Body mass index and the visceral adipose tissue expression of IL-6 and TNF-alpha are associated with the morphological severity of non-alcoholic fatty liver disease in individuals with class III obesity

• Autor(a) principal: Antônio Sérgio Barcala Jorge
• Data de Publicação: 2018
• Outros Autores: João Marcus Oliveira Andrade, Alanna Fernandes Paraíso, Gislaine Cândida Batista Jorge, Christine Mendes Silveira, Ludmilla Regina de Souza, Erivelton Pereira Santos, André Luiz Sena Guimarães, Sergio Henrique Sousa Santos, Alfredo Mauricio Batista de Paula
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.1016/j.orcp.2016.03.009; http://hdl.handle.net/1843/44304
• topic: Índice de massa corporal; Tecido adiposo; Obesidade; Fígado - Doenças; Citocinas

Objectives: to analyze the mRNA expression of interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α) in the liver and white adipose tissue samples of individuals with class III obesity (body mass index ≥40.0 kg/m2) with non-alcoholic fatty liver disease (NAFLD). Methods: this cross-sectional study included patients with class III obesity exhibiting early or late morphological presentation of NAFLD (non-alcoholic hepatic steatosis [NAFL], n = 8 and non-alcoholic steatohepatitis [NASH], n = 13, respectively). All patients underwent bariatric surgery and peripheral blood, liver, and visceral white adipose tissue (WAT) samples were collected. Socio-demographic, anthropometric, clinical, plasma biochemical, and nutritional characteristics of each study subject were assessed and compared between patients presenting with NAFL and NASH. IL-6 and TNF-α mRNA expression in the liver and WAT samples were measured by using quantitative real time-polymerase chain reaction (qRT-PCR). Results: individuals with class III obesity and NASH showed higher body mass index (BMI) and higher IL-6 and TNF-α mRNA expression in the WAT compared to that of patients with NAFL (p = 0.01, for all associations). Conclusions: individuals with class III obesity with higher morphological severity of NAFLD exhibited higher BMI and higher IL-6 and TNF-α expression in the WAT. Future prospective studies are warranted to determine how BMI, IL-6, and TNF-α affect the progression of NAFLD in individuals with class III obesity.