AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARI

Detalhes bibliográficos
Autor(a) principal: Cuchi, Giovana
Data de Publicação: 2023
Outros Autores: Wolf, Mariéllen Schmith, Pedri, Eliane Cristina Moreno de, Zortéa, Kelli Évelin Müller, Alves, Anderson Ortiz, Costa de Arruda , Joari, Silva, Carolina Joana da, Rossi, Ana Aparecida Bandini
Tipo de documento: Artigo
Idioma: por
Título da fonte: Nativa (Sinop)
Texto Completo: https://periodicoscientificos.ufmt.br/ojs/index.php/nativa/article/view/15205
Resumo: The species Garcinia brasiliensis has various scientifically proven applications in folk medicine, however, the lack of dissemination of these characteristics of the species is a current problem. Among the studies that can enhance this knowledge is the genetic diversity study, for which the use of ISSR molecular markers is recommended for being reliable, rapid, and economical. Therefore, this study aimed to select and classify dominant molecular markers for studies with the G. brasiliensis species. For this, total DNA was extracted from 40 bacupari individuals. Two individuals were selected to perform ISSR primer amplification tests via PCR. 31 primers were tested, which were classified regarding their amplification quality as good, poor, or absent. Out of the 31 tested primers, 13 showed good results regarding the presence and quality of the bands, therefore, they were indicated for use in research aiming the molecular characterization and evaluation of genetic diversity of the species Garcinia brasiliensis.
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spelling AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARIDiversidade GenéticaGarcinia brasiliensisPantanalThe species Garcinia brasiliensis has various scientifically proven applications in folk medicine, however, the lack of dissemination of these characteristics of the species is a current problem. Among the studies that can enhance this knowledge is the genetic diversity study, for which the use of ISSR molecular markers is recommended for being reliable, rapid, and economical. Therefore, this study aimed to select and classify dominant molecular markers for studies with the G. brasiliensis species. For this, total DNA was extracted from 40 bacupari individuals. Two individuals were selected to perform ISSR primer amplification tests via PCR. 31 primers were tested, which were classified regarding their amplification quality as good, poor, or absent. Out of the 31 tested primers, 13 showed good results regarding the presence and quality of the bands, therefore, they were indicated for use in research aiming the molecular characterization and evaluation of genetic diversity of the species Garcinia brasiliensis.A espécie Garcinia brasiliensis apresenta diversas aplicações na medicina popular comprovadas cientificamente, entretanto, a falta de divulgação sobre essas características da espécie é um problema atual. Dentre os estudos que podem alavancar esse conhecimento está o da diversidade genética, para isso, o uso de marcadores moleculares ISSR é indicado por serem confiáveis, rápidos e econômicos. Sendo assim, este trabalho teve como objetivo selecionar e classificar marcadores moleculares dominantes para estudos com a espécie G. brasiliensis. Para isso, foi extraído o DNA total de 40 indivíduos de bacupari, dos quais foram selecionados dois, que apresentassem bandas bem definidas e sem arraste, para realizar testes de amplificação de primers ISSR via PCR. Ao todo, foram testados 31 primers, os quais foram classificados quanto sua qualidade de amplificação, utilizando os padrões bom, ruim e ausente. Dos 31 primers testados, 13 apresentaram bons resultados quanto à presença e qualidade das bandas, portanto, indicados para o uso em pesquisas que visem à caracterização molecular e avaliação da diversidade genética da espécie Garcinia brasiliensis. Palavras-chave: Garcinia brasiliensis; marcador molecular; Pantanal.   Evaluation of ISSR markers for molecular studies of bacupari   ABSTRACT: The species Garcinia brasiliensis has various scientifically proven applications in folk medicine, however, the lack of dissemination of these species characteristics is a current problem. Among the studies that can enhance this knowledge is genetic diversity, for which ISSR molecular markers application is recommended as it is reliable, rapid, and economical. Therefore, this study aimed to select and classify dominant molecular markers for G. brasiliensis research. For this, total DNA was extracted from 40 bacupari individuals. Two individuals were selected to perform ISSR primer amplification tests by PCR. 31 primers were tested, which classified their amplification quality as good, poor, or absent. Out of the 31 tested primers, 13 showed good results regarding the presence and band quality, therefore, they were indicated for research use, aiming molecular characterization and Garcinia brasiliensis genetic diversity evaluation. Keywords: Garcinia brasiliensis; molecular marker; Pantanal.Universidade Federal de Mato Grosso2023-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicoscientificos.ufmt.br/ojs/index.php/nativa/article/view/1520510.31413/nat.v11i3.15205Nativa; v. 11 n. 3 (2023); 368-373Nativa; Vol. 11 Núm. 3 (2023); 368-373Nativa; Vol. 11 No. 3 (2023); 368-3732318-7670reponame:Nativa (Sinop)instname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMTporhttps://periodicoscientificos.ufmt.br/ojs/index.php/nativa/article/view/15205/12636Copyright (c) 2023 Nativahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCuchi, GiovanaWolf, Mariéllen SchmithPedri, Eliane Cristina Moreno deZortéa, Kelli Évelin Müller Alves, Anderson OrtizCosta de Arruda , Joari Silva, Carolina Joana daRossi, Ana Aparecida Bandini 2023-09-15T13:53:37Zoai:periodicoscientificos.ufmt.br:article/15205Revistahttps://periodicoscientificos.ufmt.br/ojs/index.php/nativaPUBhttps://periodicoscientificos.ufmt.br/ojs/index.php/nativa/oai||rrmelo2@yahoo.com.br2318-76702318-7670opendoar:2023-09-15T13:53:37Nativa (Sinop) - Universidade Federal de Mato Grosso (UFMT)false
dc.title.none.fl_str_mv AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARI
title AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARI
spellingShingle AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARI
Cuchi, Giovana
Diversidade Genética
Garcinia brasiliensis
Pantanal
title_short AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARI
title_full AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARI
title_fullStr AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARI
title_full_unstemmed AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARI
title_sort AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARI
author Cuchi, Giovana
author_facet Cuchi, Giovana
Wolf, Mariéllen Schmith
Pedri, Eliane Cristina Moreno de
Zortéa, Kelli Évelin Müller
Alves, Anderson Ortiz
Costa de Arruda , Joari
Silva, Carolina Joana da
Rossi, Ana Aparecida Bandini
author_role author
author2 Wolf, Mariéllen Schmith
Pedri, Eliane Cristina Moreno de
Zortéa, Kelli Évelin Müller
Alves, Anderson Ortiz
Costa de Arruda , Joari
Silva, Carolina Joana da
Rossi, Ana Aparecida Bandini
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cuchi, Giovana
Wolf, Mariéllen Schmith
Pedri, Eliane Cristina Moreno de
Zortéa, Kelli Évelin Müller
Alves, Anderson Ortiz
Costa de Arruda , Joari
Silva, Carolina Joana da
Rossi, Ana Aparecida Bandini
dc.subject.por.fl_str_mv Diversidade Genética
Garcinia brasiliensis
Pantanal
topic Diversidade Genética
Garcinia brasiliensis
Pantanal
description The species Garcinia brasiliensis has various scientifically proven applications in folk medicine, however, the lack of dissemination of these characteristics of the species is a current problem. Among the studies that can enhance this knowledge is the genetic diversity study, for which the use of ISSR molecular markers is recommended for being reliable, rapid, and economical. Therefore, this study aimed to select and classify dominant molecular markers for studies with the G. brasiliensis species. For this, total DNA was extracted from 40 bacupari individuals. Two individuals were selected to perform ISSR primer amplification tests via PCR. 31 primers were tested, which were classified regarding their amplification quality as good, poor, or absent. Out of the 31 tested primers, 13 showed good results regarding the presence and quality of the bands, therefore, they were indicated for use in research aiming the molecular characterization and evaluation of genetic diversity of the species Garcinia brasiliensis.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicoscientificos.ufmt.br/ojs/index.php/nativa/article/view/15205
10.31413/nat.v11i3.15205
url https://periodicoscientificos.ufmt.br/ojs/index.php/nativa/article/view/15205
identifier_str_mv 10.31413/nat.v11i3.15205
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicoscientificos.ufmt.br/ojs/index.php/nativa/article/view/15205/12636
dc.rights.driver.fl_str_mv Copyright (c) 2023 Nativa
https://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Nativa
https://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Mato Grosso
publisher.none.fl_str_mv Universidade Federal de Mato Grosso
dc.source.none.fl_str_mv Nativa; v. 11 n. 3 (2023); 368-373
Nativa; Vol. 11 Núm. 3 (2023); 368-373
Nativa; Vol. 11 No. 3 (2023); 368-373
2318-7670
reponame:Nativa (Sinop)
instname:Universidade Federal de Mato Grosso (UFMT)
instacron:UFMT
instname_str Universidade Federal de Mato Grosso (UFMT)
instacron_str UFMT
institution UFMT
reponame_str Nativa (Sinop)
collection Nativa (Sinop)
repository.name.fl_str_mv Nativa (Sinop) - Universidade Federal de Mato Grosso (UFMT)
repository.mail.fl_str_mv ||rrmelo2@yahoo.com.br
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