Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.

Detalhes bibliográficos
Autor(a) principal: Silva, Viviani de Brito Massad Gomes da
Data de Publicação: 2022
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Biblioteca Digital de Monografias da UFMT
Texto Completo: http://bdm.ufmt.br/handle/1/3238
Resumo: Plants have a great ability to produce chemical substances that can display a variety of biological activities. Therefore, Cedrela odorata L. is a plant native to the Amazon region, and its bark is widely used for the treatment of many diseases, and has been experimentally demonstrated to have an anti-hyperglycemic, antioxidant and hypolipidemic effect and is safe at doses evaluated in animals. However, safety is of fundamental importance in drug development and knowing the toxic mechanism is also essential in this process. Therefore, this study aimed to evaluate the cytotoxic effect of the hydroethanolic extract of the inner bark of Cedrela Odorata L. (HECo). For this, Chinese Hamster Ovary (CHO-K1) cells were cultured in Ham F-12 medium, supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin 10,000U antibiotic, kept in a CO2 oven at 37°C. Cells were treated for 24h with HECo at concentrations of 1000, 800, 400, 200 and 50 μg/mL. The cytotoxic effects of the extract on cell viability were tested using the resazurin method. In order to evaluate the lactate dehydrogenase extravasation, it was quantified in the culture medium after the treatments. As a negative control, cells were treated with triton X-100 at concentrations of 0.1, 1, 10 and 100 µM. For the positive control (100%), the cells were exposed only to the culture medium. Data are expressed as mean ± mean standard error and analyzed by one-way ANOVA, followed by Tukey post-test. We observed that EHCo at the three highest concentrations was able to reduce cell viability to 19%, 31 and 62%, when compared to the positive control. Treatment with triton X-100 at a concentration of 100 and 10 µM reduced cell viability by 19% and 63%, respectively. The effective dose to reduce cell viability by 50% was 560.86 µg/mL for EHCo and 56.54 µM for triton X-100. There was no difference in LDH extravasation when cells were treated with triton X-100. However, when treated with EHCo, enzyme extravasation was inversely proportional to concentration. Therefore, it is suggest EHCo has a cytotoxic effect, probably because it has high cell permeability, promoting necrosis in cells.
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spelling Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICACedrela odorataCultivo de célulasCitotoxicidadeLactato desidrogenaseCedrela odorataCell cultivationCytotoxicityLactate dehydrogenasePlants have a great ability to produce chemical substances that can display a variety of biological activities. Therefore, Cedrela odorata L. is a plant native to the Amazon region, and its bark is widely used for the treatment of many diseases, and has been experimentally demonstrated to have an anti-hyperglycemic, antioxidant and hypolipidemic effect and is safe at doses evaluated in animals. However, safety is of fundamental importance in drug development and knowing the toxic mechanism is also essential in this process. Therefore, this study aimed to evaluate the cytotoxic effect of the hydroethanolic extract of the inner bark of Cedrela Odorata L. (HECo). For this, Chinese Hamster Ovary (CHO-K1) cells were cultured in Ham F-12 medium, supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin 10,000U antibiotic, kept in a CO2 oven at 37°C. Cells were treated for 24h with HECo at concentrations of 1000, 800, 400, 200 and 50 μg/mL. The cytotoxic effects of the extract on cell viability were tested using the resazurin method. In order to evaluate the lactate dehydrogenase extravasation, it was quantified in the culture medium after the treatments. As a negative control, cells were treated with triton X-100 at concentrations of 0.1, 1, 10 and 100 µM. For the positive control (100%), the cells were exposed only to the culture medium. Data are expressed as mean ± mean standard error and analyzed by one-way ANOVA, followed by Tukey post-test. We observed that EHCo at the three highest concentrations was able to reduce cell viability to 19%, 31 and 62%, when compared to the positive control. Treatment with triton X-100 at a concentration of 100 and 10 µM reduced cell viability by 19% and 63%, respectively. The effective dose to reduce cell viability by 50% was 560.86 µg/mL for EHCo and 56.54 µM for triton X-100. There was no difference in LDH extravasation when cells were treated with triton X-100. However, when treated with EHCo, enzyme extravasation was inversely proportional to concentration. Therefore, it is suggest EHCo has a cytotoxic effect, probably because it has high cell permeability, promoting necrosis in cells.As plantas têm uma grande habilidade de produzir substâncias químicas que podem apresentar uma diversidade de atividades biológicas. Logo, a Cedrela odorata L. é uma planta nativa da região da Amazônia, e sua entrecasca é muito usada para tratamento de muitas doenças, e demonstrou experimentalmente possuir efeito anti-hiperglicêmico, antioxidante e hipolipidêmico e seguro nas doses avaliadas em animais. Entretanto, a segurança é de fundamental importância no desenvolvimento de fármacos e conhecer o mecanismo tóxico também é primordial neste processo. Sendo assim, este trabalho teve como objetivo avaliar o efeito citotóxico do extrato hidroetanólico da entrecasca da Cedrela Odorata L (HECo). Para isso, células da linhagem de ovário de Hamster Chinês (CHO-K1) foram cultivadas em meio Ham F-12, suplementadas com 10% de soro fetal bovino e 1% de antibiótico penicilinaestreptomicina 10.000U, mantidas em estufa de CO2 à 37°C. As células foram tratadas por 24h com o HECo nas concentrações de 1000, 800, 400, 200 e 50 μg/mL. Os efeitos citotoxicos do extrato sobre a viabilidade celular foram testados usando o método de resazurina. Afim de se avaliar o extravasamento da lactato desidrogenase, a mesma foi quantificada no meio de cultura após os tratamentos. Como controle negativo, as células foram tratadas com triton X-100 nas concentrações de 0,1, 1, 10 e 100 µM. Para o controle positivo (100%), as células foram expostas somente ao meio de cultura. Os dados estão expressos como média ± erro médio padrão e analisados por ANOVA uma via, seguido do Tukey post-test. Observamos que o EHCo nas três maiores concentrações foi capaz de reduzir a viabilidade celular para 19%, 31 e 62%, quando comparado com o controle positivo. O tratamento com o triton X-100 na concentração de 100 e 10 µM promoveu a redução da viabilidade celular em 19% e 63%, respectivamente. A dose efetiva para reduzir a viabilidade celular em 50% foi de 560,86 µg/mL para o EHCo e de 56,54 µM para o triton X-100. Não houve diferença no extravasamento de LDH quando as células foram tratadas com triton X-100. No entanto, quando tratadas com o EHCo, o extravasamento da enzima foi inversamente proporcional a concentração. Portanto, sugere- se que o EHCo possui efeito citotóxico, provavelmente por possuir alta permeabilidade celular, promovendo necrose nas células.Universidade Federal de Mato GrossoBrasilInstituto de Ciências Exatas e da Terra (ICET)UFMT CUC - CuiabáQuímica - CUC - BachareladoPereira, Mayara Peron019.512.681-56http://lattes.cnpq.br/7133055047958148Almeida, Fhelipe Jolner Souza de045.249.221-13http://lattes.cnpq.br/6915192907405882Pereira, Mayara Peron019.512.681-56http://lattes.cnpq.br/7133055047958148Siqueira, Juliany Torres022.703.831-25http://lattes.cnpq.br/3130847230380790Ribeiro, Tereza Auxiliadora Nascimento030.639.426-00http://lattes.cnpq.br/1866455078888688Silva, Viviani de Brito Massad Gomes da2023-07-07T18:51:11Z2022-04-142023-07-07T18:51:11Z2022-03-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/datasetSILVA, Viviani de Brito Massad Gomes da. Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. 2022. 46 f. Trabalho de Conclusão de Curso (Graduação em Química Bacharelado) - Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e da Terra, Cuiabá, 2022.http://bdm.ufmt.br/handle/1/3238porinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Monografias da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2023-07-11T07:06:22Zoai:localhost:1/3238Biblioteca Digital de Monografiahttps://bdm.ufmt.br/PUBhttp://200.129.241.122/oai/requestopendoar:2023-07-11T07:06:22falseBiblioteca Digital de Monografiahttps://bdm.ufmt.br/PUBhttp://200.129.241.122/oai/requestbibliotecacentral@ufmt.br||opendoar:2023-07-11T07:06:22Biblioteca Digital de Monografias da UFMT - Universidade Federal de Mato Grosso (UFMT)false
dc.title.none.fl_str_mv Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.
title Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.
spellingShingle Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.
Silva, Viviani de Brito Massad Gomes da
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
Cedrela odorata
Cultivo de células
Citotoxicidade
Lactato desidrogenase
Cedrela odorata
Cell cultivation
Cytotoxicity
Lactate dehydrogenase
title_short Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.
title_full Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.
title_fullStr Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.
title_full_unstemmed Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.
title_sort Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L.
author Silva, Viviani de Brito Massad Gomes da
author_facet Silva, Viviani de Brito Massad Gomes da
author_role author
dc.contributor.none.fl_str_mv Pereira, Mayara Peron
019.512.681-56
http://lattes.cnpq.br/7133055047958148
Almeida, Fhelipe Jolner Souza de
045.249.221-13
http://lattes.cnpq.br/6915192907405882
Pereira, Mayara Peron
019.512.681-56
http://lattes.cnpq.br/7133055047958148
Siqueira, Juliany Torres
022.703.831-25
http://lattes.cnpq.br/3130847230380790
Ribeiro, Tereza Auxiliadora Nascimento
030.639.426-00
http://lattes.cnpq.br/1866455078888688
dc.contributor.author.fl_str_mv Silva, Viviani de Brito Massad Gomes da
dc.subject.por.fl_str_mv CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
Cedrela odorata
Cultivo de células
Citotoxicidade
Lactato desidrogenase
Cedrela odorata
Cell cultivation
Cytotoxicity
Lactate dehydrogenase
topic CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
Cedrela odorata
Cultivo de células
Citotoxicidade
Lactato desidrogenase
Cedrela odorata
Cell cultivation
Cytotoxicity
Lactate dehydrogenase
description Plants have a great ability to produce chemical substances that can display a variety of biological activities. Therefore, Cedrela odorata L. is a plant native to the Amazon region, and its bark is widely used for the treatment of many diseases, and has been experimentally demonstrated to have an anti-hyperglycemic, antioxidant and hypolipidemic effect and is safe at doses evaluated in animals. However, safety is of fundamental importance in drug development and knowing the toxic mechanism is also essential in this process. Therefore, this study aimed to evaluate the cytotoxic effect of the hydroethanolic extract of the inner bark of Cedrela Odorata L. (HECo). For this, Chinese Hamster Ovary (CHO-K1) cells were cultured in Ham F-12 medium, supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin 10,000U antibiotic, kept in a CO2 oven at 37°C. Cells were treated for 24h with HECo at concentrations of 1000, 800, 400, 200 and 50 μg/mL. The cytotoxic effects of the extract on cell viability were tested using the resazurin method. In order to evaluate the lactate dehydrogenase extravasation, it was quantified in the culture medium after the treatments. As a negative control, cells were treated with triton X-100 at concentrations of 0.1, 1, 10 and 100 µM. For the positive control (100%), the cells were exposed only to the culture medium. Data are expressed as mean ± mean standard error and analyzed by one-way ANOVA, followed by Tukey post-test. We observed that EHCo at the three highest concentrations was able to reduce cell viability to 19%, 31 and 62%, when compared to the positive control. Treatment with triton X-100 at a concentration of 100 and 10 µM reduced cell viability by 19% and 63%, respectively. The effective dose to reduce cell viability by 50% was 560.86 µg/mL for EHCo and 56.54 µM for triton X-100. There was no difference in LDH extravasation when cells were treated with triton X-100. However, when treated with EHCo, enzyme extravasation was inversely proportional to concentration. Therefore, it is suggest EHCo has a cytotoxic effect, probably because it has high cell permeability, promoting necrosis in cells.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-14
2022-03-18
2023-07-07T18:51:11Z
2023-07-07T18:51:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/dataset
format bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, Viviani de Brito Massad Gomes da. Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. 2022. 46 f. Trabalho de Conclusão de Curso (Graduação em Química Bacharelado) - Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e da Terra, Cuiabá, 2022.
http://bdm.ufmt.br/handle/1/3238
identifier_str_mv SILVA, Viviani de Brito Massad Gomes da. Potencial citotóxico do extrato bruto hidroetanólico da entrecasca da Cedrela odorata L. 2022. 46 f. Trabalho de Conclusão de Curso (Graduação em Química Bacharelado) - Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e da Terra, Cuiabá, 2022.
url http://bdm.ufmt.br/handle/1/3238
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Instituto de Ciências Exatas e da Terra (ICET)
UFMT CUC - Cuiabá
Química - CUC - Bacharelado
publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Instituto de Ciências Exatas e da Terra (ICET)
UFMT CUC - Cuiabá
Química - CUC - Bacharelado
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Monografias da UFMT
instname:Universidade Federal de Mato Grosso (UFMT)
instacron:UFMT
instname_str Universidade Federal de Mato Grosso (UFMT)
instacron_str UFMT
institution UFMT
reponame_str Biblioteca Digital de Monografias da UFMT
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repository.name.fl_str_mv Biblioteca Digital de Monografias da UFMT - Universidade Federal de Mato Grosso (UFMT)
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