Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMS |
Texto Completo: | https://repositorio.ufms.br/handle/123456789/1415 http://dx.doi.org/10.1590/S0074-02762007005000036. |
Resumo: | The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in archived Giemsa-stained bone marrow slides for diagnosis of visceral leishmaniasis (VL), and to compare PCR with conventional diagnostic techniques, like direct microscopy and parasite culture. Specimens of archived Giemsa-stained bone marrow slides from 91 patients with VL and from 79 controls with other diseases or conditions were studied. PCR showed the highest sensitivity (92.3%) and had good specificity (97.5%). Direct examination detected 79.1% and culture 59% of positive samples. In addition, PCR was able to detect VL in 16 of 19 patients (84.2%) with negative microscopy. PCR in Giemsa-stained bone marrow slides is a suitable tool for confirming diagnosis in patients with VL and may be useful in the diagnosis of difficult cases. Slide smears are easily stored, do not require special storage conditions such as low temperatures, and can be easily mailed to centers where PCR is available, making it an excellent option for diagnosis in the field. |
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2012-05-22T18:50:27Z2021-09-30T19:55:10Z2007-05BRUSTOLONI, Yvone Maia et al . Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 102, n. 4, June 2007 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000400011&lng=en&nrm=iso>. access on 22 May 2012. Epub May 03, 2007.https://repositorio.ufms.br/handle/123456789/1415http://dx.doi.org/10.1590/S0074-02762007005000036.The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in archived Giemsa-stained bone marrow slides for diagnosis of visceral leishmaniasis (VL), and to compare PCR with conventional diagnostic techniques, like direct microscopy and parasite culture. Specimens of archived Giemsa-stained bone marrow slides from 91 patients with VL and from 79 controls with other diseases or conditions were studied. PCR showed the highest sensitivity (92.3%) and had good specificity (97.5%). Direct examination detected 79.1% and culture 59% of positive samples. In addition, PCR was able to detect VL in 16 of 19 patients (84.2%) with negative microscopy. PCR in Giemsa-stained bone marrow slides is a suitable tool for confirming diagnosis in patients with VL and may be useful in the diagnosis of difficult cases. Slide smears are easily stored, do not require special storage conditions such as low temperatures, and can be easily mailed to centers where PCR is available, making it an excellent option for diagnosis in the field.engMemórias do Instituto Oswaldo CruzLeishmaniose VisceralReação em Cadeia da PolimeraseDiagnósticoVisceral LeishmaniasisPolymerase Chain ReactionDiagnosisSensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in childreninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrustoloni, Yvone MaiaLima, Rosimar BatistaCunha, Rivaldo Venâncio daDorval, Maria ElizabethOshiro, Elisa TeruyaOliveira, Ana Lúcia Lyrio dePirmez, Claudeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSTHUMBNAILSensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children.pdf.jpgSensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children.pdf.jpgGenerated Thumbnailimage/jpeg1676https://repositorio.ufms.br/bitstream/123456789/1415/4/Sensitivity%20and%20specificity%20of%20polymerase%20chain%20reaction%20in%20Giemsa-stained%20slides%20for%20diagnosis%20of%20visceral%20leishmaniasis%20in%20children.pdf.jpg11100a3572f73553ab56568da8996133MD54ORIGINALSensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children.pdfSensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children.pdfapplication/pdf268541https://repositorio.ufms.br/bitstream/123456789/1415/1/Sensitivity%20and%20specificity%20of%20polymerase%20chain%20reaction%20in%20Giemsa-stained%20slides%20for%20diagnosis%20of%20visceral%20leishmaniasis%20in%20children.pdfa1bb41b9b9cdde9de4ae873f90249dbaMD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children |
title |
Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children |
spellingShingle |
Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children Brustoloni, Yvone Maia Leishmaniose Visceral Reação em Cadeia da Polimerase Diagnóstico Visceral Leishmaniasis Polymerase Chain Reaction Diagnosis |
title_short |
Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children |
title_full |
Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children |
title_fullStr |
Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children |
title_full_unstemmed |
Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children |
title_sort |
Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children |
author |
Brustoloni, Yvone Maia |
author_facet |
Brustoloni, Yvone Maia Lima, Rosimar Batista Cunha, Rivaldo Venâncio da Dorval, Maria Elizabeth Oshiro, Elisa Teruya Oliveira, Ana Lúcia Lyrio de Pirmez, Claude |
author_role |
author |
author2 |
Lima, Rosimar Batista Cunha, Rivaldo Venâncio da Dorval, Maria Elizabeth Oshiro, Elisa Teruya Oliveira, Ana Lúcia Lyrio de Pirmez, Claude |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Brustoloni, Yvone Maia Lima, Rosimar Batista Cunha, Rivaldo Venâncio da Dorval, Maria Elizabeth Oshiro, Elisa Teruya Oliveira, Ana Lúcia Lyrio de Pirmez, Claude |
dc.subject.por.fl_str_mv |
Leishmaniose Visceral Reação em Cadeia da Polimerase Diagnóstico Visceral Leishmaniasis Polymerase Chain Reaction Diagnosis |
topic |
Leishmaniose Visceral Reação em Cadeia da Polimerase Diagnóstico Visceral Leishmaniasis Polymerase Chain Reaction Diagnosis |
description |
The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in archived Giemsa-stained bone marrow slides for diagnosis of visceral leishmaniasis (VL), and to compare PCR with conventional diagnostic techniques, like direct microscopy and parasite culture. Specimens of archived Giemsa-stained bone marrow slides from 91 patients with VL and from 79 controls with other diseases or conditions were studied. PCR showed the highest sensitivity (92.3%) and had good specificity (97.5%). Direct examination detected 79.1% and culture 59% of positive samples. In addition, PCR was able to detect VL in 16 of 19 patients (84.2%) with negative microscopy. PCR in Giemsa-stained bone marrow slides is a suitable tool for confirming diagnosis in patients with VL and may be useful in the diagnosis of difficult cases. Slide smears are easily stored, do not require special storage conditions such as low temperatures, and can be easily mailed to centers where PCR is available, making it an excellent option for diagnosis in the field. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-05 |
dc.date.accessioned.fl_str_mv |
2012-05-22T18:50:27Z |
dc.date.available.fl_str_mv |
2021-09-30T19:55:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BRUSTOLONI, Yvone Maia et al . Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 102, n. 4, June 2007 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000400011&lng=en&nrm=iso>. access on 22 May 2012. Epub May 03, 2007. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufms.br/handle/123456789/1415 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762007005000036. |
identifier_str_mv |
BRUSTOLONI, Yvone Maia et al . Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 102, n. 4, June 2007 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000400011&lng=en&nrm=iso>. access on 22 May 2012. Epub May 03, 2007. |
url |
https://repositorio.ufms.br/handle/123456789/1415 http://dx.doi.org/10.1590/S0074-02762007005000036. |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz |
publisher.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMS instname:Universidade Federal de Mato Grosso do Sul (UFMS) instacron:UFMS |
instname_str |
Universidade Federal de Mato Grosso do Sul (UFMS) |
instacron_str |
UFMS |
institution |
UFMS |
reponame_str |
Repositório Institucional da UFMS |
collection |
Repositório Institucional da UFMS |
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