Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children

Detalhes bibliográficos
Autor(a) principal: Brustoloni, Yvone Maia
Data de Publicação: 2007
Outros Autores: Lima, Rosimar Batista, Cunha, Rivaldo Venâncio da, Dorval, Maria Elizabeth, Oshiro, Elisa Teruya, Oliveira, Ana Lúcia Lyrio de, Pirmez, Claude
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMS
Texto Completo: https://repositorio.ufms.br/handle/123456789/1415
http://dx.doi.org/10.1590/S0074-02762007005000036.
Resumo: The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in archived Giemsa-stained bone marrow slides for diagnosis of visceral leishmaniasis (VL), and to compare PCR with conventional diagnostic techniques, like direct microscopy and parasite culture. Specimens of archived Giemsa-stained bone marrow slides from 91 patients with VL and from 79 controls with other diseases or conditions were studied. PCR showed the highest sensitivity (92.3%) and had good specificity (97.5%). Direct examination detected 79.1% and culture 59% of positive samples. In addition, PCR was able to detect VL in 16 of 19 patients (84.2%) with negative microscopy. PCR in Giemsa-stained bone marrow slides is a suitable tool for confirming diagnosis in patients with VL and may be useful in the diagnosis of difficult cases. Slide smears are easily stored, do not require special storage conditions such as low temperatures, and can be easily mailed to centers where PCR is available, making it an excellent option for diagnosis in the field.
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spelling 2012-05-22T18:50:27Z2021-09-30T19:55:10Z2007-05BRUSTOLONI, Yvone Maia et al . Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 102, n. 4, June 2007 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000400011&lng=en&nrm=iso>. access on 22 May 2012. Epub May 03, 2007.https://repositorio.ufms.br/handle/123456789/1415http://dx.doi.org/10.1590/S0074-02762007005000036.The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in archived Giemsa-stained bone marrow slides for diagnosis of visceral leishmaniasis (VL), and to compare PCR with conventional diagnostic techniques, like direct microscopy and parasite culture. Specimens of archived Giemsa-stained bone marrow slides from 91 patients with VL and from 79 controls with other diseases or conditions were studied. PCR showed the highest sensitivity (92.3%) and had good specificity (97.5%). Direct examination detected 79.1% and culture 59% of positive samples. In addition, PCR was able to detect VL in 16 of 19 patients (84.2%) with negative microscopy. PCR in Giemsa-stained bone marrow slides is a suitable tool for confirming diagnosis in patients with VL and may be useful in the diagnosis of difficult cases. Slide smears are easily stored, do not require special storage conditions such as low temperatures, and can be easily mailed to centers where PCR is available, making it an excellent option for diagnosis in the field.engMemórias do Instituto Oswaldo CruzLeishmaniose VisceralReação em Cadeia da PolimeraseDiagnósticoVisceral LeishmaniasisPolymerase Chain ReactionDiagnosisSensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in childreninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrustoloni, Yvone MaiaLima, Rosimar BatistaCunha, Rivaldo Venâncio daDorval, Maria ElizabethOshiro, Elisa TeruyaOliveira, Ana Lúcia Lyrio dePirmez, Claudeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSTHUMBNAILSensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children.pdf.jpgSensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children.pdf.jpgGenerated Thumbnailimage/jpeg1676https://repositorio.ufms.br/bitstream/123456789/1415/4/Sensitivity%20and%20specificity%20of%20polymerase%20chain%20reaction%20in%20Giemsa-stained%20slides%20for%20diagnosis%20of%20visceral%20leishmaniasis%20in%20children.pdf.jpg11100a3572f73553ab56568da8996133MD54ORIGINALSensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children.pdfSensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children.pdfapplication/pdf268541https://repositorio.ufms.br/bitstream/123456789/1415/1/Sensitivity%20and%20specificity%20of%20polymerase%20chain%20reaction%20in%20Giemsa-stained%20slides%20for%20diagnosis%20of%20visceral%20leishmaniasis%20in%20children.pdfa1bb41b9b9cdde9de4ae873f90249dbaMD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children
title Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children
spellingShingle Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children
Brustoloni, Yvone Maia
Leishmaniose Visceral
Reação em Cadeia da Polimerase
Diagnóstico
Visceral Leishmaniasis
Polymerase Chain Reaction
Diagnosis
title_short Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children
title_full Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children
title_fullStr Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children
title_full_unstemmed Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children
title_sort Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children
author Brustoloni, Yvone Maia
author_facet Brustoloni, Yvone Maia
Lima, Rosimar Batista
Cunha, Rivaldo Venâncio da
Dorval, Maria Elizabeth
Oshiro, Elisa Teruya
Oliveira, Ana Lúcia Lyrio de
Pirmez, Claude
author_role author
author2 Lima, Rosimar Batista
Cunha, Rivaldo Venâncio da
Dorval, Maria Elizabeth
Oshiro, Elisa Teruya
Oliveira, Ana Lúcia Lyrio de
Pirmez, Claude
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Brustoloni, Yvone Maia
Lima, Rosimar Batista
Cunha, Rivaldo Venâncio da
Dorval, Maria Elizabeth
Oshiro, Elisa Teruya
Oliveira, Ana Lúcia Lyrio de
Pirmez, Claude
dc.subject.por.fl_str_mv Leishmaniose Visceral
Reação em Cadeia da Polimerase
Diagnóstico
Visceral Leishmaniasis
Polymerase Chain Reaction
Diagnosis
topic Leishmaniose Visceral
Reação em Cadeia da Polimerase
Diagnóstico
Visceral Leishmaniasis
Polymerase Chain Reaction
Diagnosis
description The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in archived Giemsa-stained bone marrow slides for diagnosis of visceral leishmaniasis (VL), and to compare PCR with conventional diagnostic techniques, like direct microscopy and parasite culture. Specimens of archived Giemsa-stained bone marrow slides from 91 patients with VL and from 79 controls with other diseases or conditions were studied. PCR showed the highest sensitivity (92.3%) and had good specificity (97.5%). Direct examination detected 79.1% and culture 59% of positive samples. In addition, PCR was able to detect VL in 16 of 19 patients (84.2%) with negative microscopy. PCR in Giemsa-stained bone marrow slides is a suitable tool for confirming diagnosis in patients with VL and may be useful in the diagnosis of difficult cases. Slide smears are easily stored, do not require special storage conditions such as low temperatures, and can be easily mailed to centers where PCR is available, making it an excellent option for diagnosis in the field.
publishDate 2007
dc.date.issued.fl_str_mv 2007-05
dc.date.accessioned.fl_str_mv 2012-05-22T18:50:27Z
dc.date.available.fl_str_mv 2021-09-30T19:55:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BRUSTOLONI, Yvone Maia et al . Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 102, n. 4, June 2007 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000400011&lng=en&nrm=iso>. access on 22 May 2012. Epub May 03, 2007.
dc.identifier.uri.fl_str_mv https://repositorio.ufms.br/handle/123456789/1415
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1590/S0074-02762007005000036.
identifier_str_mv BRUSTOLONI, Yvone Maia et al . Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 102, n. 4, June 2007 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000400011&lng=en&nrm=iso>. access on 22 May 2012. Epub May 03, 2007.
url https://repositorio.ufms.br/handle/123456789/1415
http://dx.doi.org/10.1590/S0074-02762007005000036.
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dc.publisher.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
publisher.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMS
instname:Universidade Federal de Mato Grosso do Sul (UFMS)
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