Caracterização fenotípica e genotípica de espécies de leveduras do complexo Cryptococcus isoladas de amostras da poeira de bibliotecas de Cuiabá e Várzea Grande e sua relação com árvores hospedeiras

• Autor(a) principal: Leite Júnior, Diniz Pereira
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Biblioteca Digital de Monografias da UFMT
• Texto Completo: http://bdm.ufmt.br/handle/1/386
• topic: CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA; Cryptococcus; Poeira; Bibliotecas; Ar; Habitat; Cryptococcus; Dust; Libraries; Air; Habitat

The Cryptococcus spp complex is composed of cosmopolitan distribution capped yeasts, including etiological agents of cryptococcosis. In the environment the fungus is found mainly in substrates of animal and vegetal origins. Human infection occurs through the inhalation of sexually propagated propagules present in the environment. In this work 42 samples were collected with the help of swab, gravitational exposure and Andersen sampler in dust found in the books belonging to the collection of seven libraries in the city of Cuiabá and Várzea Grande / Mato Grosso. The collected material was sown on Sabouraud agar, and after observing the characteristics compatible with colonies of creamy to mucoid aspect, they were isolated in Niger agar and cultured at temperatures of 27º and 37oC for 5-7 days. Identification of the isolated samples was performed by microscopic observation on fresh preparations stained by China ink, additional tests on CGB (L-Canavanine-Bromothymol blue-glycine), urea broth and carbohydrate assimilation tests (auxanogram) and Posterior molecular identification was performed in a specialized laboratory. Of the 42 samples collected from the dust of books, 1,306 (70%) of colonies were registered in libraries in Cuiabá and 559 (30%) in the libraries in Várzea Grande, of positive samples totaling 1,865 CFUs. The most frequently isolated species was C. gattii 46 (2.5%); Followed by C. neoformans 28 (1.5%, C. terreus 15 (0.8%), C. luteolus 12 (0.6%) and C. unigutullatus (0.5%), the remaining 94% are identified. The high biodiversity of yeasts of the genus Cryptococcus spp, isolated from several environmental sources in urban areas of Brazil, suggests the possibility of immunocompromised and even healthy individuals coming into contact with multiple sources of contagion daily throughout their lives This study points to the possible acquisition of the isolated dust fungus in library collections, due to the continuous exposure of the employees working in these environments and individuals visiting these places to the microniche present in the librarian environment.