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2022-08-12T17:11:20Z2022-08-12T17:11:20Z20162448-0002http://hdl.handle.net/1843/44231The cells of the gastrointestinal epithelial mucosa are constantly under high regenerative pressure, due to the expeditious rate of cell turnover in this tissue. To regulate homeostasis in the gastrointestinal tract, a vital balance between cell apoptosis, senescence and the proliferation and differentiation of new cells must be maintained. The present research aimed was analyse the performance of the retinoblastoma protein in different parts of the stomach from neonate dogs. Were used forty five canine neonates, obtained at private veterinary clinics and Veterinary Hospital of Federal University of Piauí. The animals were from females with dystocia or pregnant, at the end of gestational stage, who had problems during parturition and/or cesarean section whose puppies had died. From these animals were obtained stomach fragments (about 0.5cm) that were fixed in buffered formaldehyde and submitted to histological routine, stained with hematoxylin and eosin, for light microscopy. Then the best blocks were submitted a immunohistochemistry process, using anti-body pRb. The data presented in this paper, strongly suggest that the Rb protein is coupled with apoptosis directly related to the number of cells and renewal of epithelial regulation and gastric mucosa.As células da mucosa epitelial gastrointestinal estão constantemente sob alta pressão regenerativa, devido à rápida taxa de renovação celular neste tecido. Para regular a homeostase no trato gastrointestinal, um equilíbrio vital entre apoptose celular, senescência e proliferação e diferenciação de novas células deve ser mantido. A presente pesquisa teve como objetivo analisar a atuação da proteína retinoblastoma em diferentes partes do estômago de cães neonatos. Foram utilizados quarenta e cinco neonatos caninos, obtidos em clínicas veterinárias particulares e Hospital Veterinário da Universidade Federal do Piauí. Os animais eram de fêmeas com distocia ou prenhes, em final de gestação, que tiveram problemas durante o parto e/ou cesariana cujos filhotes haviam morrido. Destes animais foram obtidos fragmentos de estômago (cerca de 0,5cm) que foram fixados em formol tamponado e submetidos à rotina histológica, corados com hematoxilina e eosina, para microscopia de luz. Em seguida, os melhores blocos foram submetidos a um processo de imuno-histoquímica, utilizando o anticorpo pRb. Os dados apresentados neste trabalho, sugerem fortemente que a proteína Rb está acoplada à apoptose diretamente relacionada ao número de células e renovação da regulação epitelial e da mucosa gástrica.engUniversidade Federal de Minas GeraisUFMGBrasilICB - INSTITUTO DE CIÊNCIAS BIOLOGICASJornal Interdisciplinar de BiociênciasImuno-histoquímicaProteína de RetinoblastomaTrato DigestivoEstômagoCanino NeonatoPRB expression in stomach of neonate canine without establish breedExpressão de PRB no estômago de neonatos caninos sem raça estabelecidainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://revistas.ufpi.br/index.php/jibi/article/view/4269Maria Michele Araújo de Sousa CavalcanteAndrezza Braga Soares da SilvaHeliana de Barros FernandesLuana de Oliveira LopesIngrid Macedo de OliveiraHelena Lobo BorgesAna Maria QuessadaMarcia Dos Santos RizzoAírton Mendes Conde Júniorapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/44231/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINAL2016_Prb expression in stomach of neonate canine without establish breed.pdf2016_Prb expression in stomach of neonate canine without establish breed.pdfapplication/pdf496464https://repositorio.ufmg.br/bitstream/1843/44231/2/2016_Prb%20expression%20in%20stomach%20of%20neonate%20canine%20without%20establish%20breed.pdf18c2a9d76809435652400fb4f8a339a5MD521843/442312022-08-12 14:11:20.484oai:repositorio.ufmg.br:1843/44231TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-08-12T17:11:20Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
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