ATIVIDADE ANTIMICROBIANA, ANTIOXIDANTE E PROSPECÇÃO FITOQUÍMICA DOS EXTRATOS DE EQUISETUM PYRAMIDALE, POUTERIA RAMIFLORA, POROPHYLLUM RUDERALE, TAPIRIRA OBTUSA E SMILAX FLUMINENSIS.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMS |
Texto Completo: | https://repositorio.ufms.br/handle/123456789/4158 |
Resumo: | Infectious diseases, for decades, have been one of the most relevant public health problems. Antimicrobial resistance is a serious threat to treatment efficacy and responsible for high morbidity and mortality rates. There are bacteria resistant to all classes of antimicrobials available and the number of antifungal resistances is also on the rise. However, the production of new drugs by the pharmaceutical industry does not occur at the same speed. The objective of this work was to evaluate the antimicrobial and antioxidant activity, as well as the phytochemical composition of the extracts of Equisetum pyramidale, Pouteria ramiflora, Porophyllum ruderale, Tapirira obtusa and Smilax fluminensis extracts. Ethanol extracts from leaves and/or aerial parts of each botanical material were prepared. The classes of secondary metabolites were determined from a phytochemical screening. The contents of phenolic compounds and flavonoids were obtained by UV-visible. To determine the antimicrobial activity of extracts from these plants, susceptibility tests were performed using the disk diffusion method and microdilution in broth against resistant multidrug clinical isolates of Staphylococcus aureus and Klebsiella pneumoniae and against ATCC strains of Candida. From the extracts that inhibited microbial growth, the minimum bactericidal concentration and the minimum fungicidal concentration were determined. The total antioxidant activity of the extracts was determined by the DPPH free radical scavenging method (2.2-diphenyl-1-picrylhydrazyl). Phytochemical analysis showed the presence of some classes of secondary metabolites in common: phenolic compounds, flavonoids, steroids, tannins and saponins. The contents of phenolic compounds and flavonoids were significant, being higher in S. fluminensis (CF= 230,34 ± 1,20 mg/g (mg Catechin/g extract) e Flav.= 155,77 ± 0,99 (quercetin/g extract)), followed by E. pyramidale (CF= 205,4 ± 0,70 mg/g (mg Catechin/g extract) e Flav.= 150,9 ± 0,25 mg/g mg (quercetin/g extract)). The extract of S. fluminensis showed fungicidal activity on C. glabrata (ATCC 2001) (MIC=500 μg/mL). Bactericidal activity on MRSA (MIC=1000 μg/mL) was demonstrated by the extract of T. obtusa. All plant extracts tested showed antioxidant activity above 50% of DPPH free radical scavenging, the most significant being T. obtusa (82.36 ± 0.44 %) and S. fluminensis (69.89 ± 1.06 %). The S. fluminensis extract showed promising antifungal and antioxidant properties. Tapirira obtusa demonstrated antibacterial activity against a resistant pathogen and can be considered for further research, in addition to representing a potential natural antioxidant. Porophyllum ruderale, E. pyramidale, and P. ramiflora have antioxidant potential. Keywords: medicinal plants; antimicrobial activity; Klebsiella pneumonie; Staphylococcus aureus; Candida. |
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2021-12-01T22:01:21Z2021-12-01T22:01:21Z2021https://repositorio.ufms.br/handle/123456789/4158Infectious diseases, for decades, have been one of the most relevant public health problems. Antimicrobial resistance is a serious threat to treatment efficacy and responsible for high morbidity and mortality rates. There are bacteria resistant to all classes of antimicrobials available and the number of antifungal resistances is also on the rise. However, the production of new drugs by the pharmaceutical industry does not occur at the same speed. The objective of this work was to evaluate the antimicrobial and antioxidant activity, as well as the phytochemical composition of the extracts of Equisetum pyramidale, Pouteria ramiflora, Porophyllum ruderale, Tapirira obtusa and Smilax fluminensis extracts. Ethanol extracts from leaves and/or aerial parts of each botanical material were prepared. The classes of secondary metabolites were determined from a phytochemical screening. The contents of phenolic compounds and flavonoids were obtained by UV-visible. To determine the antimicrobial activity of extracts from these plants, susceptibility tests were performed using the disk diffusion method and microdilution in broth against resistant multidrug clinical isolates of Staphylococcus aureus and Klebsiella pneumoniae and against ATCC strains of Candida. From the extracts that inhibited microbial growth, the minimum bactericidal concentration and the minimum fungicidal concentration were determined. The total antioxidant activity of the extracts was determined by the DPPH free radical scavenging method (2.2-diphenyl-1-picrylhydrazyl). Phytochemical analysis showed the presence of some classes of secondary metabolites in common: phenolic compounds, flavonoids, steroids, tannins and saponins. The contents of phenolic compounds and flavonoids were significant, being higher in S. fluminensis (CF= 230,34 ± 1,20 mg/g (mg Catechin/g extract) e Flav.= 155,77 ± 0,99 (quercetin/g extract)), followed by E. pyramidale (CF= 205,4 ± 0,70 mg/g (mg Catechin/g extract) e Flav.= 150,9 ± 0,25 mg/g mg (quercetin/g extract)). The extract of S. fluminensis showed fungicidal activity on C. glabrata (ATCC 2001) (MIC=500 μg/mL). Bactericidal activity on MRSA (MIC=1000 μg/mL) was demonstrated by the extract of T. obtusa. All plant extracts tested showed antioxidant activity above 50% of DPPH free radical scavenging, the most significant being T. obtusa (82.36 ± 0.44 %) and S. fluminensis (69.89 ± 1.06 %). The S. fluminensis extract showed promising antifungal and antioxidant properties. Tapirira obtusa demonstrated antibacterial activity against a resistant pathogen and can be considered for further research, in addition to representing a potential natural antioxidant. Porophyllum ruderale, E. pyramidale, and P. ramiflora have antioxidant potential. Keywords: medicinal plants; antimicrobial activity; Klebsiella pneumonie; Staphylococcus aureus; Candida.As doenças infecciosas, por décadas, têm sido um dos problemas de saúde pública mais relevantes. A resistência antimicrobiana é uma séria ameaça à eficácia do tratamento e responsável por altos índices de morbimortalidade. Existem bactérias resistentes a todas as classes de antimicrobianos disponíveis e o número de resistência aos antifúngicos também está em ascensão. Entretanto, a produção de novos fármacos pela indústria farmacêutica não ocorre na mesma velocidade. O objetivo deste trabalho foi avaliar a atividade antimicrobiana e antioxidante, bem como a composição fitoquímica dos extratos de Equisetum pyramidale, Pouteria ramiflora, Porophyllum ruderale, Tapirira obtusa e Smilax fluminensis. Extratos etanólicos das folhas e/ou partes aéreas de cada material botânico foram preparados. As classes de metabólitos secundários foram determinadas a partir de um screening fitoquímico. Os teores de compostos fenólicos e flavonoides foram obtidos por UV-visível. Para determinar a atividade antimicrobiana dos extratos dessas plantas foram realizados testes de susceptibilidade pelo método de difusão em disco e microdiluição em caldo frente a isolados clínicos multidrogas resistentes de Staphylococcus aureus e Klebsiella pneumoniae e frente a cepas ATCC de Candida spp. Dos extratos que inibiram crescimento microbiano foram determinadas a concentração bactericida mínima e concentração fungicida mínima. A atividade antioxidante total dos extratos foi determinada pelo método de sequestro de radicais livres DPPH (2,2 difenil-1-picrilhidrazil). A análise fitoquímica demonstrou a presença de algumas classes de metabólitos secundários em comum: compostos fenólicos, flavonoides, esteroides, taninos e saponinas. Os teores de compostos fenólicos e flavonoides foram significativos, sendo superiores na S. fluminensis (CF= 230,34 ± 1,20 mg/g (mg Catequina/g extrato) e Flav.= 155,77 ± 0,99 (Quercetina/g extrato)), seguido da E. pyramidale (CF= 205,4 ± 0,70 mg/g (mg Catequina/g extrato) e Flav.= 150,9 ± 0,25 mg/g mg (Quercetina/g extrato)). O extrato de S. fluminensis apresentou atividade fungicida sobre C. glabrata (ATCC 2001) (CIM=500 μg/mL). Atividade bactericida sobre MRSA (CIM=1000 μg/mL) foi demonstrada pelo extrato de T. obtusa. Todos os extratos vegetais testados apresentaram atividade antioxidante acima de 50% de sequestro do radical livre DPPH, sendo que os mais significativos foram T. obtusa (82,36 ± 0,44 %) e S. fluminensis (69,89 ± 1,06). O extrato de S. fluminensis mostrou propriedades antifúngicas e antioxidantes promissoras. Tapirira obtusa demonstrou atividade antibacteriana sobre um patógeno resistente e pode ser considerada para novas pesquisas, além de representar um antioxidante natural em potencial. Porophyllum ruderale, E. pyramidale, e P. ramiflora possuem potencial antioxidante. Palavras-chave: plantas medicinais; atividade antimicrobiana; Klebsiella pneumoniae; Staphylococcus aureus; Candida.Fundação Universidade Federal de Mato Grosso do SulUFMSBrasilplantas medicinaisatividade antimicrobianaCandidaATIVIDADE ANTIMICROBIANA, ANTIOXIDANTE E PROSPECÇÃO FITOQUÍMICA DOS EXTRATOS DE EQUISETUM PYRAMIDALE, POUTERIA RAMIFLORA, POROPHYLLUM RUDERALE, TAPIRIRA OBTUSA E SMILAX FLUMINENSIS.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMarilene Rodrigues ChangDANIELE CRISTINA VITORELLI VENANCIOinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSTHUMBNAILDissertação Final_Daniele Venancio.pdf.jpgDissertação Final_Daniele Venancio.pdf.jpgGenerated Thumbnailimage/jpeg1221https://repositorio.ufms.br/bitstream/123456789/4158/3/Disserta%c3%a7%c3%a3o%20Final_Daniele%20Venancio.pdf.jpg1025212e6ce93a725eb742fdbfae62eeMD53TEXTDissertação Final_Daniele Venancio.pdf.txtDissertação Final_Daniele Venancio.pdf.txtExtracted texttext/plain134940https://repositorio.ufms.br/bitstream/123456789/4158/2/Disserta%c3%a7%c3%a3o%20Final_Daniele%20Venancio.pdf.txt762dce2ff7b450f3586d6eeb7b4ba1d7MD52ORIGINALDissertação Final_Daniele Venancio.pdfDissertação Final_Daniele Venancio.pdfapplication/pdf1294359https://repositorio.ufms.br/bitstream/123456789/4158/1/Disserta%c3%a7%c3%a3o%20Final_Daniele%20Venancio.pdf2cb8d470377504781a66a1bb352af4bbMD51123456789/41582021-12-02 03:01:43.828oai:repositorio.ufms.br:123456789/4158Repositório InstitucionalPUBhttps://repositorio.ufms.br/oai/requestri.prograd@ufms.bropendoar:21242021-12-02T07:01:43Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS)false |
dc.title.pt_BR.fl_str_mv |
ATIVIDADE ANTIMICROBIANA, ANTIOXIDANTE E PROSPECÇÃO FITOQUÍMICA DOS EXTRATOS DE EQUISETUM PYRAMIDALE, POUTERIA RAMIFLORA, POROPHYLLUM RUDERALE, TAPIRIRA OBTUSA E SMILAX FLUMINENSIS. |
title |
ATIVIDADE ANTIMICROBIANA, ANTIOXIDANTE E PROSPECÇÃO FITOQUÍMICA DOS EXTRATOS DE EQUISETUM PYRAMIDALE, POUTERIA RAMIFLORA, POROPHYLLUM RUDERALE, TAPIRIRA OBTUSA E SMILAX FLUMINENSIS. |
spellingShingle |
ATIVIDADE ANTIMICROBIANA, ANTIOXIDANTE E PROSPECÇÃO FITOQUÍMICA DOS EXTRATOS DE EQUISETUM PYRAMIDALE, POUTERIA RAMIFLORA, POROPHYLLUM RUDERALE, TAPIRIRA OBTUSA E SMILAX FLUMINENSIS. DANIELE CRISTINA VITORELLI VENANCIO plantas medicinais atividade antimicrobiana Candida |
title_short |
ATIVIDADE ANTIMICROBIANA, ANTIOXIDANTE E PROSPECÇÃO FITOQUÍMICA DOS EXTRATOS DE EQUISETUM PYRAMIDALE, POUTERIA RAMIFLORA, POROPHYLLUM RUDERALE, TAPIRIRA OBTUSA E SMILAX FLUMINENSIS. |
title_full |
ATIVIDADE ANTIMICROBIANA, ANTIOXIDANTE E PROSPECÇÃO FITOQUÍMICA DOS EXTRATOS DE EQUISETUM PYRAMIDALE, POUTERIA RAMIFLORA, POROPHYLLUM RUDERALE, TAPIRIRA OBTUSA E SMILAX FLUMINENSIS. |
title_fullStr |
ATIVIDADE ANTIMICROBIANA, ANTIOXIDANTE E PROSPECÇÃO FITOQUÍMICA DOS EXTRATOS DE EQUISETUM PYRAMIDALE, POUTERIA RAMIFLORA, POROPHYLLUM RUDERALE, TAPIRIRA OBTUSA E SMILAX FLUMINENSIS. |
title_full_unstemmed |
ATIVIDADE ANTIMICROBIANA, ANTIOXIDANTE E PROSPECÇÃO FITOQUÍMICA DOS EXTRATOS DE EQUISETUM PYRAMIDALE, POUTERIA RAMIFLORA, POROPHYLLUM RUDERALE, TAPIRIRA OBTUSA E SMILAX FLUMINENSIS. |
title_sort |
ATIVIDADE ANTIMICROBIANA, ANTIOXIDANTE E PROSPECÇÃO FITOQUÍMICA DOS EXTRATOS DE EQUISETUM PYRAMIDALE, POUTERIA RAMIFLORA, POROPHYLLUM RUDERALE, TAPIRIRA OBTUSA E SMILAX FLUMINENSIS. |
author |
DANIELE CRISTINA VITORELLI VENANCIO |
author_facet |
DANIELE CRISTINA VITORELLI VENANCIO |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Marilene Rodrigues Chang |
dc.contributor.author.fl_str_mv |
DANIELE CRISTINA VITORELLI VENANCIO |
contributor_str_mv |
Marilene Rodrigues Chang |
dc.subject.por.fl_str_mv |
plantas medicinais atividade antimicrobiana Candida |
topic |
plantas medicinais atividade antimicrobiana Candida |
description |
Infectious diseases, for decades, have been one of the most relevant public health problems. Antimicrobial resistance is a serious threat to treatment efficacy and responsible for high morbidity and mortality rates. There are bacteria resistant to all classes of antimicrobials available and the number of antifungal resistances is also on the rise. However, the production of new drugs by the pharmaceutical industry does not occur at the same speed. The objective of this work was to evaluate the antimicrobial and antioxidant activity, as well as the phytochemical composition of the extracts of Equisetum pyramidale, Pouteria ramiflora, Porophyllum ruderale, Tapirira obtusa and Smilax fluminensis extracts. Ethanol extracts from leaves and/or aerial parts of each botanical material were prepared. The classes of secondary metabolites were determined from a phytochemical screening. The contents of phenolic compounds and flavonoids were obtained by UV-visible. To determine the antimicrobial activity of extracts from these plants, susceptibility tests were performed using the disk diffusion method and microdilution in broth against resistant multidrug clinical isolates of Staphylococcus aureus and Klebsiella pneumoniae and against ATCC strains of Candida. From the extracts that inhibited microbial growth, the minimum bactericidal concentration and the minimum fungicidal concentration were determined. The total antioxidant activity of the extracts was determined by the DPPH free radical scavenging method (2.2-diphenyl-1-picrylhydrazyl). Phytochemical analysis showed the presence of some classes of secondary metabolites in common: phenolic compounds, flavonoids, steroids, tannins and saponins. The contents of phenolic compounds and flavonoids were significant, being higher in S. fluminensis (CF= 230,34 ± 1,20 mg/g (mg Catechin/g extract) e Flav.= 155,77 ± 0,99 (quercetin/g extract)), followed by E. pyramidale (CF= 205,4 ± 0,70 mg/g (mg Catechin/g extract) e Flav.= 150,9 ± 0,25 mg/g mg (quercetin/g extract)). The extract of S. fluminensis showed fungicidal activity on C. glabrata (ATCC 2001) (MIC=500 μg/mL). Bactericidal activity on MRSA (MIC=1000 μg/mL) was demonstrated by the extract of T. obtusa. All plant extracts tested showed antioxidant activity above 50% of DPPH free radical scavenging, the most significant being T. obtusa (82.36 ± 0.44 %) and S. fluminensis (69.89 ± 1.06 %). The S. fluminensis extract showed promising antifungal and antioxidant properties. Tapirira obtusa demonstrated antibacterial activity against a resistant pathogen and can be considered for further research, in addition to representing a potential natural antioxidant. Porophyllum ruderale, E. pyramidale, and P. ramiflora have antioxidant potential. Keywords: medicinal plants; antimicrobial activity; Klebsiella pneumonie; Staphylococcus aureus; Candida. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-12-01T22:01:21Z |
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2021-12-01T22:01:21Z |
dc.date.issued.fl_str_mv |
2021 |
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info:eu-repo/semantics/masterThesis |
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https://repositorio.ufms.br/handle/123456789/4158 |
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Fundação Universidade Federal de Mato Grosso do Sul |
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Brasil |
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