Influência do tempo e temperatura na conservação de proteínas totais, albumina, globulinas em amostras de cães

• Autor(a) principal: Palermo, Ana Leticia Prata
• Data de Publicação: 2022
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Biblioteca Digital de Monografias da UFMT
• Texto Completo: http://bdm.ufmt.br/handle/1/3906
• topic: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA; Armazenamento; Soro sanguíneo; Proteínas séricas; Soro canino; Storage; Blood serum; Serum proteins; Canine serum

ABSTRACT: Serum proteins play important roles in the organism of living beings and are exclusively produced endogenously. Changes such as hypoproteinemia and hyperproteinemia may reflect renal, hepatic, gastrointestinal and other body systems disorders. The measurement of serum protein concentration is extremely important for the diagnosis and prognosis of diseases, however, it often cannot be done in fresh serum, having to travel long distances, spending time, to reference laboratories. Studies on the effect of storage temperature and storage time on the concentration dosages of these proteins. In this study, blood samples were collected from 50 dogs and stored under refrigeration for 6, 24, 36, 72 and 96 hours and frozen for 7, 15, 30, 60, 90 and 120 days to evaluate the stability in the concentration of total proteins, albumin , globulins and albumin/globulin index (A/G) using fresh serum as a control. According to the statistical analyzes used, there were no significant changes (p <0.05) in the concentrations, thus concluding that, under refrigeration for up to 96 hours and freezing for up to 120 days, the samples are viable for measuring total proteins, albumin, globulins and in the index A/G.