Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/42274 https://orcid.org/ 0000-0001-9836-4117 |
Resumo: | Exposure to animal feces contaminated with pathogenic microorganisms poses a risk to human health. The presence of fungi and yeasts in environments is related to adverse effects on respiratory health. An objective approach to mitigating possible health effects due to exposure to fungi and yeasts in closed environments is dificult due to the lack of guidelines. The aim of this work was, to identify and characterize microorganisms of medical importance isolated from pigeon feces collected on roof tiles of a university building and also to evaluate the hygienic-sanitary conditions of the study area. Excrement sampling points were established, and the microorganisms were identiied by standard biochemical and microbiological methods followed by MALDI-TOF proteomics for identiication at the species level. Microbial identiication revealed the presence of medically important microorganisms in the samples analyzed, namely the fungi Candida krusei, Candida rugosa, Aspergillus spp., Cryptococcus neoformans and Trichosporon asahii and bacterium Klebsiela pneumoniae. The results obtained demonstrated the need for the development of methods that help mitigate risks to human health due to exposure to pathogenic agents in environments such as that studied. |
id |
UFMG_ef0f2a5198271aba5b05648f2148dbc1 |
---|---|
oai_identifier_str |
oai:repositorio.ufmg.br:1843/42274 |
network_acronym_str |
UFMG |
network_name_str |
Repositório Institucional da UFMG |
repository_id_str |
|
spelling |
2022-06-06T15:12:44Z2022-06-06T15:12:44Z20193211120Http://dx.doi.org/10.5007/2175-7925.2019v32n1p112175-7925http://hdl.handle.net/1843/42274https://orcid.org/ 0000-0001-9836-4117Exposure to animal feces contaminated with pathogenic microorganisms poses a risk to human health. The presence of fungi and yeasts in environments is related to adverse effects on respiratory health. An objective approach to mitigating possible health effects due to exposure to fungi and yeasts in closed environments is dificult due to the lack of guidelines. The aim of this work was, to identify and characterize microorganisms of medical importance isolated from pigeon feces collected on roof tiles of a university building and also to evaluate the hygienic-sanitary conditions of the study area. Excrement sampling points were established, and the microorganisms were identiied by standard biochemical and microbiological methods followed by MALDI-TOF proteomics for identiication at the species level. Microbial identiication revealed the presence of medically important microorganisms in the samples analyzed, namely the fungi Candida krusei, Candida rugosa, Aspergillus spp., Cryptococcus neoformans and Trichosporon asahii and bacterium Klebsiela pneumoniae. The results obtained demonstrated the need for the development of methods that help mitigate risks to human health due to exposure to pathogenic agents in environments such as that studied.A exposição humana a fezes de animais contaminados com microrganismos patogênicos representa um risco para a saúde. A presença de fungos e leveduras em ambientes está relacionada a efeitos adversos na saúde respiratória. A abordagem objetiva para a mitigação de possíveis efeitos à saúde devido à exposição a fungos e leveduras em ambientes fechados é difícil devido à falta de diretrizes. O objetivo deste trabalho foi conhecer as condições higiênico-sanitárias, identiicar e caracterizar microorganismos de importância médica isolados de fezes de pombos coletadas na laje de um telhado de um prédio público. Para tais pontos, a coleta de excrementos foi estabelecida e os microrganismos foram identiicados por métodos bioquímicos e microbiológicos padronizados, seguidos da abordagem proteômica de MALDI-TOF para a identiicação do nível de espécie. Os resultados da identiicação microbiana revelaram a presença de microrganismos de importância médica nas amostras analisadas, a saber: Candida krusei, Candida rugosa, Aspergillus spp., Cryptococcus neoformans, Klebsiella pneumoniae e Trichosporon asahii. Os resultados encontrados revelam a necessidade do desenvolvimento de metodologias que permitam mitigar os riscos à saúde humana devido à exposição a agentes patogênicos no ambiente examinado.Outra AgênciaengUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASBiotemasMicrorganismosMicrorganismos -- ContaminaçãoFezesDetection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized countryDetecção e identiicação de microrganismos de importância médica isolados em fezes de pombos coletados em uma universidade de um país recém-industrializadoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://core.ac.uk/display/274118455Alessandra Rejane Ericsson de Oliveira XavierLéia CardosoRonise Viviane Jorge BritoSérgio Avelino Mota NobreAnna Christina de AlmeidaAlexandre Moisés Ericsson de OliveiraMauro Aparecido de Sousa Xavierinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/42274/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALDETECT~1.PDFDETECT~1.PDFDetection and identiication of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized countryapplication/pdf2268524https://repositorio.ufmg.br/bitstream/1843/42274/2/DETECT~1.PDF86dadd43724b7c62b5b9b002692fca8dMD521843/422742022-06-06 12:12:45.054oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-06-06T15:12:45Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country |
dc.title.alternative.pt_BR.fl_str_mv |
Detecção e identiicação de microrganismos de importância médica isolados em fezes de pombos coletados em uma universidade de um país recém-industrializado |
title |
Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country |
spellingShingle |
Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country Alessandra Rejane Ericsson de Oliveira Xavier Microrganismos Microrganismos -- Contaminação Fezes |
title_short |
Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country |
title_full |
Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country |
title_fullStr |
Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country |
title_full_unstemmed |
Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country |
title_sort |
Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country |
author |
Alessandra Rejane Ericsson de Oliveira Xavier |
author_facet |
Alessandra Rejane Ericsson de Oliveira Xavier Léia Cardoso Ronise Viviane Jorge Brito Sérgio Avelino Mota Nobre Anna Christina de Almeida Alexandre Moisés Ericsson de Oliveira Mauro Aparecido de Sousa Xavier |
author_role |
author |
author2 |
Léia Cardoso Ronise Viviane Jorge Brito Sérgio Avelino Mota Nobre Anna Christina de Almeida Alexandre Moisés Ericsson de Oliveira Mauro Aparecido de Sousa Xavier |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Alessandra Rejane Ericsson de Oliveira Xavier Léia Cardoso Ronise Viviane Jorge Brito Sérgio Avelino Mota Nobre Anna Christina de Almeida Alexandre Moisés Ericsson de Oliveira Mauro Aparecido de Sousa Xavier |
dc.subject.other.pt_BR.fl_str_mv |
Microrganismos Microrganismos -- Contaminação Fezes |
topic |
Microrganismos Microrganismos -- Contaminação Fezes |
description |
Exposure to animal feces contaminated with pathogenic microorganisms poses a risk to human health. The presence of fungi and yeasts in environments is related to adverse effects on respiratory health. An objective approach to mitigating possible health effects due to exposure to fungi and yeasts in closed environments is dificult due to the lack of guidelines. The aim of this work was, to identify and characterize microorganisms of medical importance isolated from pigeon feces collected on roof tiles of a university building and also to evaluate the hygienic-sanitary conditions of the study area. Excrement sampling points were established, and the microorganisms were identiied by standard biochemical and microbiological methods followed by MALDI-TOF proteomics for identiication at the species level. Microbial identiication revealed the presence of medically important microorganisms in the samples analyzed, namely the fungi Candida krusei, Candida rugosa, Aspergillus spp., Cryptococcus neoformans and Trichosporon asahii and bacterium Klebsiela pneumoniae. The results obtained demonstrated the need for the development of methods that help mitigate risks to human health due to exposure to pathogenic agents in environments such as that studied. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2022-06-06T15:12:44Z |
dc.date.available.fl_str_mv |
2022-06-06T15:12:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/42274 |
dc.identifier.doi.pt_BR.fl_str_mv |
Http://dx.doi.org/10.5007/2175-7925.2019v32n1p11 |
dc.identifier.issn.pt_BR.fl_str_mv |
2175-7925 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/ 0000-0001-9836-4117 |
identifier_str_mv |
Http://dx.doi.org/10.5007/2175-7925.2019v32n1p11 2175-7925 |
url |
http://hdl.handle.net/1843/42274 https://orcid.org/ 0000-0001-9836-4117 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Biotemas |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Repositório Institucional da UFMG |
collection |
Repositório Institucional da UFMG |
bitstream.url.fl_str_mv |
https://repositorio.ufmg.br/bitstream/1843/42274/1/License.txt https://repositorio.ufmg.br/bitstream/1843/42274/2/DETECT~1.PDF |
bitstream.checksum.fl_str_mv |
fa505098d172de0bc8864fc1287ffe22 86dadd43724b7c62b5b9b002692fca8d |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
|
_version_ |
1801676759634018304 |