Análise da variabilidade genética de duas populações de murici da espécie Byrsonima cydoniifolia A.Juss. através de marcadores microssatélite

• Autor(a) principal: Rodrigues, Kemilly Vaz
• Data de Publicação: 2021
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Biblioteca Digital de Monografias da UFMT
• Texto Completo: http://bdm.ufmt.br/handle/1/2354
• topic: CNPQ::CIENCIAS AGRARIAS::AGRONOMIA; banco de germoplasma; Cerrado; frutífera nativa; marcadores moleculares; variabilidade genética; germplasm bank; cerrado; native fruit; molecular markers; genetic variabily

Murici (Byrsonima cydoniifolia A. Juss.) Is a small shrub in the Cerrado biome that has potential in folk medicine, food and firewood production. It is a species considered nondomesticated and, given its economic and ecological potential, basic studies on its genetic diversity must be carried out. This study aimed to carry out the analysis of genetic variability in two populations of murici of the species Byrsonima cydoniifolia A.Juss. existing in the Germplasm Bank of the Federal University of Mato Grosso, campus of Araguaia, through microsatellite markers. Samples were collected from 96 individuals from two populations called Beija-Flor (N = 52) and Alencar (N = 44) from the germplasm bank existing at the Federal University of mato Grosso, campus of Araguaia. DNA extraction, quantification and amplification were performed. The DNA was subjected to microsatellite molecular marker techniques and subsequently performed statistical analysis to estimate the allelic frequencies, heterozygosity and the fixation index (Fis). Eight microsatellite loci were used, which enabled the genetic discrimination of the 96 individuals. The amplified microsatellite regions revealed polymorphism in the two populations studied, which may be useful for the study of genetic diversity in this species. Greater heterozygosity was observed in the Alencar population than in Beija-Flor, both for allelic frequency analyzes and for Fis, being sufficient for the initial breeding programs. The results of Fis obtained allowed to say that the reproduction of the species is mixed, where Alencar had 86.1% of crossed fertilization and Beija-Flor, 79.3% of crossed fertilization.