Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMS |
Texto Completo: | http://dx.doi.org/10.1590/0037-8682-0029-2013. https://repositorio.ufms.br/handle/123456789/1902 |
Resumo: | ABSTRACT - Introduction The progression of human papillomavirus (HPV) infection in the anogenital tract has been associated with the involvement of cells with regulatory properties. Evidence has shown that glucocorticoid-induced tumor necrosis factor receptor (GITR) is an important surface molecule for the characterization of these cells and proposes that GITR ligand may constitute a rational treatment for many cancer types. We aimed to detect the presence of GITR and CD25 in cervical stroma cells with and without pathological changes or HPV infection to better understand the immune response in the infected tissue microenvironment. Methods We subjected 49 paraffin-embedded cervical tissue samples to HPV DNA detection and histopathological analysis, and subsequently immunohistochemistry to detect GITR and CD25 in lymphocytes. Results We observed that 76.9% of all samples with high GITR expression were HPV-positive regardless of histopathological findings. High GITR expression (77.8%) was predominant in samples with ≥1,000 RLU/PCB. Of the HPV-positive samples negative for intraepithelial lesion and malignancy, 62.5% had high GITR expression. High GITR expression was observed in both carcinoma and high-grade squamous intraepithelial lesion (HSIL) samples (p = 0.16). CD25 was present in great quantities in all samples. Conclusions The predominance of high GITR expression in samples with high viral load that were classified as HSIL and carcinoma suggests that GITR+ cells can exhibit regulatory properties and may contribute to the progression of HPV-induced cervical neoplasia, emphasizing the importance of GITR as a potential target for immune therapy of cervical cancer and as a disease evolution biomarker. |
id |
UFMS_cf3f08c45ea6e75281e1c4830264b2cd |
---|---|
oai_identifier_str |
oai:repositorio.ufms.br:123456789/1902 |
network_acronym_str |
UFMS |
network_name_str |
Repositório Institucional da UFMS |
repository_id_str |
2124 |
spelling |
2014-02-15T00:47:16Z2021-09-30T19:57:06Z2013PADOVANI, Cacilda Tezelli Junqueira et al . Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection. Rev. Soc. Bras. Med. Trop., Uberaba, v. 46, n. 3, jun. 2013 . Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300288&lng=pt&nrm=iso>. acessos em 31 jul. 2013. http://dx.doi.org/10.1590/0037-8682-0029-2013.http://dx.doi.org/10.1590/0037-8682-0029-2013.https://repositorio.ufms.br/handle/123456789/1902ABSTRACT - Introduction The progression of human papillomavirus (HPV) infection in the anogenital tract has been associated with the involvement of cells with regulatory properties. Evidence has shown that glucocorticoid-induced tumor necrosis factor receptor (GITR) is an important surface molecule for the characterization of these cells and proposes that GITR ligand may constitute a rational treatment for many cancer types. We aimed to detect the presence of GITR and CD25 in cervical stroma cells with and without pathological changes or HPV infection to better understand the immune response in the infected tissue microenvironment. Methods We subjected 49 paraffin-embedded cervical tissue samples to HPV DNA detection and histopathological analysis, and subsequently immunohistochemistry to detect GITR and CD25 in lymphocytes. Results We observed that 76.9% of all samples with high GITR expression were HPV-positive regardless of histopathological findings. High GITR expression (77.8%) was predominant in samples with ≥1,000 RLU/PCB. Of the HPV-positive samples negative for intraepithelial lesion and malignancy, 62.5% had high GITR expression. High GITR expression was observed in both carcinoma and high-grade squamous intraepithelial lesion (HSIL) samples (p = 0.16). CD25 was present in great quantities in all samples. Conclusions The predominance of high GITR expression in samples with high viral load that were classified as HSIL and carcinoma suggests that GITR+ cells can exhibit regulatory properties and may contribute to the progression of HPV-induced cervical neoplasia, emphasizing the importance of GITR as a potential target for immune therapy of cervical cancer and as a disease evolution biomarker.engRevista da Sociedade Brasileira de Medicina TropicalPapillomavirus Humano 16Papillomavirus Humano 18Papillomavirus Humano 31ImunoistoquímicaImunidadeHuman papillomavirus 16Human papillomavirus 18Human papillomavirus 31ImmunohistochemistryImmunityGlucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePadovan, Cacilda Tezelli JunqueiraBonin, Camila MaretiTozetti, Inês AparecidaFerreira, Alda Maria TeixeiraFernandes, Carlos Eurico dos SantosCosta, Izaias Pereira dainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSTHUMBNAILGlucocorticoid-induced tumor.pdf.jpgGlucocorticoid-induced tumor.pdf.jpgGenerated Thumbnailimage/jpeg1798https://repositorio.ufms.br/bitstream/123456789/1902/4/Glucocorticoid-induced%20tumor.pdf.jpg8658b82c0fc39c8bd427bfb1dd7bf1bcMD54ORIGINALGlucocorticoid-induced tumor.pdfGlucocorticoid-induced tumor.pdfapplication/pdf2689276https://repositorio.ufms.br/bitstream/123456789/1902/1/Glucocorticoid-induced%20tumor.pdfd41d1651b2d8380cae00020b5145ae82MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufms.br/bitstream/123456789/1902/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTGlucocorticoid-induced tumor.pdf.txtGlucocorticoid-induced tumor.pdf.txtExtracted texttext/plain27421https://repositorio.ufms.br/bitstream/123456789/1902/3/Glucocorticoid-induced%20tumor.pdf.txt70346c7296653c9bbc3bc616c746e917MD53123456789/19022021-09-30 15:57:06.543oai:repositorio.ufms.br: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Repositório InstitucionalPUBhttps://repositorio.ufms.br/oai/requestri.prograd@ufms.bropendoar:21242021-09-30T19:57:06Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS)false |
dc.title.pt_BR.fl_str_mv |
Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection |
title |
Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection |
spellingShingle |
Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection Padovan, Cacilda Tezelli Junqueira Papillomavirus Humano 16 Papillomavirus Humano 18 Papillomavirus Humano 31 Imunoistoquímica Imunidade Human papillomavirus 16 Human papillomavirus 18 Human papillomavirus 31 Immunohistochemistry Immunity |
title_short |
Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection |
title_full |
Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection |
title_fullStr |
Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection |
title_full_unstemmed |
Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection |
title_sort |
Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection |
author |
Padovan, Cacilda Tezelli Junqueira |
author_facet |
Padovan, Cacilda Tezelli Junqueira Bonin, Camila Mareti Tozetti, Inês Aparecida Ferreira, Alda Maria Teixeira Fernandes, Carlos Eurico dos Santos Costa, Izaias Pereira da |
author_role |
author |
author2 |
Bonin, Camila Mareti Tozetti, Inês Aparecida Ferreira, Alda Maria Teixeira Fernandes, Carlos Eurico dos Santos Costa, Izaias Pereira da |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Padovan, Cacilda Tezelli Junqueira Bonin, Camila Mareti Tozetti, Inês Aparecida Ferreira, Alda Maria Teixeira Fernandes, Carlos Eurico dos Santos Costa, Izaias Pereira da |
dc.subject.por.fl_str_mv |
Papillomavirus Humano 16 Papillomavirus Humano 18 Papillomavirus Humano 31 Imunoistoquímica Imunidade Human papillomavirus 16 Human papillomavirus 18 Human papillomavirus 31 Immunohistochemistry Immunity |
topic |
Papillomavirus Humano 16 Papillomavirus Humano 18 Papillomavirus Humano 31 Imunoistoquímica Imunidade Human papillomavirus 16 Human papillomavirus 18 Human papillomavirus 31 Immunohistochemistry Immunity |
description |
ABSTRACT - Introduction The progression of human papillomavirus (HPV) infection in the anogenital tract has been associated with the involvement of cells with regulatory properties. Evidence has shown that glucocorticoid-induced tumor necrosis factor receptor (GITR) is an important surface molecule for the characterization of these cells and proposes that GITR ligand may constitute a rational treatment for many cancer types. We aimed to detect the presence of GITR and CD25 in cervical stroma cells with and without pathological changes or HPV infection to better understand the immune response in the infected tissue microenvironment. Methods We subjected 49 paraffin-embedded cervical tissue samples to HPV DNA detection and histopathological analysis, and subsequently immunohistochemistry to detect GITR and CD25 in lymphocytes. Results We observed that 76.9% of all samples with high GITR expression were HPV-positive regardless of histopathological findings. High GITR expression (77.8%) was predominant in samples with ≥1,000 RLU/PCB. Of the HPV-positive samples negative for intraepithelial lesion and malignancy, 62.5% had high GITR expression. High GITR expression was observed in both carcinoma and high-grade squamous intraepithelial lesion (HSIL) samples (p = 0.16). CD25 was present in great quantities in all samples. Conclusions The predominance of high GITR expression in samples with high viral load that were classified as HSIL and carcinoma suggests that GITR+ cells can exhibit regulatory properties and may contribute to the progression of HPV-induced cervical neoplasia, emphasizing the importance of GITR as a potential target for immune therapy of cervical cancer and as a disease evolution biomarker. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2014-02-15T00:47:16Z |
dc.date.available.fl_str_mv |
2021-09-30T19:57:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PADOVANI, Cacilda Tezelli Junqueira et al . Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection. Rev. Soc. Bras. Med. Trop., Uberaba, v. 46, n. 3, jun. 2013 . Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300288&lng=pt&nrm=iso>. acessos em 31 jul. 2013. http://dx.doi.org/10.1590/0037-8682-0029-2013. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufms.br/handle/123456789/1902 |
dc.identifier.issn.none.fl_str_mv |
http://dx.doi.org/10.1590/0037-8682-0029-2013. |
identifier_str_mv |
PADOVANI, Cacilda Tezelli Junqueira et al . Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection. Rev. Soc. Bras. Med. Trop., Uberaba, v. 46, n. 3, jun. 2013 . Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300288&lng=pt&nrm=iso>. acessos em 31 jul. 2013. http://dx.doi.org/10.1590/0037-8682-0029-2013. |
url |
http://dx.doi.org/10.1590/0037-8682-0029-2013. https://repositorio.ufms.br/handle/123456789/1902 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical |
publisher.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMS instname:Universidade Federal de Mato Grosso do Sul (UFMS) instacron:UFMS |
instname_str |
Universidade Federal de Mato Grosso do Sul (UFMS) |
instacron_str |
UFMS |
institution |
UFMS |
reponame_str |
Repositório Institucional da UFMS |
collection |
Repositório Institucional da UFMS |
bitstream.url.fl_str_mv |
https://repositorio.ufms.br/bitstream/123456789/1902/4/Glucocorticoid-induced%20tumor.pdf.jpg https://repositorio.ufms.br/bitstream/123456789/1902/1/Glucocorticoid-induced%20tumor.pdf https://repositorio.ufms.br/bitstream/123456789/1902/2/license.txt https://repositorio.ufms.br/bitstream/123456789/1902/3/Glucocorticoid-induced%20tumor.pdf.txt |
bitstream.checksum.fl_str_mv |
8658b82c0fc39c8bd427bfb1dd7bf1bc d41d1651b2d8380cae00020b5145ae82 8a4605be74aa9ea9d79846c1fba20a33 70346c7296653c9bbc3bc616c746e917 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS) |
repository.mail.fl_str_mv |
ri.prograd@ufms.br |
_version_ |
1815448038827622400 |