Prevention of bone infection after open fracture using a chitosan with ciprofloxacin implant in animal model

• Autor(a) principal: Silvia Iovine Kobata
• Data de Publicação: 2020
• Outros Autores: Luiz Eduardo Moreira Teixeira, Simone Odília Antunes Fernandes, André Augusto Gomes Faraco, Paula Vieira Teixeira Vidigal, Ivana Duval de Araújo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: http://hdl.handle.net/1843/40943
• topic: Osteitis; Chitosan; Ciprofloxacin; Fractures; Open; Rats; Infecção óssea; Quitosana; Ciprofloxacina

Purpose To evaluate different concentrations of ciprofloxacin to prevent infection after open fracture contaminated with S. aureus in rats using absorbable local delivery system. Methods Fifty-two Wistar rats were assigned to six groups. After 4 weeks, all animals underwent 99mTc-ceftizoxima scintigraphy evaluation, callus formation measurement and histological analysis. ANOVA, t-Student and Kruskal Wallis were used for quantitative variables statistical analysis, whereas qui square and exact Fisher were used for qualitative variables. Results Treatment using 25% and 50% of ciprofloxacin incorporated at the fracture fixation device were effective in preventing bone infection compared to control group (p<0.05). Chitosan were not effective in preventing bone infection when used alone compared to control group (p>0.05). Histological findings demonstrated bone-healing delay with 50% of ciprofloxacin. No difference in callus formation were observed (p>0.05). Conclusion Local delivery treatment for contaminated open fracture using chitosan with ciprofloxacin is effective above 25%.