Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Monografias da UFMT |
Texto Completo: | http://bdm.ufmt.br/handle/1/1775 |
Resumo: | Endophytic fungi are microorganisms that inhabit plant tissues and are recognized for their potential in the production of important products of biotechnological interest. They can be considered important sources of enzymes of commercial interest, such as amylase, which acts on the degradation of starch, and asparaginase of great importance in the medical / pharmacological area for the control / treatment of tumor cells. In this context, the work aimed to prospect for the enzyme asparaginase and amylase produced by endophytic fungi isolated from the medicinal plant Moringa oleifera. For the screening of asparaginase, a specific medium containing L-asparagine (10g / L) was used as a substrate, in addition to glucose, KH2PO4 (1.52g / L), KCl (0.52g / L), MgSO4 .7H2O ( 0.26g / L), CuNO3 .3H2O (0.001g / L), ZnSO4 .7H2O (0.001g / L), FeSO4 .7H2O (0.001g / L), agar (20g / L) and the red phenol indicator ( 0.009%) with pH 6.8 and 8.2 turning range. The formation of a red zone around the colony indicated the production capacity of L-asparaginase by the fungus. For the screening of amylase-producing fungi, a specific medium containing NaCl (0.26g / L), yeast extract (0.4g / L), KH2PO4 (0.5g / L), MgSO4.7H2O (0 , 2g / L), Agar (15g / L) plus 0.2% soluble starch. After 96 hours of incubation at room temperature, the plates were developed with KI (1%). The enzymatic activity was evaluated through the halo formed which indicates the degradation of the substrate. The results for both enzymes were expressed using an enzyme index. For the screening of asparaginase, 36 endophytes were analyzed, where 1 did not show enzyme activity (2.77%), 3 (8.33%) showed weak activity, 26 (72.22%) moderate and 6 (16.68%) high asparaginase activity. To prospect for amylase, 26 endophytic fungi were studied, with 96% showing intermediate activity and 4% high amylolytic activity. The results were promising, demonstrating the production of the enzymes amylase and asparaginase by a large percentage of the isolated fungi, although at different levels, it can be considered a niche of microorganisms with great biotechnological potential to be explored. |
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Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilaseCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALFungos endofiticosAsparaginaseAmilaseMoringa oleiferaEndophytic fungiAsparaginaseAmylaseMoringa oleiferaEndophytic fungi are microorganisms that inhabit plant tissues and are recognized for their potential in the production of important products of biotechnological interest. They can be considered important sources of enzymes of commercial interest, such as amylase, which acts on the degradation of starch, and asparaginase of great importance in the medical / pharmacological area for the control / treatment of tumor cells. In this context, the work aimed to prospect for the enzyme asparaginase and amylase produced by endophytic fungi isolated from the medicinal plant Moringa oleifera. For the screening of asparaginase, a specific medium containing L-asparagine (10g / L) was used as a substrate, in addition to glucose, KH2PO4 (1.52g / L), KCl (0.52g / L), MgSO4 .7H2O ( 0.26g / L), CuNO3 .3H2O (0.001g / L), ZnSO4 .7H2O (0.001g / L), FeSO4 .7H2O (0.001g / L), agar (20g / L) and the red phenol indicator ( 0.009%) with pH 6.8 and 8.2 turning range. The formation of a red zone around the colony indicated the production capacity of L-asparaginase by the fungus. For the screening of amylase-producing fungi, a specific medium containing NaCl (0.26g / L), yeast extract (0.4g / L), KH2PO4 (0.5g / L), MgSO4.7H2O (0 , 2g / L), Agar (15g / L) plus 0.2% soluble starch. After 96 hours of incubation at room temperature, the plates were developed with KI (1%). The enzymatic activity was evaluated through the halo formed which indicates the degradation of the substrate. The results for both enzymes were expressed using an enzyme index. For the screening of asparaginase, 36 endophytes were analyzed, where 1 did not show enzyme activity (2.77%), 3 (8.33%) showed weak activity, 26 (72.22%) moderate and 6 (16.68%) high asparaginase activity. To prospect for amylase, 26 endophytic fungi were studied, with 96% showing intermediate activity and 4% high amylolytic activity. The results were promising, demonstrating the production of the enzymes amylase and asparaginase by a large percentage of the isolated fungi, although at different levels, it can be considered a niche of microorganisms with great biotechnological potential to be explored.Fungos endofíticos são microrganismos que habitam os tecidos das plantas e são reconhecidos por seu potencial na produção de importantes produtos de interesse biotecnológico. Podem ser considerados importantes fontes de enzimas de interesse comercial, como a amilase, que atua na degradação do amido, e asparaginase de grande importância na área médica/farmacológica para o controle/tratamento de células tumorais. Neste contexto, o trabalho teve como objetivo realizar a prospecção da enzima asparaginase e amilase produzidas por fungos endofíticos isolados da planta medicinal Moringa oleifera. Para a triagem da asparaginase utilizou-se um meio especifico contendo contendo L-asparagina(10g/L) como substrato, além de glicose, KH2PO4 (1,52g/L), KCl (0,52g/L), MgSO4 .7H2O(0,26g/L), CuNO3 .3H2O(0,001g/L), ZnSO4 .7H2O(0,001g/L), FeSO4 .7H2O(0,001g/L), ágar (20g/L) e o indicador vermelho de fenol (0,009%) com faixa de viragem pH 6,8 e 8,2. A formação de uma zona vermelha ao redor da colônia indicou a capacidade de produção de Lasparaginase pelo fungo. Para a triagem de fungos produtores de amilase, utilizou-se um meio especifico contendo NaCl(0,26g/L), extrato de levedura (0,4g/L), KH2PO4 (0,5g/L), MgSO4.7H2O(0,2g/L), Ágar(15g/L) acrescido 0,2% de amido solúvel. Após 96 horas de incubação à temperatura ambiente, as placas foram reveladas com KI (1%). A atividade enzimática foi avaliada através do halo formado o qual indica a degradação do substrato. Os resultados para as duas enzimas foram expressos através de índice enzimático. Para a triagem de asparaginase foram analisados 36 endófitos onde, 1 não apresentou atividade enzimática (2,77%), 3 (8,33%) mostraram fraca atividade, 26 (72,22%) moderada e 6 (16,68%) alta atividade asparaginase. Para prospecção de amilase, 26 fungos endofíticos foram estudados, com 96% apresentando atividade intermediária e 4 % alta atividade amilolítica. Os Resultados foram promissores, demonstrando a produção das enzimas amilase e asparaginase por grande percentual dos fungos isolados, ainda que em diferentes níveis podendo ser considerado um nicho de micro-organismos de grande potencial biotecnológico a ser explorado.Universidade Federal de Mato GrossoBrasilInstituto de Ciências Exatas e Naturais (ICEN) – RondonópolisUFMT CUR - RondonopólisCiências Biológicas - CURLisboa, Helen Cristina Fáverohttp://lattes.cnpq.br/5820048364853772Lisboa, Helen Cristina Faverohttp://lattes.cnpq.br/5820048364853772Teles, Helder Lopeshttp://lattes.cnpq.br/5902911518497333Silva, Virginia Siqueira dahttp://lattes.cnpq.br/4679073761905374Santos, Luís Felipe Oliva dos2020-10-27T20:03:14Z2020-03-042020-10-27T20:03:14Z2020-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/datasetSANTOS, Luís Felipe Oliva dos. Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase. 2019. 36 f. Trabalho de Conclusão de Curso (Graduação em Ciências Biológicas) – Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e Biológicas, Rondonópolis, 2019.http://bdm.ufmt.br/handle/1/1775porinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Monografias da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2020-11-06T06:00:28Zoai:localhost:1/1775Biblioteca Digital de Monografiahttps://bdm.ufmt.br/PUBhttp://200.129.241.122/oai/requestopendoar:2020-11-06T06:00:28falseBiblioteca Digital de Monografiahttps://bdm.ufmt.br/PUBhttp://200.129.241.122/oai/requestbibliotecacentral@ufmt.br||opendoar:2020-11-06T06:00:28Biblioteca Digital de Monografias da UFMT - Universidade Federal de Mato Grosso (UFMT)false |
dc.title.none.fl_str_mv |
Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase |
title |
Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase |
spellingShingle |
Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase Santos, Luís Felipe Oliva dos CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL Fungos endofiticos Asparaginase Amilase Moringa oleifera Endophytic fungi Asparaginase Amylase Moringa oleifera |
title_short |
Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase |
title_full |
Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase |
title_fullStr |
Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase |
title_full_unstemmed |
Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase |
title_sort |
Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase |
author |
Santos, Luís Felipe Oliva dos |
author_facet |
Santos, Luís Felipe Oliva dos |
author_role |
author |
dc.contributor.none.fl_str_mv |
Lisboa, Helen Cristina Fávero http://lattes.cnpq.br/5820048364853772 Lisboa, Helen Cristina Favero http://lattes.cnpq.br/5820048364853772 Teles, Helder Lopes http://lattes.cnpq.br/5902911518497333 Silva, Virginia Siqueira da http://lattes.cnpq.br/4679073761905374 |
dc.contributor.author.fl_str_mv |
Santos, Luís Felipe Oliva dos |
dc.subject.por.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL Fungos endofiticos Asparaginase Amilase Moringa oleifera Endophytic fungi Asparaginase Amylase Moringa oleifera |
topic |
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL Fungos endofiticos Asparaginase Amilase Moringa oleifera Endophytic fungi Asparaginase Amylase Moringa oleifera |
description |
Endophytic fungi are microorganisms that inhabit plant tissues and are recognized for their potential in the production of important products of biotechnological interest. They can be considered important sources of enzymes of commercial interest, such as amylase, which acts on the degradation of starch, and asparaginase of great importance in the medical / pharmacological area for the control / treatment of tumor cells. In this context, the work aimed to prospect for the enzyme asparaginase and amylase produced by endophytic fungi isolated from the medicinal plant Moringa oleifera. For the screening of asparaginase, a specific medium containing L-asparagine (10g / L) was used as a substrate, in addition to glucose, KH2PO4 (1.52g / L), KCl (0.52g / L), MgSO4 .7H2O ( 0.26g / L), CuNO3 .3H2O (0.001g / L), ZnSO4 .7H2O (0.001g / L), FeSO4 .7H2O (0.001g / L), agar (20g / L) and the red phenol indicator ( 0.009%) with pH 6.8 and 8.2 turning range. The formation of a red zone around the colony indicated the production capacity of L-asparaginase by the fungus. For the screening of amylase-producing fungi, a specific medium containing NaCl (0.26g / L), yeast extract (0.4g / L), KH2PO4 (0.5g / L), MgSO4.7H2O (0 , 2g / L), Agar (15g / L) plus 0.2% soluble starch. After 96 hours of incubation at room temperature, the plates were developed with KI (1%). The enzymatic activity was evaluated through the halo formed which indicates the degradation of the substrate. The results for both enzymes were expressed using an enzyme index. For the screening of asparaginase, 36 endophytes were analyzed, where 1 did not show enzyme activity (2.77%), 3 (8.33%) showed weak activity, 26 (72.22%) moderate and 6 (16.68%) high asparaginase activity. To prospect for amylase, 26 endophytic fungi were studied, with 96% showing intermediate activity and 4% high amylolytic activity. The results were promising, demonstrating the production of the enzymes amylase and asparaginase by a large percentage of the isolated fungi, although at different levels, it can be considered a niche of microorganisms with great biotechnological potential to be explored. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-27T20:03:14Z 2020-03-04 2020-10-27T20:03:14Z 2020-02-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis info:eu-repo/semantics/dataset |
format |
bachelorThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SANTOS, Luís Felipe Oliva dos. Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase. 2019. 36 f. Trabalho de Conclusão de Curso (Graduação em Ciências Biológicas) – Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e Biológicas, Rondonópolis, 2019. http://bdm.ufmt.br/handle/1/1775 |
identifier_str_mv |
SANTOS, Luís Felipe Oliva dos. Triagem de fungos endofíticos de Moringa oleífera produtores de asparaginase e amilase. 2019. 36 f. Trabalho de Conclusão de Curso (Graduação em Ciências Biológicas) – Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e Biológicas, Rondonópolis, 2019. |
url |
http://bdm.ufmt.br/handle/1/1775 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Exatas e Naturais (ICEN) – Rondonópolis UFMT CUR - Rondonopólis Ciências Biológicas - CUR |
publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Exatas e Naturais (ICEN) – Rondonópolis UFMT CUR - Rondonopólis Ciências Biológicas - CUR |
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Universidade Federal de Mato Grosso (UFMT) |
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UFMT |
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Biblioteca Digital de Monografias da UFMT |
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Biblioteca Digital de Monografias da UFMT - Universidade Federal de Mato Grosso (UFMT) |
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