Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1590/2359-3997000000168 http://hdl.handle.net/1843/40287 |
Resumo: | Objective: To evaluate, in rat offspring, bone changes induced by excess maternal thyroxin during pregnancy and lactation, and to assess the reversibility of these changes after weaning. Material and methods: Twenty Wistar rats were distributed in two groups, hyperthyroid and control, that were treated daily with L-thyroxin (50 mcg/animal) and placebo, respectively. The treatment was initiated seven days before mating and continued throughout pregnancy and lactation. From every female of each of the two groups, two offspring were euthanized after birth, two at 21 days of age (weaning), and two at 42 days of age (21 days after weaning). In newborns, the length of pelvic and thoracic limbs were measured, and in the other animals, the length and width of the femur and humerus were measured. Bones were dissected, decalcified, embedded in paraffin, and analyzed histomorphometrically. Results: Excess maternal thyroxin significantly reduced the length of the pelvic limb in neonates. In 21-day-old individuals, excess maternal thyroxine reduced the length and the width of the femur and the humerus. It also increased thickness of the epiphyseal plate and the percentage of trabecular bone tissue. In 42-day-old individuals, there were no significant differences between groups in relation to the parameters evaluated in the previous periods. Conclusion: Excess maternal thyroxine reduced growth in suckling rats both at birth and at weaning, and it also increased the percentage of trabecular bone tissue in 21-day-old animals. These changes, however, were reversible at 42 days, i.e., 21 days after weaning. |
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2022-03-21T20:38:42Z2022-03-21T20:38:42Z2016602130137https://doi.org/10.1590/2359-399700000016823593997http://hdl.handle.net/1843/40287Objective: To evaluate, in rat offspring, bone changes induced by excess maternal thyroxin during pregnancy and lactation, and to assess the reversibility of these changes after weaning. Material and methods: Twenty Wistar rats were distributed in two groups, hyperthyroid and control, that were treated daily with L-thyroxin (50 mcg/animal) and placebo, respectively. The treatment was initiated seven days before mating and continued throughout pregnancy and lactation. From every female of each of the two groups, two offspring were euthanized after birth, two at 21 days of age (weaning), and two at 42 days of age (21 days after weaning). In newborns, the length of pelvic and thoracic limbs were measured, and in the other animals, the length and width of the femur and humerus were measured. Bones were dissected, decalcified, embedded in paraffin, and analyzed histomorphometrically. Results: Excess maternal thyroxin significantly reduced the length of the pelvic limb in neonates. In 21-day-old individuals, excess maternal thyroxine reduced the length and the width of the femur and the humerus. It also increased thickness of the epiphyseal plate and the percentage of trabecular bone tissue. In 42-day-old individuals, there were no significant differences between groups in relation to the parameters evaluated in the previous periods. Conclusion: Excess maternal thyroxine reduced growth in suckling rats both at birth and at weaning, and it also increased the percentage of trabecular bone tissue in 21-day-old animals. These changes, however, were reversible at 42 days, i.e., 21 days after weaning.Objetivo: Avaliar, na prole de ratos, as alterações ósseas induzidas pelo excesso de tiroxina materna durante gravidez e lactação, e avaliar a reversibilidade dessas alterações após o desmame. Materiais e métodos: Vinte ratos Wistar foram distribuídos em dois grupos, hipertireoide e controle, que foram tratados diariamente com L-tiroxina (50 mcg/animal) e placebo, respectivamente. O tratamento foi iniciado sete dias antes do acasalamento e continuou durante toda a gravidez e lactação. De cada fêmea de cada um dos dois grupos, dois filhotes foram sacrificados após o nascimento, dois aos 21 dias de idade (desmame), e duas aos 42 dias de idade (21 dias após o desmame). Em recém-nascidos, o comprimento dos músculos pélvico e torácico membros foram medidos, e nos demais animais, o comprimento e a largura do fêmur e do úmero foram medidos. Os ossos foram dissecados, descalcificados, incluídos em parafina e analisados histomorfometricamente. Resultados: O excesso de tiroxina materna reduziu significativamente o comprimento do membro pélvico em neonatos. Em indivíduos de 21 dias de idade, o excesso de tiroxina materna reduziu o comprimento e a largura do fêmur e do úmero. Também aumentou a espessura da placa epifisária e a porcentagem do tecido ósseo trabecular. Em indivíduos com 42 dias de idade, não houve diferenças significativas entre grupos em relação aos parâmetros avaliados nos períodos anteriores. Conclusão: Excesso materno A tiroxina reduziu o crescimento em ratas lactantes tanto ao nascimento quanto ao desmame, e também aumentou a porcentagem de tecido ósseo trabecular em animais com 21 dias de idade. Essas mudanças, no entanto, foram reversíveis em 42 dias, ou seja, 21 dias após o desmame.engUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE MORFOLOGIAICB - DEPARTAMENTO DE PATOLOGIAVET - DEPARTAMENTO DE CLÍNICA E CIRURGIAArchives of endocrinology metabolismTiroxina maternaMedicina VeterináriaCrescimento ósseoReversibilidadeRatosMaternal thyroxinbone growthreversibilityratsEffects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning periodEfeitos do excesso de tiroxina materna nos ossos da prole de ratos desde o nascimento até o período pós-desmameinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/aem/a/qcWD8CF9rzcd53ZpNxN43vk/?lang=enMariana Zanini MaiaGianne Karla SantosAna Claudia Moura BatistaAmanda Maria Sena ReisJuneo Freitas SilvaLorena Gabriela Rocha RibeiroNatália de Melo OcarinoRogéria Serakidesapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/40287/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINAL2016_Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period.pdf2016_Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period.pdfapplication/pdf184535https://repositorio.ufmg.br/bitstream/1843/40287/2/2016_Effects%20of%20excess%20maternal%20thyroxin%20on%20the%20bones%20of%20rat%20offspring%20from%20birth%20to%20the%20post-weaning%20period.pdf711b92267532bc201d20f06b3c68ea06MD521843/402872022-03-21 17:38:42.417oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-03-21T20:38:42Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period |
dc.title.alternative.pt_BR.fl_str_mv |
Efeitos do excesso de tiroxina materna nos ossos da prole de ratos desde o nascimento até o período pós-desmame |
title |
Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period |
spellingShingle |
Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period Mariana Zanini Maia Maternal thyroxin bone growth reversibility rats Tiroxina materna Medicina Veterinária Crescimento ósseo Reversibilidade Ratos |
title_short |
Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period |
title_full |
Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period |
title_fullStr |
Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period |
title_full_unstemmed |
Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period |
title_sort |
Effects of excess maternal thyroxin on the bones of rat offspring from birth to the post-weaning period |
author |
Mariana Zanini Maia |
author_facet |
Mariana Zanini Maia Gianne Karla Santos Ana Claudia Moura Batista Amanda Maria Sena Reis Juneo Freitas Silva Lorena Gabriela Rocha Ribeiro Natália de Melo Ocarino Rogéria Serakides |
author_role |
author |
author2 |
Gianne Karla Santos Ana Claudia Moura Batista Amanda Maria Sena Reis Juneo Freitas Silva Lorena Gabriela Rocha Ribeiro Natália de Melo Ocarino Rogéria Serakides |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Mariana Zanini Maia Gianne Karla Santos Ana Claudia Moura Batista Amanda Maria Sena Reis Juneo Freitas Silva Lorena Gabriela Rocha Ribeiro Natália de Melo Ocarino Rogéria Serakides |
dc.subject.por.fl_str_mv |
Maternal thyroxin bone growth reversibility rats |
topic |
Maternal thyroxin bone growth reversibility rats Tiroxina materna Medicina Veterinária Crescimento ósseo Reversibilidade Ratos |
dc.subject.other.pt_BR.fl_str_mv |
Tiroxina materna Medicina Veterinária Crescimento ósseo Reversibilidade Ratos |
description |
Objective: To evaluate, in rat offspring, bone changes induced by excess maternal thyroxin during pregnancy and lactation, and to assess the reversibility of these changes after weaning. Material and methods: Twenty Wistar rats were distributed in two groups, hyperthyroid and control, that were treated daily with L-thyroxin (50 mcg/animal) and placebo, respectively. The treatment was initiated seven days before mating and continued throughout pregnancy and lactation. From every female of each of the two groups, two offspring were euthanized after birth, two at 21 days of age (weaning), and two at 42 days of age (21 days after weaning). In newborns, the length of pelvic and thoracic limbs were measured, and in the other animals, the length and width of the femur and humerus were measured. Bones were dissected, decalcified, embedded in paraffin, and analyzed histomorphometrically. Results: Excess maternal thyroxin significantly reduced the length of the pelvic limb in neonates. In 21-day-old individuals, excess maternal thyroxine reduced the length and the width of the femur and the humerus. It also increased thickness of the epiphyseal plate and the percentage of trabecular bone tissue. In 42-day-old individuals, there were no significant differences between groups in relation to the parameters evaluated in the previous periods. Conclusion: Excess maternal thyroxine reduced growth in suckling rats both at birth and at weaning, and it also increased the percentage of trabecular bone tissue in 21-day-old animals. These changes, however, were reversible at 42 days, i.e., 21 days after weaning. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2022-03-21T20:38:42Z |
dc.date.available.fl_str_mv |
2022-03-21T20:38:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/40287 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1590/2359-3997000000168 |
dc.identifier.issn.pt_BR.fl_str_mv |
23593997 |
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https://doi.org/10.1590/2359-3997000000168 http://hdl.handle.net/1843/40287 |
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23593997 |
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eng |
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eng |
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Archives of endocrinology metabolism |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - DEPARTAMENTO DE MORFOLOGIA ICB - DEPARTAMENTO DE PATOLOGIA VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA |
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Universidade Federal de Minas Gerais |
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