Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Monografias da UFMT |
Texto Completo: | http://bdm.ufmt.br/handle/1/3238 |
Resumo: | Plants have a great ability to produce chemical substances that can display a variety of biological activities. Therefore, Cedrela odorata L. is a plant native to the Amazon region, and its bark is widely used for the treatment of many diseases, and has been experimentally demonstrated to have an anti-hyperglycemic, antioxidant and hypolipidemic effect and is safe at doses evaluated in animals. However, safety is of fundamental importance in drug development and knowing the toxic mechanism is also essential in this process. Therefore, this study aimed to evaluate the cytotoxic effect of the hydroethanolic extract of the inner bark of Cedrela Odorata L. (HECo). For this, Chinese Hamster Ovary (CHO-K1) cells were cultured in Ham F-12 medium, supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin 10,000U antibiotic, kept in a CO2 oven at 37°C. Cells were treated for 24h with HECo at concentrations of 1000, 800, 400, 200 and 50 μg/mL. The cytotoxic effects of the extract on cell viability were tested using the resazurin method. In order to evaluate the lactate dehydrogenase extravasation, it was quantified in the culture medium after the treatments. As a negative control, cells were treated with triton X-100 at concentrations of 0.1, 1, 10 and 100 µM. For the positive control (100%), the cells were exposed only to the culture medium. Data are expressed as mean ± mean standard error and analyzed by one-way ANOVA, followed by Tukey post-test. We observed that EHCo at the three highest concentrations was able to reduce cell viability to 19%, 31 and 62%, when compared to the positive control. Treatment with triton X-100 at a concentration of 100 and 10 µM reduced cell viability by 19% and 63%, respectively. The effective dose to reduce cell viability by 50% was 560.86 µg/mL for EHCo and 56.54 µM for triton X-100. There was no difference in LDH extravasation when cells were treated with triton X-100. However, when treated with EHCo, enzyme extravasation was inversely proportional to concentration. Therefore, it is suggest EHCo has a cytotoxic effect, probably because it has high cell permeability, promoting necrosis in cells. |
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Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICACedrela odorataCultivo de célulasCitotoxicidadeLactato desidrogenaseCedrela odorataCell cultivationCytotoxicityLactate dehydrogenasePlants have a great ability to produce chemical substances that can display a variety of biological activities. Therefore, Cedrela odorata L. is a plant native to the Amazon region, and its bark is widely used for the treatment of many diseases, and has been experimentally demonstrated to have an anti-hyperglycemic, antioxidant and hypolipidemic effect and is safe at doses evaluated in animals. However, safety is of fundamental importance in drug development and knowing the toxic mechanism is also essential in this process. Therefore, this study aimed to evaluate the cytotoxic effect of the hydroethanolic extract of the inner bark of Cedrela Odorata L. (HECo). For this, Chinese Hamster Ovary (CHO-K1) cells were cultured in Ham F-12 medium, supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin 10,000U antibiotic, kept in a CO2 oven at 37°C. Cells were treated for 24h with HECo at concentrations of 1000, 800, 400, 200 and 50 μg/mL. The cytotoxic effects of the extract on cell viability were tested using the resazurin method. In order to evaluate the lactate dehydrogenase extravasation, it was quantified in the culture medium after the treatments. As a negative control, cells were treated with triton X-100 at concentrations of 0.1, 1, 10 and 100 µM. For the positive control (100%), the cells were exposed only to the culture medium. Data are expressed as mean ± mean standard error and analyzed by one-way ANOVA, followed by Tukey post-test. We observed that EHCo at the three highest concentrations was able to reduce cell viability to 19%, 31 and 62%, when compared to the positive control. Treatment with triton X-100 at a concentration of 100 and 10 µM reduced cell viability by 19% and 63%, respectively. The effective dose to reduce cell viability by 50% was 560.86 µg/mL for EHCo and 56.54 µM for triton X-100. There was no difference in LDH extravasation when cells were treated with triton X-100. However, when treated with EHCo, enzyme extravasation was inversely proportional to concentration. Therefore, it is suggest EHCo has a cytotoxic effect, probably because it has high cell permeability, promoting necrosis in cells.As plantas têm uma grande habilidade de produzir substâncias químicas que podem apresentar uma diversidade de atividades biológicas. Logo, a Cedrela odorata L. é uma planta nativa da região da Amazônia, e sua entrecasca é muito usada para tratamento de muitas doenças, e demonstrou experimentalmente possuir efeito anti-hiperglicêmico, antioxidante e hipolipidêmico e seguro nas doses avaliadas em animais. Entretanto, a segurança é de fundamental importância no desenvolvimento de fármacos e conhecer o mecanismo tóxico também é primordial neste processo. Sendo assim, este trabalho teve como objetivo avaliar o efeito citotóxico do extrato hidroetanólico da entrecasca da Cedrela Odorata L (HECo). Para isso, células da linhagem de ovário de Hamster Chinês (CHO-K1) foram cultivadas em meio Ham F-12, suplementadas com 10% de soro fetal bovino e 1% de antibiótico penicilinaestreptomicina 10.000U, mantidas em estufa de CO2 à 37°C. As células foram tratadas por 24h com o HECo nas concentrações de 1000, 800, 400, 200 e 50 μg/mL. Os efeitos citotoxicos do extrato sobre a viabilidade celular foram testados usando o método de resazurina. Afim de se avaliar o extravasamento da lactato desidrogenase, a mesma foi quantificada no meio de cultura após os tratamentos. Como controle negativo, as células foram tratadas com triton X-100 nas concentrações de 0,1, 1, 10 e 100 µM. Para o controle positivo (100%), as células foram expostas somente ao meio de cultura. Os dados estão expressos como média ± erro médio padrão e analisados por ANOVA uma via, seguido do Tukey post-test. Observamos que o EHCo nas três maiores concentrações foi capaz de reduzir a viabilidade celular para 19%, 31 e 62%, quando comparado com o controle positivo. O tratamento com o triton X-100 na concentração de 100 e 10 µM promoveu a redução da viabilidade celular em 19% e 63%, respectivamente. A dose efetiva para reduzir a viabilidade celular em 50% foi de 560,86 µg/mL para o EHCo e de 56,54 µM para o triton X-100. Não houve diferença no extravasamento de LDH quando as células foram tratadas com triton X-100. No entanto, quando tratadas com o EHCo, o extravasamento da enzima foi inversamente proporcional a concentração. Portanto, sugere- se que o EHCo possui efeito citotóxico, provavelmente por possuir alta permeabilidade celular, promovendo necrose nas células.Universidade Federal de Mato GrossoBrasilInstituto de Ciências Exatas e da Terra (ICET)UFMT CUC - CuiabáQuímica - CUC - BachareladoPereira, Mayara Peron019.512.681-56http://lattes.cnpq.br/7133055047958148Almeida, Fhelipe Jolner Souza de045.249.221-13http://lattes.cnpq.br/6915192907405882Pereira, Mayara Peron019.512.681-56http://lattes.cnpq.br/7133055047958148Siqueira, Juliany Torres022.703.831-25http://lattes.cnpq.br/3130847230380790Ribeiro, Tereza Auxiliadora Nascimento030.639.426-00http://lattes.cnpq.br/1866455078888688Silva, Viviani de Brito Massad Gomes da2023-07-07T18:51:11Z2022-04-142023-07-07T18:51:11Z2022-03-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/datasetSILVA, Viviani de Brito Massad Gomes da. Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. 2022. 46 f. Trabalho de Conclusão de Curso (Graduação em Química Bacharelado) - Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e da Terra, Cuiabá, 2022.http://bdm.ufmt.br/handle/1/3238porinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Monografias da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2023-07-11T07:06:22Zoai:localhost:1/3238Biblioteca Digital de Monografiahttps://bdm.ufmt.br/PUBhttp://200.129.241.122/oai/requestopendoar:2023-07-11T07:06:22falseBiblioteca Digital de Monografiahttps://bdm.ufmt.br/PUBhttp://200.129.241.122/oai/requestbibliotecacentral@ufmt.br||opendoar:2023-07-11T07:06:22Biblioteca Digital de Monografias da UFMT - Universidade Federal de Mato Grosso (UFMT)false |
dc.title.none.fl_str_mv |
Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. |
title |
Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. |
spellingShingle |
Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. Silva, Viviani de Brito Massad Gomes da CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA Cedrela odorata Cultivo de células Citotoxicidade Lactato desidrogenase Cedrela odorata Cell cultivation Cytotoxicity Lactate dehydrogenase |
title_short |
Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. |
title_full |
Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. |
title_fullStr |
Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. |
title_full_unstemmed |
Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. |
title_sort |
Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. |
author |
Silva, Viviani de Brito Massad Gomes da |
author_facet |
Silva, Viviani de Brito Massad Gomes da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Pereira, Mayara Peron 019.512.681-56 http://lattes.cnpq.br/7133055047958148 Almeida, Fhelipe Jolner Souza de 045.249.221-13 http://lattes.cnpq.br/6915192907405882 Pereira, Mayara Peron 019.512.681-56 http://lattes.cnpq.br/7133055047958148 Siqueira, Juliany Torres 022.703.831-25 http://lattes.cnpq.br/3130847230380790 Ribeiro, Tereza Auxiliadora Nascimento 030.639.426-00 http://lattes.cnpq.br/1866455078888688 |
dc.contributor.author.fl_str_mv |
Silva, Viviani de Brito Massad Gomes da |
dc.subject.por.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA Cedrela odorata Cultivo de células Citotoxicidade Lactato desidrogenase Cedrela odorata Cell cultivation Cytotoxicity Lactate dehydrogenase |
topic |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA Cedrela odorata Cultivo de células Citotoxicidade Lactato desidrogenase Cedrela odorata Cell cultivation Cytotoxicity Lactate dehydrogenase |
description |
Plants have a great ability to produce chemical substances that can display a variety of biological activities. Therefore, Cedrela odorata L. is a plant native to the Amazon region, and its bark is widely used for the treatment of many diseases, and has been experimentally demonstrated to have an anti-hyperglycemic, antioxidant and hypolipidemic effect and is safe at doses evaluated in animals. However, safety is of fundamental importance in drug development and knowing the toxic mechanism is also essential in this process. Therefore, this study aimed to evaluate the cytotoxic effect of the hydroethanolic extract of the inner bark of Cedrela Odorata L. (HECo). For this, Chinese Hamster Ovary (CHO-K1) cells were cultured in Ham F-12 medium, supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin 10,000U antibiotic, kept in a CO2 oven at 37°C. Cells were treated for 24h with HECo at concentrations of 1000, 800, 400, 200 and 50 μg/mL. The cytotoxic effects of the extract on cell viability were tested using the resazurin method. In order to evaluate the lactate dehydrogenase extravasation, it was quantified in the culture medium after the treatments. As a negative control, cells were treated with triton X-100 at concentrations of 0.1, 1, 10 and 100 µM. For the positive control (100%), the cells were exposed only to the culture medium. Data are expressed as mean ± mean standard error and analyzed by one-way ANOVA, followed by Tukey post-test. We observed that EHCo at the three highest concentrations was able to reduce cell viability to 19%, 31 and 62%, when compared to the positive control. Treatment with triton X-100 at a concentration of 100 and 10 µM reduced cell viability by 19% and 63%, respectively. The effective dose to reduce cell viability by 50% was 560.86 µg/mL for EHCo and 56.54 µM for triton X-100. There was no difference in LDH extravasation when cells were treated with triton X-100. However, when treated with EHCo, enzyme extravasation was inversely proportional to concentration. Therefore, it is suggest EHCo has a cytotoxic effect, probably because it has high cell permeability, promoting necrosis in cells. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-14 2022-03-18 2023-07-07T18:51:11Z 2023-07-07T18:51:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis info:eu-repo/semantics/dataset |
format |
bachelorThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA, Viviani de Brito Massad Gomes da. Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. 2022. 46 f. Trabalho de Conclusão de Curso (Graduação em Química Bacharelado) - Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e da Terra, Cuiabá, 2022. http://bdm.ufmt.br/handle/1/3238 |
identifier_str_mv |
SILVA, Viviani de Brito Massad Gomes da. Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. 2022. 46 f. Trabalho de Conclusão de Curso (Graduação em Química Bacharelado) - Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e da Terra, Cuiabá, 2022. |
url |
http://bdm.ufmt.br/handle/1/3238 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Mato Grosso Brasil Instituto de Ciências Exatas e da Terra (ICET) UFMT CUC - Cuiabá Química - CUC - Bacharelado |
publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Exatas e da Terra (ICET) UFMT CUC - Cuiabá Química - CUC - Bacharelado |
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reponame:Biblioteca Digital de Monografias da UFMT instname:Universidade Federal de Mato Grosso (UFMT) instacron:UFMT |
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Universidade Federal de Mato Grosso (UFMT) |
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Biblioteca Digital de Monografias da UFMT - Universidade Federal de Mato Grosso (UFMT) |
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