Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country

Detalhes bibliográficos
Autor(a) principal: Alessandra Rejane Ericsson de Oliveira Xavier
Data de Publicação: 2019
Outros Autores: Léia Cardoso, Ronise Viviane Jorge Brito, Sérgio Avelino Mota Nobre, Anna Christina de Almeida, Alexandre Moisés Ericsson de Oliveira, Mauro Aparecido de Sousa Xavier
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: http://hdl.handle.net/1843/42274
https://orcid.org/ 0000-0001-9836-4117
Resumo: Exposure to animal feces contaminated with pathogenic microorganisms poses a risk to human health. The presence of fungi and yeasts in environments is related to adverse effects on respiratory health. An objective approach to mitigating possible health effects due to exposure to fungi and yeasts in closed environments is dificult due to the lack of guidelines. The aim of this work was, to identify and characterize microorganisms of medical importance isolated from pigeon feces collected on roof tiles of a university building and also to evaluate the hygienic-sanitary conditions of the study area. Excrement sampling points were established, and the microorganisms were identiied by standard biochemical and microbiological methods followed by MALDI-TOF proteomics for identiication at the species level. Microbial identiication revealed the presence of medically important microorganisms in the samples analyzed, namely the fungi Candida krusei, Candida rugosa, Aspergillus spp., Cryptococcus neoformans and Trichosporon asahii and bacterium Klebsiela pneumoniae. The results obtained demonstrated the need for the development of methods that help mitigate risks to human health due to exposure to pathogenic agents in environments such as that studied.
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spelling 2022-06-06T15:12:44Z2022-06-06T15:12:44Z20193211120Http://dx.doi.org/10.5007/2175-7925.2019v32n1p112175-7925http://hdl.handle.net/1843/42274https://orcid.org/ 0000-0001-9836-4117Exposure to animal feces contaminated with pathogenic microorganisms poses a risk to human health. The presence of fungi and yeasts in environments is related to adverse effects on respiratory health. An objective approach to mitigating possible health effects due to exposure to fungi and yeasts in closed environments is dificult due to the lack of guidelines. The aim of this work was, to identify and characterize microorganisms of medical importance isolated from pigeon feces collected on roof tiles of a university building and also to evaluate the hygienic-sanitary conditions of the study area. Excrement sampling points were established, and the microorganisms were identiied by standard biochemical and microbiological methods followed by MALDI-TOF proteomics for identiication at the species level. Microbial identiication revealed the presence of medically important microorganisms in the samples analyzed, namely the fungi Candida krusei, Candida rugosa, Aspergillus spp., Cryptococcus neoformans and Trichosporon asahii and bacterium Klebsiela pneumoniae. The results obtained demonstrated the need for the development of methods that help mitigate risks to human health due to exposure to pathogenic agents in environments such as that studied.A exposição humana a fezes de animais contaminados com microrganismos patogênicos representa um risco para a saúde. A presença de fungos e leveduras em ambientes está relacionada a efeitos adversos na saúde respiratória. A abordagem objetiva para a mitigação de possíveis efeitos à saúde devido à exposição a fungos e leveduras em ambientes fechados é difícil devido à falta de diretrizes. O objetivo deste trabalho foi conhecer as condições higiênico-sanitárias, identiicar e caracterizar microorganismos de importância médica isolados de fezes de pombos coletadas na laje de um telhado de um prédio público. Para tais pontos, a coleta de excrementos foi estabelecida e os microrganismos foram identiicados por métodos bioquímicos e microbiológicos padronizados, seguidos da abordagem proteômica de MALDI-TOF para a identiicação do nível de espécie. Os resultados da identiicação microbiana revelaram a presença de microrganismos de importância médica nas amostras analisadas, a saber: Candida krusei, Candida rugosa, Aspergillus spp., Cryptococcus neoformans, Klebsiella pneumoniae e Trichosporon asahii. Os resultados encontrados revelam a necessidade do desenvolvimento de metodologias que permitam mitigar os riscos à saúde humana devido à exposição a agentes patogênicos no ambiente examinado.Outra AgênciaengUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASBiotemasMicrorganismosMicrorganismos -- ContaminaçãoFezesDetection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized countryDetecção e identiicação de microrganismos de importância médica isolados em fezes de pombos coletados em uma universidade de um país recém-industrializadoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://core.ac.uk/display/274118455Alessandra Rejane Ericsson de Oliveira XavierLéia CardosoRonise Viviane Jorge BritoSérgio Avelino Mota NobreAnna Christina de AlmeidaAlexandre Moisés Ericsson de OliveiraMauro Aparecido de Sousa Xavierinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/42274/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALDETECT~1.PDFDETECT~1.PDFDetection and identiication of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized countryapplication/pdf2268524https://repositorio.ufmg.br/bitstream/1843/42274/2/DETECT~1.PDF86dadd43724b7c62b5b9b002692fca8dMD521843/422742022-06-06 12:12:45.054oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-06-06T15:12:45Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country
dc.title.alternative.pt_BR.fl_str_mv Detecção e identiicação de microrganismos de importância médica isolados em fezes de pombos coletados em uma universidade de um país recém-industrializado
title Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country
spellingShingle Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country
Alessandra Rejane Ericsson de Oliveira Xavier
Microrganismos
Microrganismos -- Contaminação
Fezes
title_short Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country
title_full Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country
title_fullStr Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country
title_full_unstemmed Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country
title_sort Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country
author Alessandra Rejane Ericsson de Oliveira Xavier
author_facet Alessandra Rejane Ericsson de Oliveira Xavier
Léia Cardoso
Ronise Viviane Jorge Brito
Sérgio Avelino Mota Nobre
Anna Christina de Almeida
Alexandre Moisés Ericsson de Oliveira
Mauro Aparecido de Sousa Xavier
author_role author
author2 Léia Cardoso
Ronise Viviane Jorge Brito
Sérgio Avelino Mota Nobre
Anna Christina de Almeida
Alexandre Moisés Ericsson de Oliveira
Mauro Aparecido de Sousa Xavier
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alessandra Rejane Ericsson de Oliveira Xavier
Léia Cardoso
Ronise Viviane Jorge Brito
Sérgio Avelino Mota Nobre
Anna Christina de Almeida
Alexandre Moisés Ericsson de Oliveira
Mauro Aparecido de Sousa Xavier
dc.subject.other.pt_BR.fl_str_mv Microrganismos
Microrganismos -- Contaminação
Fezes
topic Microrganismos
Microrganismos -- Contaminação
Fezes
description Exposure to animal feces contaminated with pathogenic microorganisms poses a risk to human health. The presence of fungi and yeasts in environments is related to adverse effects on respiratory health. An objective approach to mitigating possible health effects due to exposure to fungi and yeasts in closed environments is dificult due to the lack of guidelines. The aim of this work was, to identify and characterize microorganisms of medical importance isolated from pigeon feces collected on roof tiles of a university building and also to evaluate the hygienic-sanitary conditions of the study area. Excrement sampling points were established, and the microorganisms were identiied by standard biochemical and microbiological methods followed by MALDI-TOF proteomics for identiication at the species level. Microbial identiication revealed the presence of medically important microorganisms in the samples analyzed, namely the fungi Candida krusei, Candida rugosa, Aspergillus spp., Cryptococcus neoformans and Trichosporon asahii and bacterium Klebsiela pneumoniae. The results obtained demonstrated the need for the development of methods that help mitigate risks to human health due to exposure to pathogenic agents in environments such as that studied.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2022-06-06T15:12:44Z
dc.date.available.fl_str_mv 2022-06-06T15:12:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/42274
dc.identifier.doi.pt_BR.fl_str_mv Http://dx.doi.org/10.5007/2175-7925.2019v32n1p11
dc.identifier.issn.pt_BR.fl_str_mv 2175-7925
dc.identifier.orcid.pt_BR.fl_str_mv https://orcid.org/ 0000-0001-9836-4117
identifier_str_mv Http://dx.doi.org/10.5007/2175-7925.2019v32n1p11
2175-7925
url http://hdl.handle.net/1843/42274
https://orcid.org/ 0000-0001-9836-4117
dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Biotemas
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
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