Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection

Detalhes bibliográficos
Autor(a) principal: Padovan, Cacilda Tezelli Junqueira
Data de Publicação: 2013
Outros Autores: Bonin, Camila Mareti, Tozetti, Inês Aparecida, Ferreira, Alda Maria Teixeira, Fernandes, Carlos Eurico dos Santos, Costa, Izaias Pereira da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMS
Texto Completo: http://dx.doi.org/10.1590/0037-8682-0029-2013.
https://repositorio.ufms.br/handle/123456789/1902
Resumo: ABSTRACT - Introduction The progression of human papillomavirus (HPV) infection in the anogenital tract has been associated with the involvement of cells with regulatory properties. Evidence has shown that glucocorticoid-induced tumor necrosis factor receptor (GITR) is an important surface molecule for the characterization of these cells and proposes that GITR ligand may constitute a rational treatment for many cancer types. We aimed to detect the presence of GITR and CD25 in cervical stroma cells with and without pathological changes or HPV infection to better understand the immune response in the infected tissue microenvironment. Methods We subjected 49 paraffin-embedded cervical tissue samples to HPV DNA detection and histopathological analysis, and subsequently immunohistochemistry to detect GITR and CD25 in lymphocytes. Results We observed that 76.9% of all samples with high GITR expression were HPV-positive regardless of histopathological findings. High GITR expression (77.8%) was predominant in samples with ≥1,000 RLU/PCB. Of the HPV-positive samples negative for intraepithelial lesion and malignancy, 62.5% had high GITR expression. High GITR expression was observed in both carcinoma and high-grade squamous intraepithelial lesion (HSIL) samples (p = 0.16). CD25 was present in great quantities in all samples. Conclusions The predominance of high GITR expression in samples with high viral load that were classified as HSIL and carcinoma suggests that GITR+ cells can exhibit regulatory properties and may contribute to the progression of HPV-induced cervical neoplasia, emphasizing the importance of GITR as a potential target for immune therapy of cervical cancer and as a disease evolution biomarker.
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spelling 2014-02-15T00:47:16Z2021-09-30T19:57:06Z2013PADOVANI, Cacilda Tezelli Junqueira et al . Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection. Rev. Soc. Bras. Med. Trop., Uberaba, v. 46, n. 3, jun. 2013 . Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300288&lng=pt&nrm=iso>. acessos em 31 jul. 2013. http://dx.doi.org/10.1590/0037-8682-0029-2013.http://dx.doi.org/10.1590/0037-8682-0029-2013.https://repositorio.ufms.br/handle/123456789/1902ABSTRACT - Introduction The progression of human papillomavirus (HPV) infection in the anogenital tract has been associated with the involvement of cells with regulatory properties. Evidence has shown that glucocorticoid-induced tumor necrosis factor receptor (GITR) is an important surface molecule for the characterization of these cells and proposes that GITR ligand may constitute a rational treatment for many cancer types. We aimed to detect the presence of GITR and CD25 in cervical stroma cells with and without pathological changes or HPV infection to better understand the immune response in the infected tissue microenvironment. Methods We subjected 49 paraffin-embedded cervical tissue samples to HPV DNA detection and histopathological analysis, and subsequently immunohistochemistry to detect GITR and CD25 in lymphocytes. Results We observed that 76.9% of all samples with high GITR expression were HPV-positive regardless of histopathological findings. High GITR expression (77.8%) was predominant in samples with ≥1,000 RLU/PCB. Of the HPV-positive samples negative for intraepithelial lesion and malignancy, 62.5% had high GITR expression. High GITR expression was observed in both carcinoma and high-grade squamous intraepithelial lesion (HSIL) samples (p = 0.16). CD25 was present in great quantities in all samples. Conclusions The predominance of high GITR expression in samples with high viral load that were classified as HSIL and carcinoma suggests that GITR+ cells can exhibit regulatory properties and may contribute to the progression of HPV-induced cervical neoplasia, emphasizing the importance of GITR as a potential target for immune therapy of cervical cancer and as a disease evolution biomarker.engRevista da Sociedade Brasileira de Medicina TropicalPapillomavirus Humano 16Papillomavirus Humano 18Papillomavirus Humano 31ImunoistoquímicaImunidadeHuman papillomavirus 16Human papillomavirus 18Human papillomavirus 31ImmunohistochemistryImmunityGlucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePadovan, Cacilda Tezelli JunqueiraBonin, Camila MaretiTozetti, Inês AparecidaFerreira, Alda Maria TeixeiraFernandes, Carlos Eurico dos SantosCosta, Izaias Pereira dainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSTHUMBNAILGlucocorticoid-induced tumor.pdf.jpgGlucocorticoid-induced tumor.pdf.jpgGenerated Thumbnailimage/jpeg1798https://repositorio.ufms.br/bitstream/123456789/1902/4/Glucocorticoid-induced%20tumor.pdf.jpg8658b82c0fc39c8bd427bfb1dd7bf1bcMD54ORIGINALGlucocorticoid-induced tumor.pdfGlucocorticoid-induced tumor.pdfapplication/pdf2689276https://repositorio.ufms.br/bitstream/123456789/1902/1/Glucocorticoid-induced%20tumor.pdfd41d1651b2d8380cae00020b5145ae82MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufms.br/bitstream/123456789/1902/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTGlucocorticoid-induced tumor.pdf.txtGlucocorticoid-induced tumor.pdf.txtExtracted texttext/plain27421https://repositorio.ufms.br/bitstream/123456789/1902/3/Glucocorticoid-induced%20tumor.pdf.txt70346c7296653c9bbc3bc616c746e917MD53123456789/19022021-09-30 15:57:06.543oai:repositorio.ufms.br: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Repositório InstitucionalPUBhttps://repositorio.ufms.br/oai/requestri.prograd@ufms.bropendoar:21242021-09-30T19:57:06Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS)false
dc.title.pt_BR.fl_str_mv Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection
title Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection
spellingShingle Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection
Padovan, Cacilda Tezelli Junqueira
Papillomavirus Humano 16
Papillomavirus Humano 18
Papillomavirus Humano 31
Imunoistoquímica
Imunidade
Human papillomavirus 16
Human papillomavirus 18
Human papillomavirus 31
Immunohistochemistry
Immunity
title_short Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection
title_full Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection
title_fullStr Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection
title_full_unstemmed Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection
title_sort Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection
author Padovan, Cacilda Tezelli Junqueira
author_facet Padovan, Cacilda Tezelli Junqueira
Bonin, Camila Mareti
Tozetti, Inês Aparecida
Ferreira, Alda Maria Teixeira
Fernandes, Carlos Eurico dos Santos
Costa, Izaias Pereira da
author_role author
author2 Bonin, Camila Mareti
Tozetti, Inês Aparecida
Ferreira, Alda Maria Teixeira
Fernandes, Carlos Eurico dos Santos
Costa, Izaias Pereira da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Padovan, Cacilda Tezelli Junqueira
Bonin, Camila Mareti
Tozetti, Inês Aparecida
Ferreira, Alda Maria Teixeira
Fernandes, Carlos Eurico dos Santos
Costa, Izaias Pereira da
dc.subject.por.fl_str_mv Papillomavirus Humano 16
Papillomavirus Humano 18
Papillomavirus Humano 31
Imunoistoquímica
Imunidade
Human papillomavirus 16
Human papillomavirus 18
Human papillomavirus 31
Immunohistochemistry
Immunity
topic Papillomavirus Humano 16
Papillomavirus Humano 18
Papillomavirus Humano 31
Imunoistoquímica
Imunidade
Human papillomavirus 16
Human papillomavirus 18
Human papillomavirus 31
Immunohistochemistry
Immunity
description ABSTRACT - Introduction The progression of human papillomavirus (HPV) infection in the anogenital tract has been associated with the involvement of cells with regulatory properties. Evidence has shown that glucocorticoid-induced tumor necrosis factor receptor (GITR) is an important surface molecule for the characterization of these cells and proposes that GITR ligand may constitute a rational treatment for many cancer types. We aimed to detect the presence of GITR and CD25 in cervical stroma cells with and without pathological changes or HPV infection to better understand the immune response in the infected tissue microenvironment. Methods We subjected 49 paraffin-embedded cervical tissue samples to HPV DNA detection and histopathological analysis, and subsequently immunohistochemistry to detect GITR and CD25 in lymphocytes. Results We observed that 76.9% of all samples with high GITR expression were HPV-positive regardless of histopathological findings. High GITR expression (77.8%) was predominant in samples with ≥1,000 RLU/PCB. Of the HPV-positive samples negative for intraepithelial lesion and malignancy, 62.5% had high GITR expression. High GITR expression was observed in both carcinoma and high-grade squamous intraepithelial lesion (HSIL) samples (p = 0.16). CD25 was present in great quantities in all samples. Conclusions The predominance of high GITR expression in samples with high viral load that were classified as HSIL and carcinoma suggests that GITR+ cells can exhibit regulatory properties and may contribute to the progression of HPV-induced cervical neoplasia, emphasizing the importance of GITR as a potential target for immune therapy of cervical cancer and as a disease evolution biomarker.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-02-15T00:47:16Z
dc.date.available.fl_str_mv 2021-09-30T19:57:06Z
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dc.identifier.citation.fl_str_mv PADOVANI, Cacilda Tezelli Junqueira et al . Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection. Rev. Soc. Bras. Med. Trop., Uberaba, v. 46, n. 3, jun. 2013 . Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300288&lng=pt&nrm=iso>. acessos em 31 jul. 2013. http://dx.doi.org/10.1590/0037-8682-0029-2013.
dc.identifier.uri.fl_str_mv https://repositorio.ufms.br/handle/123456789/1902
dc.identifier.issn.none.fl_str_mv http://dx.doi.org/10.1590/0037-8682-0029-2013.
identifier_str_mv PADOVANI, Cacilda Tezelli Junqueira et al . Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection. Rev. Soc. Bras. Med. Trop., Uberaba, v. 46, n. 3, jun. 2013 . Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300288&lng=pt&nrm=iso>. acessos em 31 jul. 2013. http://dx.doi.org/10.1590/0037-8682-0029-2013.
url http://dx.doi.org/10.1590/0037-8682-0029-2013.
https://repositorio.ufms.br/handle/123456789/1902
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dc.publisher.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical
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