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Carolina Cavaliéri Gomeshttp://lattes.cnpq.br/9793295296026544Lucyene Miguita LuizElisa Carvalho de SiqueiraVanessa de Fátima Bernardeshttp://lattes.cnpq.br/9695588750342182Raphaela Alvarenga Braga de Freitas2022-09-28T19:07:17Z2022-09-28T19:07:17Z2022-07-07http://hdl.handle.net/1843/45689https://orcid.org/0000-0002-2498-3449O granuloma central de células gigantes (GCCG) dos maxilares é uma lesão intraóssea benigna, que pode apresentar um curso localmente agressivo. Setenta por cento dessas lesões apresentam mutações em TRPV4, KRAS ou FGFR1. Alguns estudos apontam que a população de células mononucleares parece ser o componente proliferativo da lesão. Essa população de células mononucleares é uma população mista, composta tanto por células mononucleares de origem monocítica quanto por células mesenquimais indiferenciadas. Assim, este estudo avaliou se, quando colocadas em cultura, o componente proliferativo da lesão é composto por células de origem mesenquimais ou de natureza monocítica. Foi estabelecida a cultura de células primárias de GCCG, a partir de uma amostra de conveniência composta por uma linhagem oriunda de uma lesão em mandíbula em paciente do sexo masculino, 20 anos. E, as amostras foram incubadas com os anticorpos CD14 e CD51/CD61, e triplicadas amostrais foram submetidas a citometria de fluxo para identificação das subpopulações de células presentes. Foi observado que somente as células mononucleares permaneciam ao longo das passagens. Pela citometria de fluxo, observou-se predominância de células CD14-CD51-CD61- triplamente negativas, compatível com o perfil esperado para células estromais/mesenquimais. Com base nos resultados, reforça-se a ideia de que as células mononucleares CD14- CD51-CD61- são centrais na patogênese dos GCCG, enquanto as células mononucleares de origem monocítica (CD14+) e as células gigantes semelhantes a osteoclastos (CD51+CD61+) são reativas.Central giant cell granuloma (CGCG) of the jaws is a benign intraosseous lesion, which may present an aggressive course. Seventy per cent of these lesions present mutations in TRPV4, KRAS or FGFR1. The population of mononuclear cells seems to be the proliferative component of the lesion. This mononuclear cell population is a mixed population, composed of both mononuclear cells of monocytic origin and undifferentiated mesenchymal cells. Thus, this study evaluated whether, when placed in culture, the proliferative component of (CGCG) is composed of mesenchymal cells or monocytic cells. The culture of primary CGCG cells was established from a convenience sample composed of a lineage originated from a mandibular lesion in 20 y.o. male patient. The samples were incubated with CD14 and CD51/CD61 antibodies, and triplicate samples were submitted to flow cytometry to identify the subpopulations of cells. It was observed that only mononuclear cells remained along the passages. By flow cytometry, a predominance of triple negative CD14-CD51-CD61- cells was observed, compatible with the expected profile for stromal/mesenchymal cells. Our results reinforce the idea that the mesenchymal cells (CD14-CD51-CD61- ) have central importance in the CGCG pathogenesis, while the mononuclear cells of monocytic origin (CD14+) and the osteoclast-like giant cells (CD51+CD61+) are reactive.CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorporUniversidade Federal de Minas GeraisPrograma de Pós-Graduação em OdontologiaUFMGBrasilFAO - FACULDADE DE ODONTOLOGIAhttp://creativecommons.org/licenses/by-nc-nd/3.0/pt/info:eu-repo/semantics/openAccessGranuloma de células gigantesCélulas gigantesOsteoclastosCitometria de fluxoCélulas-tronco mesenquimaisGranuloma de células gigantesCélulas gigantesCélulas mesenquimaisOsteoclastosCitometria de fluxoCaracterização das subpopulações de células mononucleares dos granulomas centrais de células gigantes dos maxilaresCharacterization of mononuclear cell subpopulations of central giant cell granulomas of the jawsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGORIGINALCaracterização das subpopulações de células mononucleares dos granulomas centrais de células gigantes dos maxilares.pdfCaracterização das subpopulações de células mononucleares dos granulomas centrais de células gigantes dos maxilares.pdfDissertação de Mestrado_Raphaela Alvarengaapplication/pdf689998https://repositorio.ufmg.br/bitstream/1843/45689/1/Caracteriza%c3%a7%c3%a3o%20das%20subpopula%c3%a7%c3%b5es%20de%20c%c3%a9lulas%20mononucleares%20dos%20granulomas%20centrais%20de%20c%c3%a9lulas%20gigantes%20dos%20maxilares.pdf1c42c51099b7282abe9c6406a0d5e761MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufmg.br/bitstream/1843/45689/2/license_rdfcfd6801dba008cb6adbd9838b81582abMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82118https://repositorio.ufmg.br/bitstream/1843/45689/3/license.txtcda590c95a0b51b4d15f60c9642ca272MD531843/456892023-01-13 17:59:34.395oai:repositorio.ufmg.br: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ório InstitucionalPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-01-13T20:59:34Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
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