Análise morfométrica de ratos Wistar infectados por Strongyloides venezuelensis

• Autor(a) principal: Figueiredo, Anna Júlia Fernandes
• Data de Publicação: 2020
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Biblioteca Digital de Monografias da UFMT
• Texto Completo: http://bdm.ufmt.br/handle/1/2003
• topic: CNPQ::OUTROS::BIOMEDICINA; estrongiloidíase; relação parasito-hospedeiro; morfologia; Strongyloides spp; strongyloidiasis; host-parasite relationship; morphology; Strongyloides spp

Strongyloidiasis is an intestinal parasitosis caused by the parasite Strongyloides spp. This parasitosis is mainly related to the heart, lung, small intestine, and stomach and secondarily to other organs such as the brain and kidneys. The importance of this work is to understand - through the intersection between morphology and parasitology - the host-parasite relationship. Its purpose was to perform morphometric analysis of Wistar rats infected with S. venezuelensis. 26 Wistar rats (Rattus norvegicus) were used, divided into 3 groups: control (C; n=10); infected (I; submitted to infection with 2,000 S. venezuelensis larvae; n=10) and treated (TI; submitted to infection with 2,000 S. venezuelensis larvae and treated with ivermectin; n=6). Initially, the animals were dewormed with 1.5 ml of ivermectin 1% (Mogimec™) in 700 ml of water. Later group I and TI were infected (2,000 larvae), nevertheless, group TI was treated with ivermectin (0.2mg/kg) - by the gavage method - during the 21 days post infection. The analyses were performed every 3 days, from day 0 to 21 dpi. Duplicate anesthetics Ketamine (Cetamin™) and Xylazine (Calmiun™) administered to the animals. Formula x=4/3 x π x (A x B x C) was used to calculate the organ volume, where A= radius of length, B= radius of width, and C= radius of thickness. π=3.14. The organs analyzed were: spleen, heart, brain, stomach, liver, small intestine (SI), pancreas, lung, right kidney, left kidney, and thymus. Statistical analyses were performed using the t-test for analysis of variance ANOVA twoway™, ANOVA oneway™, and PRISM™ for graph assembly; p<0.05. The results showed a significant increase in body weight between groups I and TI when compared to group C. In stool weight, there was an increase in group I and TI when compared to group C. Considerable increase in small intestine (SI) weight was observed in group I and a decrease in group TI; these changes are related to the literature description of patients with chronic hyperinfection, who presented a series of symptoms such as diarrhea, abdominal pain, vomiting, swelling in the abdominal region, inflammation of the gastrointestinal tract, among others. In the pancreas, there was a significant increase in group I when compared to group C, and in the right kidney there was an increase in group I when compared to group C. There was an increase in the volume of the heart in group I when compared to group C in the stomach there was an increase in group I and TI when compared to C; in the brain and also in the left kidney. Therefore, efforts to fight infection interfere with the physiology of organs not directly related to strongyloidiasis and that parasite-host interaction can promote tolerance to helminth infection, showing that, all body structures are compromised in the response to an infection. In conclusion, Strongyloides venezuelensis causes morphological changes in the host organism, even if the infection has been controlled after 21 days in Wistar rats.