PFD (PFD-LCNPs) was developed. The nanoparticles were characterized by transmission electron microscopy, atomic force microscopy, small angle X-ray diffraction and polarized light microscopy. The PFD-LCNPs had particle size and zeta potential of 247.3 nm and −33.60 mV (stores at 4 °C), respectively, and 257.5 nm and −46.00 mV (stored at 25 °C), respectively. The pH of the formulation was 6.9 and the encapsulation efficiency was 35.9%. The in vitro release profiles indicated that PFD sustained release from PFD-LCNPs for up to 12 h. In vitro study of ocular irritation (HET-CAM test) concluded that components of the formulation are well tolerated for ocular administration. Corneal re-epithelialization time after chemical burning was significantly reduced in rabbits treated with PFD-loaded LCNPs when compared to the group treated with a vehicle. In addition, the anti-inflammatory action of pirfenidone was observed by reducing myeloperoxidase activity (MPO) and inflammatory cells in the histology of the tissues of animals treated with PFD-LCNPs. These findings indicated that the PFD-LCNPs might have the potential for effective ocular drug delivery.
publishDate 2019
dc.date.none.fl_str_mv 2019
2023-04-10T20:02:46Z
2023-04-10T20:02:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.ijpharm.2019.118466
1873-3476
http://hdl.handle.net/1843/51758
https://orcid.org/0000-0002-4506-7570
https://orcid.org/0000-0002-1161-8936
https://orcid.org/0000-0003-3007-9795
https://orcid.org/0000-0003-1382-2487
https://orcid.org/0000-0002-6449-7904
https://orcid.org/0000-0001-5287-455X
https://orcid.org/0000-0002-8703-4283
https://orcid.org/0000-0003-3588-0178
url https://doi.org/10.1016/j.ijpharm.2019.118466
http://hdl.handle.net/1843/51758
https://orcid.org/0000-0002-4506-7570
https://orcid.org/0000-0002-1161-8936
https://orcid.org/0000-0003-3007-9795
https://orcid.org/0000-0003-1382-2487
https://orcid.org/0000-0002-6449-7904
https://orcid.org/0000-0001-5287-455X
https://orcid.org/0000-0002-8703-4283
https://orcid.org/0000-0003-3588-0178
identifier_str_mv 1873-3476
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Pharmaceutics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
Brasil
FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
ICX - DEPARTAMENTO DE FÍSICA
UFMG
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
Brasil
FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
ICX - DEPARTAMENTO DE FÍSICA
UFMG
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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